FITC-labelled Keap1-Nrf2 probe

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Description: Fluorescent Keap1-Nrf2 peptide
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (3)

Biological Activity for FITC-labelled Keap1-Nrf2 probe

FITC-labelled Keap1-Nrf2 probe is a fluorescent Keap1-Nrf2 peptide (Kd = 22.3 nM). It can be used to assess the binding affinities of Nrf2 peptide inhibitors in Fluorescence Polarization (FP) displacement assay.

Technical Data for FITC-labelled Keap1-Nrf2 probe

M. Wt 1440.5
Formula C67H81N11O23S
Sequence LDEETGEFL

(Modifications: Leu-1 = FITC-Leu, Leu-9 = C-terminal amide)

Storage Store at -20°C
Purity ≥95% (HPLC)
InChI Key RDECLUWFJCNNAY-NZNAQQBOSA-N
Smiles O=C(N[C@@H](CC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H]([C@H](O)C)C(NCC(N[C@@H](CCC(O)=O)C(N[C@@H](CC1=CC=CC=C1)C(N[C@@H](CC(C)C)C(N)=O)=O)=O)=O)=O)=O)=O)=O)[C@@H](NC(NC(C=C2C(O)=O)=CC=C2C(C3=C4C=C(O)C=C3)=C(C=C5)C(O4)=CC5=O)=S)CC(C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for FITC-labelled Keap1-Nrf2 probe

Solubility Soluble to 2 mg/ml in PBS

Product Datasheets for FITC-labelled Keap1-Nrf2 probe

References for FITC-labelled Keap1-Nrf2 probe

References are publications that support the biological activity of the product.

Chen et al (2011) Kinetic analyses of Keap1-Nrf2 interaction and determination of the minimal Nrf2 peptide sequence required for Keap1 binding using surface plasmon resonance. Chem.Biol.Drug Des. 78 1014 PMID: 21920027

Inoyama et al (2012) Optimization of fluorescently labeled Nrf2 peptide probes and the development of a fluorescence polarization assay for the discovery of inhibitors of Keap1-Nrf2 interaction. J.Biomol.Screen 17 435 PMID: 22156223

Wang et al (2020) Development of an enzyme-linked immunosorbent assay for Keap1-Nrf2 interaction inhibitors identification. Redox Biol. 34 101573 PMID: 32422542


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Keywords: FITC-labelled Keap1-Nrf2 probe, FITC-labelled Keap1-Nrf2 probe supplier, Fluorescent, Fluorescence, polarization, FP, displacement, assays, Keap1, Nrf2, ARE, pathway, peptide, transcription, factor, Ubiquitin, E3, Ligases, Enzyme, Probes, and, Substrates, 7627, Tocris Bioscience

Citations for FITC-labelled Keap1-Nrf2 probe

Citations are publications that use Tocris products.

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