HM Janelia Fluor® 526, SE

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Cat.No. 7312 - HM Janelia Fluor 526, SE | C31H21F6N3O6 | CAS No. 2376841-30-2
Description: Spontaneously blinking green-emitting dye for SMLSM; λabsλem 526/550 nm
Alternative Names: HM-JF526 NHS
Chemical Name: 2,5-Dioxopyrrolidin-1-yl 3',6'-bis(3,3-difluoroazetidin-1-yl)-2',7'-difluoro-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylate
Purity: ≥90% (HPLC)
Literature (1)

Biological Activity

Spontaneously blinking green-emitting dye; hydroxymethyl derivative of JF526. Suitable for super-resolution imaging including dSTORM, STED and single-molecule localization spectroscopy (SMLSM). The spontaneous blinking properties of HM Janelia Fluor® 526, avoids the requirement for strongly reducing dSTORM buffers. Also suitable for conventional confocal microscopy and live cell imaging.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for HM Janelia Fluor® 526, SE

HM Janelia Fluor<sup>®</sup> 526, SE Dye Spectra
Emission Color Green
λabs 526 nm
λem 550 nm
Quantum Yield (φ) 0.87
Cell Permeable Yes
Application dSTORM, SMLSM, STED, SIM, confocal microscopy

Technical Data

M. Wt 645.51
Formula C31H21F6N3O6
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 2376841-30-2
Smiles FC1=C(N2CC(F)(C2)F)C=C3OC4=CC(N5CC(F)(C5)F)=C(C=C4C6(C3=C1)OCC7=CC=C(C(ON8C(CCC8=O)=O)=O)C=C67)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 12.91 20

Preparing Stock Solutions

The following data is based on the product molecular weight 645.51. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 7.75 mL 38.73 mL 77.46 mL
1 mM 1.55 mL 7.75 mL 15.49 mL
2 mM 0.77 mL 3.87 mL 7.75 mL
10 mM 0.15 mL 0.77 mL 1.55 mL

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References are publications that support the biological activity of the product.

Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787

If you know of a relevant reference for HM Janelia Fluor® 526, SE, please let us know.

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Citations for HM Janelia Fluor® 526, SE

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Literature in this Area

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