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Description: Potent TLR1/2 agonist
Chemical Name: N-Methyl-4-nitro-2-(4-(4-trifluoromethyl)phenyl)-1H-imidazol-1-yl)aniline
Purity: ≥99% (HPLC)
Reviews (1)
Pathways (1)

Biological Activity for CU-T12-9

CU-T12-9 is a potent TLR1/2 agonist (EC50 = 52.9 nM). Activates the NF-κB pathway and upregulates proinflammatory cytokines in vitro. Also enhances TLR1 and TLR2 dimerization.

Licensing Information

Sold under license from the University of Colorado

Compound Libraries for CU-T12-9

CU-T12-9 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

Technical Data for CU-T12-9

M. Wt 362.31
Formula C17H13F3N4O2
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 1821387-73-8
PubChem ID 91826088
Smiles CNC1=CC=C([N+]([O-])=O)C=C1N2C=C(C3=CC=C(C(F)(F)F)C=C3)N=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for CU-T12-9

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 36.23 100
ethanol 7.25 20

Preparing Stock Solutions for CU-T12-9

The following data is based on the product molecular weight 362.31. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.76 mL 13.8 mL 27.6 mL
5 mM 0.55 mL 2.76 mL 5.52 mL
10 mM 0.28 mL 1.38 mL 2.76 mL
50 mM 0.06 mL 0.28 mL 0.55 mL

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References for CU-T12-9

References are publications that support the biological activity of the product.

Cheng et al (2013) Discovery of small molecule inhibitors of the TLR1-TLR2 complex. Angew.Chem.Int.Ed.Engl. 51 12246 PMID: 22969053

Cheng et al (2015) Specific activation of the TLR1-TLR2 heterodimer by small-molecule agonists Sci.Adv. 1 e1400139 PMID: 26101787

If you know of a relevant reference for CU-T12-9, please let us know.

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Keywords: CU-T12-9, CU-T12-9 supplier, Potent, TLR1/2, activators, activates, Toll-like, Receptors, 5414, Tocris Bioscience

Citations for CU-T12-9

Citations are publications that use Tocris products.

Currently there are no citations for CU-T12-9. Do you know of a great paper that uses CU-T12-9 from Tocris? Please let us know.

Reviews for CU-T12-9

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activation of NF-kappa B pathway in presence of CUt-12-9.
By Tahmineh Safaie on 02/28/2020
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Raw-Blue

Raw-Blue cell lines were treated with different concentration of CU-T129 for 24 hours and the level of Seap was measured

review image

Pathways for CU-T12-9

Toll-like Receptor Signaling Pathway

Toll-like Receptor Signaling Pathway

TLR signaling is involved in the cellular response to threatening molecules such as bacteria and viruses. It results in an inflammatory and immmunological response.