HM Janelia Fluor® 526, SE

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Description: Fluorogenic spontaneously blinking yellow-emitting dye (Reactive group: NHS ester). Application: dSTORM, SMLSM, STED, SIM and confocal microscopy
Alternative Names: HM-JF526 NHS
Chemical Name: 2,5-Dioxopyrrolidin-1-yl 3',6'-bis(3,3-difluoroazetidin-1-yl)-2',7'-difluoro-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylate
Purity: ≥90% (HPLC)
Literature (1)

Biological Activity for HM Janelia Fluor® 526, SE

Key information: HM Janelia Fluor® 526, SE is a fluorogenic spontaneously blinking yellow-emitting dye; supplied with an NHS ester reactive group for the labeling of primary amines; hydroxymethyl derivative of JF526. Suitable for live cell imaging.

Application: Suitable for confocal microscopy, super-resolution microscopy (SRM) including dSTORM, STED, SIM and single-molecule localization spectroscopy (SMLSM). HM Janelia Fluor® 526, SE is cell permeable.

Properties and Photophysical Data: The spontaneous blinking properties of HM Janelia Fluor® 526, SE avoids the requirement for strongly reducing dSTORM buffers. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 526 nm and 550 nm, respectively.

To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for HM Janelia Fluor® 526, SE

HM Janelia Fluor<sup>®</sup> 526, SE Dye Spectra
Emission Color Yellow
λabs 526 nm
λem 550 nm
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Application dSTORM, SMLSM, STED, SIM, Confocal Microscopy

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Technical Data for HM Janelia Fluor® 526, SE

M. Wt 645.51
Formula C31H21F6N3O6
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 2376841-30-2
Smiles FC1=C(N2CC(F)(C2)F)C=C3OC4=CC(N5CC(F)(C5)F)=C(C=C4C6(C3=C1)OCC7=CC=C(C(ON8C(CCC8=O)=O)=O)C=C67)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for HM Janelia Fluor® 526, SE

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 12.91 20

Preparing Stock Solutions for HM Janelia Fluor® 526, SE

The following data is based on the product molecular weight 645.51. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 7.75 mL 38.73 mL 77.46 mL
1 mM 1.55 mL 7.75 mL 15.49 mL
2 mM 0.77 mL 3.87 mL 7.75 mL
10 mM 0.15 mL 0.77 mL 1.55 mL

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Product Datasheets for HM Janelia Fluor® 526, SE

References for HM Janelia Fluor® 526, SE

References are publications that support the biological activity of the product.

Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787

If you know of a relevant reference for HM Janelia Fluor® 526, SE, please let us know.

Keywords: HM Janelia Fluor 526, SE, HM Janelia Fluor 526, SE supplier, Fluorogenic, Spontaneously, blinking, green, emitting, dyes, for, SMLSM, single-molecule, localisation, localization, spectroscopy, srm, super, resolution, microscopy, STORM, dSTORM, STED, HM-JF526, NHS, Amine, Reactive, Janelia, Fluor, Dyes, 7312, Tocris Bioscience

Citations for HM Janelia Fluor® 526, SE

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