HPI 1

Pricing Availability Delivery Time Qty
Cat.No. 3839 - HPI 1 | C27H29NO6 | CAS No. 599150-20-6
Description: Inhibits Hedgehog signaling
Chemical Name: 1,4,5,6,7,8-Hexahydro-4-(3-hydroxyphenyl)-7-(2-methoxyphenyl)-2-methyl-5-oxo-3-quinolinecarboxylic acid 2-methoxyethyl ester
Purity: ≥95% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Hedgehog (Hh) signaling inhibitor. Inhibits Sonic hedgehog (Shh)-, SAG- and Gli-induced Hh pathway activation in Shh-LIGHT2 cells (IC50 values are 1.5, 1.5, 4 and 6 μM for Shh-, SAG-, Gli2- and Gli1-induced activation). Also inhibits Hh pathway activation in SmoM2-LIGHT cells (IC50 = 2.5 μM); inhibits the proliferation of cerebellar granule neuron precursors expressing SmoM2. Does not inhibit Wnt signaling.

Technical Data

M. Wt 463.52
Formula C27H29NO6
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 599150-20-6
PubChem ID 2926768
InChI Key ZXSWZQSYZYMZKS-UHFFFAOYSA-N
Smiles CC2=C(C(OCCOC)=O)C(C3=CC(O)=CC=C3)C1=C(N2)CC(C4=C(OC)C=CC=C4)CC1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 46.35 100
ethanol 46.35 100

Preparing Stock Solutions

The following data is based on the product molecular weight 463.52. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.16 mL 10.79 mL 21.57 mL
5 mM 0.43 mL 2.16 mL 4.31 mL
10 mM 0.22 mL 1.08 mL 2.16 mL
50 mM 0.04 mL 0.22 mL 0.43 mL

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Product Datasheets

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References

References are publications that support the products' biological activity.

Hyman et al (2009) Small-molecule inhibitors reveal multiple strategies for Hedgehog pathway blockade. Proc.Natl.Acad.Sci.USA 106 14132 PMID: 19666565


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Literature in this Area

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Stem Cells

Stem Cells Scientific Review

Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.

Pathways for HPI 1

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