dTAG Janelia Fluor® 635

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Description: Fluorogenic srTAG probe for live cell imaging of FBKP12 (F36V/L) labelled proteins.
Chemical Name: 4-((2-(2-(3-((R)-3-(3,4-Dimethoxyphenyl)-1-(((S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carbonyl)oxy)propyl)phenoxy)acetamido)ethyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (1)

Biological Activity for dTAG Janelia Fluor® 635

Key Information: dTAG Janelia Fluor® 635 is a fluorogenic srTAG probe for live cell imaging of FKBP12F36V/L fusion proteins.

Application: Suitable for confocal microscopy and Super Resolution Microscopy (SRM) including STED. dTAG Janelia Fluor® 635 is cell permeable.

Properties and Photophysical Data: In FRAP (fluorescence recovery after photobleaching) experiment, the highest maximum recovery ratio (85%) was shown when U2OS cells expressing FKBPF36L were incubated with 10 μM dTAG Janelia Fluor® 635; excitation and emission maxima (λ) are 640 nm and 665 nm, respectively; quantum yield = 0.41; extinction coefficient = 75,000 M-1cm-1 (in 4% TFA).

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for dTAG Janelia Fluor® 635

Emission Color Red
Brightness
λabs 640 nm
λem 665 nm
Extinction Coefficient (ε) 75000 M-1cm-1
Quantum Yield (φ) 0.41
Closest Laser line 635 nm
Cell Permeable Yes
Application Confocal Microscopy, Super Resolution Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for dTAG Janelia Fluor® 635

M. Wt 1250.18
Formula C69H77F2N5O13Si
Storage Store at -20°C
Purity ≥95% (HPLC)
InChI Key UPUINLLKPHUNRA-QXDPIUJBSA-N
Smiles O=C([C@@H]1CCCCN1C([C@H](C2=CC(OC)=C(OC)C(OC)=C2)CC)=O)O[C@@H](C3=CC(OCC(NCCNC(C4=CC=C(C([O-])=O)C(C(C5=CC=C(N6CC(F)C6)C=C5[Si]7(C)C)=C(C=C/8)C7=CC8=[N+]9CC(F)C\9)=C4)=O)=O)=CC=C3)CCC%10=CC(OC)=C(OC)C=C%10

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for dTAG Janelia Fluor® 635

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 12.5 10

Preparing Stock Solutions for dTAG Janelia Fluor® 635

The following data is based on the product molecular weight 1250.18. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 8 mL 39.99 mL 79.99 mL
0.5 mM 1.6 mL 8 mL 16 mL
1 mM 0.8 mL 4 mL 8 mL
5 mM 0.16 mL 0.8 mL 1.6 mL

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Product Datasheets for dTAG Janelia Fluor® 635

References for dTAG Janelia Fluor® 635

References are publications that support the biological activity of the product.

Du et al (2024) Self-renewable tag for photostable fluorescence imaging of proteins. J.Am.Chem.Soc. 145 18968 PMID: 37596976


If you know of a relevant reference for dTAG Janelia Fluor® 635, please let us know.

Keywords: dTAG Janelia Fluor® 635, dTAG Janelia Fluor® 635 supplier, labeling, probe, FKBP12(F36V), dTAG, fluorescein, fluorescence, super, resolution, microscopy, TAG, Other, Fluorescent, FRAP, SRM, STED, permeable, ZCD, 1, ZCD-1, Probes, Degradation, Platform, 8101, Tocris Bioscience

Citations for dTAG Janelia Fluor® 635

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Currently there are no citations for dTAG Janelia Fluor® 635. Do you know of a great paper that uses dTAG Janelia Fluor® 635 from Tocris? Please let us know.

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