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Click N-Acetylmuramic acid-azide is a derivative of the N-acetylmuramic acid (NAM) component of bacterial peptidoglycans. Incorporated into bacterial peptidoglycans during biosynthesis. Suitable for fluorescent labeling of peptidoglycans when ''click'-conjugated to a fluorescent dye.
Sold under license from the University of Delaware
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
The following data is based on the product molecular weight 334.29. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.05 mM||59.83 mL||299.14 mL||598.28 mL|
|0.25 mM||11.97 mL||59.83 mL||119.66 mL|
|0.5 mM||5.98 mL||29.91 mL||59.83 mL|
|2.5 mM||1.2 mL||5.98 mL||11.97 mL|
References are publications that support the biological activity of the product.
Liang et al (2017) Metabolic labelling of the carbohydrate core in bacterial peptidoglycan and its applications. Nat. Commun. 8 15015 PMID: 28425464
DeMeester et al (2018) Synthesis of functionalized N-acetyl muramic acids to probe bacterial cell wall recycling and biosynthesis. J.Am.Chem.Soc. 140 9458 PMID: 29986130
Wodzanowski et al (2020) Tools for probing host-bacteria interactions in the gut microenvironment: from molecular to cellular levels. Bioorg.Med.Chem.Lett. 30 127116 PMID: 32223923
Wodzanowski et al (2022) Multiscale invasion assay for probing macrophage response to gram-negative bacteria. Front.Chem. 10 842602 PMID: 35242744
Taylor et al (2021e) Localizing peptidoglycan synthesis in helicobacter pylori using clickable metabolic probes. Curr.Protoc. 1 e80 PMID: 33844460
If you know of a relevant reference for Click N-Acetylmuramic acid - azide, please let us know.
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