CI 898 trihydrochloride
Discontinued Product
Unfortunately CI 898 trihydrochloride (Cat. No. 2039) has been withdrawn from sale for commercial reasons.
Biological Activity
Potent lipophilic inhibitor of dihydrofolate reductase (DHFR) that displays antineoplastic and antiprotozoal properties. Inhibits protozoal, bacterial and human DHFR with IC50 values of 1.4, 6.1 and 3.2 nM respectively. Affects G1/S phase cell cycle progression.
Technical Data
| M. Wt | 478.8 |
| Formula | C19H23N5O3.3HCl |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 1658520-97-8 |
| PubChem ID | 45006154 |
| Smiles | NC1=NC(N)=NC(C=C3)=C1C(C)=C3CNC2=CC(OC)=C(OC)C(OC)=C2.Cl.Cl.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Preparing Stock Solutions
The following data is based on the product molecular weight 478.8. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.09 mL | 10.44 mL | 20.89 mL |
| 5 mM | 0.42 mL | 2.09 mL | 4.18 mL |
| 10 mM | 0.21 mL | 1.04 mL | 2.09 mL |
| 50 mM | 0.04 mL | 0.21 mL | 0.42 mL |
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References
References are publications that support the products' biological activity.
Hook et al (1986) Cell cycle effects of trimetrexate (CI-898). Cancer Chemother.Pharmacol. 16 116 PMID: 2936525
Chan and Anderson (2006) Towards species-specific antifolates. Curr.Med.Chem. 13 377 PMID: 16475929
Allegra et al (1987) Potent in vitro and in vivo antitoxoplasma activity of the lipid-soluble antifolate trimetrexate. J.Clin.Invest. 79 478 PMID: 2948969
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Keywords: Dihydrofolate reductases inhibitors inhibits Antineoplastic agent affects G1/S phase progression DHFR Cell Cycle CI898 trihydrochloride TMTX Trimetrexate Dihydrofolate Reductase
1 Citation for CI 898 trihydrochloride
Citations are publications that use Tocris products. Selected citations for CI 898 trihydrochloride include:
Sienkiewicz et al (2008) Chemical and genetic validation of dihydrofolate reductase-thymidylate synthase as a drug target in African trypanosomes. Mol Microbiol 69 520 PMID: 18557814
Literature in this Area
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Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.
- 7-TM Receptors
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- Apoptosis
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