dTAG Janelia Fluor® 635

Pricing Availability   Qty
Description: Fluorogenic srTAG probe for live cell imaging of FBKP12 (F36V/L) labelled proteins.
Chemical Name: 4-((2-(2-(3-((R)-3-(3,4-Dimethoxyphenyl)-1-(((S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carbonyl)oxy)propyl)phenoxy)acetamido)ethyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity for dTAG Janelia Fluor® 635

Key Information: dTAG Janelia Fluor® 635 is a fluorogenic srTAG probe for live cell imaging of FKBP12F36V/L fusion proteins.

Application: Suitable for confocal microscopy and Super Resolution Microscopy (SRM) including STED. dTAG Janelia Fluor® 635 is cell permeable.

Properties and Photophysical Data: In FRAP (fluorescence recovery after photobleaching) experiment, the highest maximum recovery ratio (85%) was shown when U2OS cells expressing FKBPF36L were incubated with 10 μM dTAG Janelia Fluor® 635; excitation and emission maxima (λ) are 640 nm and 665 nm, respectively; quantum yield = 0.41; extinction coefficient = 75,000 M-1cm-1 (in 4% TFA).

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for dTAG Janelia Fluor® 635

Emission Color Red
λabs 640 nm
λem 665 nm
Extinction Coefficient (ε) 75000 M-1cm-1
Quantum Yield (φ) 0.41
Closest Laser line 635 nm
Cell Permeable Yes
Application Confocal Microscopy, Super Resolution Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for dTAG Janelia Fluor® 635

M. Wt 1250.18
Formula C69H77F2N5O13Si
Storage Store at -20°C
Purity ≥95% (HPLC)
Smiles O=C([C@@H]1CCCCN1C([C@H](C2=CC(OC)=C(OC)C(OC)=C2)CC)=O)O[C@@H](C3=CC(OCC(NCCNC(C4=CC=C(C([O-])=O)C(C(C5=CC=C(N6CC(F)C6)C=C5[Si]7(C)C)=C(C=C/8)C7=CC8=[N+]9CC(F)C\9)=C4)=O)=O)=CC=C3)CCC%10=CC(OC)=C(OC)C=C%10

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for dTAG Janelia Fluor® 635

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 12.5 10

Preparing Stock Solutions for dTAG Janelia Fluor® 635

The following data is based on the product molecular weight 1250.18. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 8 mL 39.99 mL 79.99 mL
0.5 mM 1.6 mL 8 mL 16 mL
1 mM 0.8 mL 4 mL 8 mL
5 mM 0.16 mL 0.8 mL 1.6 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for dTAG Janelia Fluor® 635

References for dTAG Janelia Fluor® 635

References are publications that support the biological activity of the product.

Du et al (2024) Self-renewable tag for photostable fluorescence imaging of proteins. J.Am.Chem.Soc. 145 18968 PMID: 37596976

If you know of a relevant reference for dTAG Janelia Fluor® 635, please let us know.

Keywords: dTAG Janelia Fluor® 635, dTAG Janelia Fluor® 635 supplier, labeling, probe, FKBP12(F36V), dTAG, fluorescein, fluorescence, super, resolution, microscopy, TAG, Other, Fluorescent, FRAP, SRM, STED, permeable, ZCD, 1, ZCD-1, Probes, Degradation, Platform, 8101, Tocris Bioscience

Citations for dTAG Janelia Fluor® 635

Citations are publications that use Tocris products.

Currently there are no citations for dTAG Janelia Fluor® 635. Do you know of a great paper that uses dTAG Janelia Fluor® 635 from Tocris? Please let us know.

Reviews for dTAG Janelia Fluor® 635

There are currently no reviews for this product. Be the first to review dTAG Janelia Fluor® 635 and earn rewards!

Have you used dTAG Janelia Fluor® 635?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals