FCCP

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Cat.No. 0453 - FCCP | C10H5F3N4O | CAS No. 370-86-5
Description: Oxidative phosphorylation uncoupler
Chemical Name: Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone
Purity: ≥99% (HPLC)
Datasheet
Citations (12)
Reviews (2)

Biological Activity

A very potent uncoupler of oxidative phosphorylation in mitochondria.

Technical Data

M. Wt 254.17
Formula C10H5F3N4O
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 370-86-5
PubChem ID 3330
InChI Key BMZRVOVNUMQTIN-UHFFFAOYSA-N
Smiles N#CC(C#N)=NNC1=CC=C(OC(F)(F)F)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 25.42 100

Preparing Stock Solutions

The following data is based on the product molecular weight 254.17. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.93 mL 19.67 mL 39.34 mL
5 mM 0.79 mL 3.93 mL 7.87 mL
10 mM 0.39 mL 1.97 mL 3.93 mL
50 mM 0.08 mL 0.39 mL 0.79 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References

References are publications that support the biological activity of the product.

Connop et al (1999) Novel effects of FCCP [carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone] on amyloid precursor protein processing. J.Neurochem. 72 1457 PMID: 10098849

Heytler and Pritchard (1962) A new class of uncoupling agents - carbonyl cyanide phenylhydrazones. Biochem.Biophys.Res.Commun. 7 272 PMID: 13907155


If you know of a relevant reference for FCCP, please let us know.

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Keywords: FCCP, FCCP supplier, Oxidative, phosphorylation, uncoupler, Phosphorylation, 0453, Tocris Bioscience

12 Citations for FCCP

Citations are publications that use Tocris products. Selected citations for FCCP include:

Banh et al (2016) PTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxia. Nat Cell Biol 18 803 PMID: 27323329

Mané and Müller (2012) Temporo-spectral imaging of intrinsic optical signals during hypoxia-induced spreading depression-like depolarization. PLoS One 7 e43981 PMID: 22952835

MacDonald et al (2019) A nanoscale, multi-parametric flow cytometry-based platform to study mitochondrial heterogeneity and mitochondrial DNA dynamics. Commun Biol 2 258 PMID: 31312727

Panja et al (2019) Paraneoplastic cerebellar degeneration: Yo antibody alters mitochondrial calcium buffering capacity. Neuropathol Appl Neurobiol 45 141 PMID: 29679372


Do you know of a great paper that uses FCCP from Tocris? Please let us know.

Reviews for FCCP

Average Rating: 4.5 (Based on 2 Reviews.)

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We got expected effect on cells.
By Anonymous on 04/03/2020
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Primary pancreatic ductal epithelial cells

4 μM FCCP

review image

Mitochondrial bioenergetics measurements by reagent screen.
By Anonymous on 03/15/2020
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: mouse brain endothelioma cell line

1 μM

PMID: 27978828
review image