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HBC 530 New
Mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in live cells. Fluoresces when bound to Pepper aptamer; displays high affinity binding (Kd = 3.5 nM). Suitable for confocal and two-photon microscopy. Confocal excitation maximum = 488 nm, emission maximum= 495 - 595 nm. Two-photon excitation maximum = 976 nm, emission maximum= 475 - 575 nm.
To make a 1000x stock solution, add 200 μL DMSO (Cat. No. 3176).
Optical Data for HBC 530
|Extinction Coefficient (ε)||65300 M-1cm-1|
|Quantum Yield (φ)||0.66|
|Closest Laser line||488 nm|
|Application||Fluorogen, used with RNA light-up aptamer|
|Alternative For||Cat. No. 6434|
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 303.37. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.3 mL||16.48 mL||32.96 mL|
|5 mM||0.66 mL||3.3 mL||6.59 mL|
|10 mM||0.33 mL||1.65 mL||3.3 mL|
|50 mM||0.07 mL||0.33 mL||0.66 mL|
References are publications that support the biological activity of the product.
Chen et al (2019) Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat.Biotechnol. 37 1287 PMID: 31548726
If you know of a relevant reference for HBC 530, please let us know.
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