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Submit ReviewHM Janelia Fluor® 526, SE
Description: Fluorogenic spontaneously blinking yellow-emitting dye; for SMLSM; λabsλem 526/550 nm
Alternative Names: HM-JF526 NHS
Chemical Name: 2,5-Dioxopyrrolidin-1-yl 3',6'-bis(3,3-difluoroazetidin-1-yl)-2',7'-difluoro-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylate
Purity: ≥90% (HPLC)
Biological Activity for HM Janelia Fluor® 526, SE
HM Janelia Fluor® 526, SE is a fluorogenic spontaneously blinking green-emitting dye; hydroxymethyl derivative of JF526. Suitable for super-resolution imaging including dSTORM, STED and single-molecule localization spectroscopy (SMLSM). The spontaneous blinking properties of HM Janelia Fluor® 526, avoids the requirement for strongly reducing dSTORM buffers. Also suitable for conventional confocal microscopy and live cell imaging. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®.
To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for HM Janelia Fluor® 526, SE
| Emission Color | Yellow |
|---|---|
| λabs | 526 nm |
| λem | 550 nm |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Application | dSTORM, SMLSM, STED, SIM, confocal microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for HM Janelia Fluor® 526, SE
| M. Wt | 645.51 |
| Formula | C31H21F6N3O6 |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 2376841-30-2 |
| InChI Key | HJUSNAUMCROPEK-UHFFFAOYSA-N |
| Smiles | FC1=C(N2CC(F)(C2)F)C=C3OC4=CC(N5CC(F)(C5)F)=C(C=C4C6(C3=C1)OCC7=CC=C(C(ON8C(CCC8=O)=O)=O)C=C67)F |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for HM Janelia Fluor® 526, SE
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 12.91 | 20 |
Preparing Stock Solutions for HM Janelia Fluor® 526, SE
The following data is based on the product molecular weight 645.51. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 7.75 mL | 38.73 mL | 77.46 mL |
| 1 mM | 1.55 mL | 7.75 mL | 15.49 mL |
| 2 mM | 0.77 mL | 3.87 mL | 7.75 mL |
| 10 mM | 0.15 mL | 0.77 mL | 1.55 mL |
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Product Datasheets for HM Janelia Fluor® 526, SE
References for HM Janelia Fluor® 526, SE
References are publications that support the biological activity of the product.
Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787
If you know of a relevant reference for HM Janelia Fluor® 526, SE, please let us know.
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