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Description: Potent Smo antagonist
Chemical Name: N,N'-1,4-Phenylenebis[2,5-dichlorobenzamide]
Purity: ≥99% (HPLC)
Literature (1)

Biological Activity for IHR 1

IHR 1 is a potent Smo antagonist (IC50 = 7.6 nM). Selectively inhibits Hedgehog signaling over Wnt and Notch signaling pathways. Blocks Smo accumulation in primary cilium in vitro.

Fluorescent Analog also available.

Technical Data for IHR 1

M. Wt 454.13
Formula C20H12Cl4N2O2
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 548779-60-8
PubChem ID 2167042
Smiles ClC1=CC(C(NC2=CC=C(NC(C3=C(Cl)C=CC(Cl)=C3)=O)C=C2)=O)=C(Cl)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for IHR 1

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 9.08 20 with gentle warming

Preparing Stock Solutions for IHR 1

The following data is based on the product molecular weight 454.13. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 11.01 mL 55.05 mL 110.1 mL
1 mM 2.2 mL 11.01 mL 22.02 mL
2 mM 1.1 mL 5.51 mL 11.01 mL
10 mM 0.22 mL 1.1 mL 2.2 mL

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References for IHR 1

References are publications that support the biological activity of the product.

Fan et al (2014) The Hedgehog pathway effector smoothened exhibits signaling competency in the absence of ciliary accumulation. Chem.Biol. 21 1680 PMID: 25484239

If you know of a relevant reference for IHR 1, please let us know.

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Citations for IHR 1

Citations are publications that use Tocris products.

Currently there are no citations for IHR 1. Do you know of a great paper that uses IHR 1 from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

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