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Description: Inhibits TRPP3-mediated currents; also inhibits the Na+/H+ exchanger (NHE)
Alternative Names: Ethylisopropyl amiloride
Chemical Name: 3-Amino-N-(aminoiminomethyl)-6-chloro-5-[ethyl(1-methylethyl)amino]-2-pyrazinecarboxamide
Purity: ≥98% (HPLC)
Citations (2)
Reviews (1)

Biological Activity for EIPA

EIPA is a TRPP3 channel inhibitor (IC50 = 10.5 μM). Inhibits the Na+/H+ exchanger (NHE). Derivative of amiloride (Cat. No. 0890).

Compound Libraries for EIPA

EIPA is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for EIPA

M. Wt 299.76
Formula C11H18ClN7O
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 1154-25-2
PubChem ID 1795
Smiles ClC1=C(N(C(C)C)CC)N=C(N)C(C(NC(N)=N)=O)=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for EIPA

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 29.98 100

Preparing Stock Solutions for EIPA

The following data is based on the product molecular weight 299.76. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.34 mL 16.68 mL 33.36 mL
5 mM 0.67 mL 3.34 mL 6.67 mL
10 mM 0.33 mL 1.67 mL 3.34 mL
50 mM 0.07 mL 0.33 mL 0.67 mL

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Product Datasheets for EIPA

Certificate of Analysis / Product Datasheet
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References for EIPA

References are publications that support the biological activity of the product.

Dai et al (2007) Inhibition of TRPP3 channel by amil. and analogs. Mol.Pharmacol. 72 1576 PMID: 17804601

Horinouchi et al (2008) Functional role of Na+/H+ exchanger in Ca2+ influx mediated via human endothelin type A receptor stably expressed in Chinese hamster ovary cells. J.Pharm.Sci. 107 456 PMID: 18678984

If you know of a relevant reference for EIPA, please let us know.

View Related Products by Product Action

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Keywords: EIPA, EIPA supplier, sodium, hydrogen, exchangers, NHE-1, NHE, blockers, inhibitors, inhibits, TRPP3, antagonists, Amiloride, ethylisopropyl, amiloride, Ethylisopropyl, Na+/H+, Exchanger, TRPP, 3378, Tocris Bioscience

2 Citations for EIPA

Citations are publications that use Tocris products. Selected citations for EIPA include:

Knudsen et al (2019) Dysregulation of Glucagon Secretion by Hyperglycemia-Induced Sodium-Dependent Reduction of ATP Production. Cell Metab 29 430 PMID: 30415925

Neubert et al (2020) NCX1 Represents an Ionic Na+ Sensing Mechanism in Macrophages. PloS Biol 18 PMID: 32569301

Do you know of a great paper that uses EIPA from Tocris? Please let us know.

Reviews for EIPA

Average Rating: 5 (Based on 1 Review.)

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EIPA (Cat. No. 3378).
By Clara Lubeseder-Martellato on 11/12/2021
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: prmouse pancreatic tumor cell lines

EIPA was added to the cells at 25 uM for 48 hours

PMID: 32434991