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Submit ReviewHM Janelia Fluor® 526, NHS ester
Description: Fluorogenic spontaneously blinking yellow-emitting dye (Reactive group: NHS ester). Application: dSTORM, SMLSM, STED, SIM and confocal microscopy
Alternative Names: HM-JF526 NHS
Chemical Name: 2,5-Dioxopyrrolidin-1-yl 3',6'-bis(3,3-difluoroazetidin-1-yl)-2',7'-difluoro-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylate
Purity: ≥90% (HPLC)
Biological Activity for HM Janelia Fluor® 526, NHS ester
Key information: HM Janelia Fluor® 526, NHS ester is a fluorogenic spontaneously blinking yellow-emitting dye; supplied with an NHS ester reactive group for the labeling of primary amines; hydroxymethyl derivative of JF526. Suitable for live cell imaging.
Application: Suitable for confocal microscopy, super-resolution microscopy (SRM) including dSTORM, STED, SIM and single-molecule localization spectroscopy (SMLSM). HM Janelia Fluor® 526, NHS ester is cell permeable.
Properties and Photophysical Data: The spontaneous blinking properties of HM Janelia Fluor® 526, NHS ester avoids the requirement for strongly reducing dSTORM buffers. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 526 nm and 550 nm, respectively.
To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
This product has been recently renamed. The previous name for this product was HM Janelia Fluor® 526, SE.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for HM Janelia Fluor® 526, NHS ester
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 526 nm |
| λem | 550 nm |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Application | dSTORM, SMLSM, STED, SIM, Confocal Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for HM Janelia Fluor® 526, NHS ester
| M. Wt | 645.51 |
| Formula | C31H21F6N3O6 |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 2376841-30-2 |
| InChI Key | HJUSNAUMCROPEK-UHFFFAOYSA-N |
| Smiles | FC1=C(N2CC(F)(C2)F)C=C3OC4=CC(N5CC(F)(C5)F)=C(C=C4C6(C3=C1)OCC7=CC=C(C(ON8C(CCC8=O)=O)=O)C=C67)F |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for HM Janelia Fluor® 526, NHS ester
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 12.91 | 20 |
Preparing Stock Solutions for HM Janelia Fluor® 526, NHS ester
The following data is based on the product molecular weight 645.51. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 7.75 mL | 38.73 mL | 77.46 mL |
| 1 mM | 1.55 mL | 7.75 mL | 15.49 mL |
| 2 mM | 0.77 mL | 3.87 mL | 7.75 mL |
| 10 mM | 0.15 mL | 0.77 mL | 1.55 mL |
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Product Datasheets for HM Janelia Fluor® 526, NHS ester
References for HM Janelia Fluor® 526, NHS ester
References are publications that support the biological activity of the product.
Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787
If you know of a relevant reference for HM Janelia Fluor® 526, NHS ester, please let us know.
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Fluorescent Dyes and Probes Research Product GuideUpdated
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including Janelia Fluor® Dyes
- Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
- Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
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