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HM Janelia Fluor® 526, SE
Biological Activity for HM Janelia Fluor® 526, SE
HM Janelia Fluor® 526, SE is a fluorogenic spontaneously blinking green-emitting dye; hydroxymethyl derivative of JF526. Suitable for super-resolution imaging including dSTORM, STED and single-molecule localization spectroscopy (SMLSM). The spontaneous blinking properties of HM Janelia Fluor® 526, avoids the requirement for strongly reducing dSTORM buffers. Also suitable for conventional confocal microscopy and live cell imaging. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®.
To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for HM Janelia Fluor® 526, SE
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Technical Data for HM Janelia Fluor® 526, SE
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for HM Janelia Fluor® 526, SE
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for HM Janelia Fluor® 526, SE
The following data is based on the product molecular weight 645.51. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.2 mM||7.75 mL||38.73 mL||77.46 mL|
|1 mM||1.55 mL||7.75 mL||15.49 mL|
|2 mM||0.77 mL||3.87 mL||7.75 mL|
|10 mM||0.15 mL||0.77 mL||1.55 mL|
Product Datasheets for HM Janelia Fluor® 526, SE
References for HM Janelia Fluor® 526, SE
References are publications that support the biological activity of the product.
Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787
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Fluorescent Probes and Dyes Research Product Guide
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