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Description: Na+/2Cl-/K+ (NKCC) symporter inhibitor; also GABAA antagonist
Chemical Name: 5-(Aminosulfonyl)-4-chloro-2-([2-furanylmethyl]amino)benzoic acid
Purity: ≥99% (HPLC)
Citations (6)

Biological Activity for Furosemide

Furosemide is a loop diuretic that inhibits the Na+/2Cl-/K+ (NKCC) symporter. Also acts as a non-competitive antagonist at GABAA receptors with ~ 100-fold greater selectivity for α6-containing receptors than α1-containing receptors.

Compound Libraries for Furosemide

Furosemide is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.

Technical Data for Furosemide

M. Wt 330.74
Formula C12H11ClN2O5S
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 54-31-9
PubChem ID 3440
Smiles ClC1=CC(NCC2=CC=CO2)=C(C(O)=O)C=C1S(=O)(N)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Furosemide

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 33.07 100

Preparing Stock Solutions for Furosemide

The following data is based on the product molecular weight 330.74. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.02 mL 15.12 mL 30.24 mL
5 mM 0.6 mL 3.02 mL 6.05 mL
10 mM 0.3 mL 1.51 mL 3.02 mL
50 mM 0.06 mL 0.3 mL 0.6 mL

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References for Furosemide

References are publications that support the biological activity of the product.

Thompson et al (1999) Residues in transmembrane domains I and II determine γ-aminobutyric acid type AA receptor subtype-selective antagonism by Furos.. Mol.Pharmacol. 55 993 PMID: 10347239

Gutschmidt et al (1999) Anticonvulsant actions of Furos. in vitro. Neuroscience 91 1471 PMID: 10391452

If you know of a relevant reference for Furosemide, please let us know.

View Related Products by Product Action

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Keywords: Furosemide, Furosemide supplier, Na+/2Cl-/K+, NKCC1, NKCC2, cotransporters, inhibitors, inhibits, antagonizes, GABAA, receptor, antagonists, blockers, Sodium, Potassium, Chloride, Ion, Transporters, Pumps, Receptors, diuretics, Na+/K+/Cl-, Symporter, 3109, Tocris Bioscience

6 Citations for Furosemide

Citations are publications that use Tocris products. Selected citations for Furosemide include:

Angelantonio et al (2014) A role for intracellular zinc in glioma alteration of neuronal chloride equilibrium. Cell Death Dis 5 e1501 PMID: 25356870

Choe et al (2015) High salt intake increases blood pressure via BDNF-mediated downregulation of KCC2 and impaired baroreflex inhibition of vasopressin neurons. Autophagy 85 549 PMID: 25619659

Li et al (2017) Artemisinins Target GABAA Receptor Signaling and Impair α Cell Identity. Cell 168 86 PMID: 27916275

Barbaro (2017) Dendritic cell amiloride-sensitive channels mediate sodium-induced inflammation and hypertension. Cell Rep 21 1009 PMID: 29069584

Do you know of a great paper that uses Furosemide from Tocris? Please let us know.

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