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Biological Activity for CCCP
CCCP is a protonophore. It disrupts mitochondria membrane potential by transporting protons across the mitochondrial inner membrane, which interferes with the proton gradient and ATP synthesis. CCCP is a widely used as an uncoupler of oxidative phosphorylation to induce mitochondrial depolarization.
Technical Data for CCCP
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for CCCP
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for CCCP
The following data is based on the product molecular weight 204.62. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||4.89 mL||24.44 mL||48.87 mL|
|5 mM||0.98 mL||4.89 mL||9.77 mL|
|10 mM||0.49 mL||2.44 mL||4.89 mL|
|50 mM||0.1 mL||0.49 mL||0.98 mL|
References for CCCP
References are publications that support the biological activity of the product.
Heytler and Pritchard (1962) A new class of uncoupling agents - carbonyl cyanide phenylhydrazones. Biochem.Biophys.Res.Commun. 7 272 PMID: 13907155
If you know of a relevant reference for CCCP, please let us know.
Keywords: CCCP, CCCP supplier, Oxidative, phosphorylation, uncoupler, protonophore, proton, gradient, mitochondrial, depolarization, Phosphorylation, 0452, Tocris Bioscience
4 Citations for CCCP
Citations are publications that use Tocris products. Selected citations for CCCP include:
Guo et al (2015) Use of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPSC-CMs) to Monitor Compound Effects on Cardiac Myocyte Signaling Pathways. Curr Protoc Chem Biol 7 141 PMID: 26331525
Choubey et al (2014) BECN1 is involved in the initiation of mitophagy: it facilitates PARK2 translocation to mitochondria. Autophagy 10 1105 PMID: 24879156
Chouhan et al (2010) Presynaptic mitochondria in functionally different motor neurons exhibit similar affinities for Ca2+ but exert little influence as Ca2+ buffers at nerve firing rates in situ. J Neurosci 30 1869 PMID: 20130196
Purnell (2014) Efavirenz induces neuronal autophagy and mitochondrial alterations. J Pharmacol Exp Ther 351 250 PMID: 25161171
Do you know of a great paper that uses CCCP from Tocris? Please let us know.
Reviews for CCCP
Average Rating: 5 (Based on 1 Review.)
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TMRM fluorescence intensity before and after CCCP addition was used for the quantitation of change in mitochondrial membrane potential in spinal cord neurons induced by glutamate receptor agonist AMPA
Time lapse images of TMRM fluorescence of mitochondria rich regions in neurons was recorded. The fluorescence intensity (F) at a given time point was normalized to the initial fluorescence intensity (F0) before addition of the AMPA. At the end of each experiment CCCP (750 nM) was added to the bath. CCCP collapses the mitochondrial membrane potential and decreases the TMRM fluorescence.
Literature in this Area
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