Calyculin A

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Description: Protein phosphatase 1 and 2A inhibitor
Chemical Name: N-[(3S)-[4-(1E)-3-[(2R,3R,4R,7S,8S,9R)-2-[(1S,3S,4S,5R,7E,9E,11E,13Z)-14-Cyano-3,5-dihydroxy-1-methoxy-4,6,8,9,13-pentamethyl-7,9,11,13-tetradecatetraenyl]-9-hydroxy-4,4,8-trimethyl-3-(phosphonooxy)-1,6-dioxaspiro[4.5]dec-7-yl]-1-propenyl]-2-oxazoly]butyl]-4-deoxy-4-(dimethylamino)-5-O-methyl-L-ribonamide
Purity: ≥98% (HPLC)
Datasheet
Citations (9)
Reviews (1)
Literature (1)

Biological Activity for Calyculin A

Calyculin A is a potent and selective cell-permeable inhibitor of protein phosphatase 1 (IC50 = 0.3 - 0.7 nM) and protein phosphatase 2A (IC50 = 0.5 - 1 nM). Displays > 10,000,000-fold selectivity over PP2B and PP2C.

Note: This product is typically prepared in DMSO.

Technical Data for Calyculin A

M. Wt 1009.18
Formula C50H81N4O15P
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 101932-71-2
PubChem ID 3894
InChI Key ULTTYPMRMMDONC-UHFFFAOYSA-N
Smiles O[C@H]1[C@H](C)[C@H](C/C=C/C3=COC([C@@H](C)CCNC([C@H]([C@H]([C@@H](N(C)C)COC)O)O)=O)=N3)O[C@]2([C@](C)(C)[C@@H](OP(O)(O)=O)[C@]([C@@H](OC)C[C@H](O)[C@H](C)[C@H](O)[C@H](C)/C=C(C)/C(C)=C/C=C/C(C)=C\C#N)([H])O2)C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for Calyculin A

Certificate of Analysis / Product Datasheet
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References for Calyculin A

References are publications that support the biological activity of the product.

Favre et al (1997) Differential inhibition and posttranslational modification of protein phosphatase 1 and 2A in MCF7 cells treated with calyculin-A, okadaic acid, and tautomycin. J.Biol.Chem. 272 13856 PMID: 9153244

Ishihara et al (1989) Calyculin A and okadaic acid: inhibitors of protein phosphatase activity. Biochem.Biophys.Res.Commun. 159 871 PMID: 2539153

Lindval et al (1997) The binding mode of calyculin A to protein phosphatase-1. J.Biol.Chem. 272 23312 PMID: 9287341

McCluskey et al (2002) Serine-threonine protein phosphatase inhibitors: development of therapeutic strategies. J.Med.Chem. 45 1151 PMID: 11881984


If you know of a relevant reference for Calyculin A, please let us know.

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Keywords: Calyculin A, Calyculin A supplier, Protein, phosphatase, 1, 2A, inhibitors, inhibits, Calcineurin, Ser/Thr, Phosphatases, PP1, PP2A, CalyculinA, 1336, Tocris Bioscience

9 Citations for Calyculin A

Citations are publications that use Tocris products. Selected citations for Calyculin A include:

Colpaert et al (2005) Nitric oxide relaxes circular smooth muscle of rat distal colon through RhoA/Rho-kinase independent Ca2+ desensitisation. Br J Pharmacol 144 588 PMID: 15655498

Kii et al (2016) Selective inhibition of the kinase DYRK1A by targeting its folding process. Nat Commun 7 11391 PMID: 27102360

Fabbrizi et al (2018) Transient PP2A inhibition alleviates normal tissue stem cell susceptibility to cell death during radiotherapy. Cell Death Dis 9 492 PMID: 29706648

Burdyga et al (2018) Phosphatases control PKA-dependent functional microdomains at the outer mitochondrial membrane. Proc Natl Acad Sci U S A. 115 E6497 PMID: 29941564


Do you know of a great paper that uses Calyculin A from Tocris? Please let us know.

Reviews for Calyculin A

Average Rating: 5 (Based on 1 Review.)

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Product performed ideally over years and would buy again..
By Anonymous on 04/04/2020
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: Sprague-Dawley rats primary neuronal

calyculin A for PP1/PP2A (Cal A, 100 nM)

PMID: 31291571 Reference
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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


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