HBC 530

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Cat.No. 7277 - HBC 530 | C19H17N3O | CAS No. 156840-13-0
Description: GFP fluorophore mimic for imaging RNA in live cells; activated by binding to Pepper aptamers; suitable for confocal and two-photon microscopy
Chemical Name: 4-Cyano-α-[[4-[(2-hydroxyethyl)methylamino]phenyl]methylene]benzeneacetonitrile
Purity: ≥98% (HPLC)
Literature (1)

Biological Activity

Mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in live cells. Fluoresces when bound to Pepper aptamer; displays high affinity binding (Kd = 3.5 nM). Suitable for confocal and two-photon microscopy. Confocal excitation maximum = 488 nm, emission maximum= 495 - 595 nm. Two-photon excitation maximum = 976 nm, emission maximum= 475 - 575 nm.

To make a 1000x stock solution, add 200 μL DMSO (Cat. No. 3176).

Optical Data for HBC 530

λabs 485 nm
λem 530 nm
Extinction Coefficient (ε) 65300 M-1cm-1
Quantum Yield (φ) 0.66
Closest Laser line 488 nm
Application Fluorogen, used with RNA light-up aptamer
Alternative For Cat. No. 6434

Technical Data

M. Wt 303.37
Formula C19H17N3O
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 156840-13-0
Smiles CN(CCO)C1=CC=C(C=C1)/C=C(C#N)/C2=CC=C(C=C2)C#N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 30.34 100

Preparing Stock Solutions

The following data is based on the product molecular weight 303.37. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.3 mL 16.48 mL 32.96 mL
5 mM 0.66 mL 3.3 mL 6.59 mL
10 mM 0.33 mL 1.65 mL 3.3 mL
50 mM 0.07 mL 0.33 mL 0.66 mL

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Product Datasheets

Certificate of Analysis is currently unavailable on-line.
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References are publications that support the biological activity of the product.

Chen et al (2019) Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat.Biotechnol. 37 1287 PMID: 31548726

If you know of a relevant reference for HBC 530, please let us know.

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Citations for HBC 530

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