HBC 530

Pricing Availability   Qty
Description: GFP fluorophore mimic for imaging RNA in live cells; activated by binding to Pepper aptamers; suitable for confocal and two-photon microscopy
Chemical Name: 4-Cyano-α-[[4-[(2-hydroxyethyl)methylamino]phenyl]methylene]benzeneacetonitrile
Purity: ≥98% (HPLC)
Literature (1)

Biological Activity for HBC 530

HBC 530 is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in live cells. Fluoresces when bound to Pepper aptamer; displays high affinity binding (Kd = 3.5 nM). Suitable for confocal and two-photon microscopy. Confocal excitation maximum = 488 nm, emission maximum= 495 - 595 nm. Two-photon excitation maximum = 976 nm, emission maximum= 475 - 575 nm.

To make a 1000x stock solution, add 200 μL DMSO (Cat. No. 3176).

Optical Data for HBC 530

λabs 485 nm
λem 530 nm
Extinction Coefficient (ε) 65300 M-1cm-1
Quantum Yield (φ) 0.66
Closest Laser line 488 nm
Application Fluorogen, used with RNA Light-up Aptamer, Confocal Microscopy
Alternative For Cat. No. 6434

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for HBC 530

M. Wt 303.37
Formula C19H17N3O
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 156840-13-0
Smiles CN(CCO)C1=CC=C(C=C1)/C=C(C#N)/C2=CC=C(C=C2)C#N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for HBC 530

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 30.34 100

Preparing Stock Solutions for HBC 530

The following data is based on the product molecular weight 303.37. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.3 mL 16.48 mL 32.96 mL
5 mM 0.66 mL 3.3 mL 6.59 mL
10 mM 0.33 mL 1.65 mL 3.3 mL
50 mM 0.07 mL 0.33 mL 0.66 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

References for HBC 530

References are publications that support the biological activity of the product.

Chen et al (2019) Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat.Biotechnol. 37 1287 PMID: 31548726

If you know of a relevant reference for HBC 530, please let us know.

Keywords: HBC 530, HBC 530 supplier, HBC530, pepper, aptamer, fluorophore, pepper530, RNA, imaging, live, cells, confocal, light, up, FLAPs, RMFPs, Aptamer-based, Imaging, Fluorescent, Probes, 7277, Tocris Bioscience

Citations for HBC 530

Citations are publications that use Tocris products.

Currently there are no citations for HBC 530. Do you know of a great paper that uses HBC 530 from Tocris? Please let us know.

Reviews for HBC 530

There are currently no reviews for this product. Be the first to review HBC 530 and earn rewards!

Have you used HBC 530?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals