You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!Submit Review
HBC 530 New
Mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in live cells. Fluoresces when bound to Pepper aptamer; displays high affinity binding (Kd = 3.5 nM). Suitable for confocal and two-photon microscopy. Confocal excitation maximum = 488 nm, emission maximum= 495 - 595 nm. Two-photon excitation maximum = 976 nm, emission maximum= 475 - 575 nm.
To make a 1000x stock solution, add 200 μL DMSO (Cat. No. 3176).
Optical Data for HBC 530
|Extinction Coefficient (ε)||65300 M-1cm-1|
|Quantum Yield (φ)||0.66|
|Closest Laser line||488 nm|
|Application||Fluorogen, used with RNA light-up aptamer|
|Alternative For||Cat. No. 6434|
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 303.37. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.3 mL||16.48 mL||32.96 mL|
|5 mM||0.66 mL||3.3 mL||6.59 mL|
|10 mM||0.33 mL||1.65 mL||3.3 mL|
|50 mM||0.07 mL||0.33 mL||0.66 mL|
References are publications that support the biological activity of the product.
Chen et al (2019) Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat.Biotechnol. 37 1287 PMID: 31548726
If you know of a relevant reference for HBC 530, please let us know.
View Related Products by Target
Keywords: HBC 530, HBC 530 supplier, HBC530, pepper, aptamer, fluorophore, pepper530, RNA, imaging, live, cells, confocal, light, up, FLAPs, RMFPs, Aptamer-based, Imaging, Fluorescent, Probes, 7277, Tocris Bioscience
Citations for HBC 530
Citations are publications that use Tocris products.
Currently there are no citations for HBC 530. Do you know of a great paper that uses HBC 530 from Tocris? Please let us know.
Reviews for HBC 530
There are currently no reviews for this product. Be the first to review HBC 530 and earn rewards!
Have you used HBC 530?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
New Product Guide [Spring/Summer 2019]
Our new product guide highlights over 215 new products added to the Tocris Bioscience range during the first half of 2019.
- 7-TM Receptors
- Enzyme-Linked Receptors
- Ion Channels
- Nuclear Receptors
- Fluorescent Imaging
- Signal Transduction
- Stem Cells