Bumetanide

Pricing Availability   Qty
Description: Na+/2Cl-/K+ (NKCC) symporter inhibitor
Chemical Name: 3-(Aminosulfonyl)-5-(butylamino)-4-phenoxybenzoic acid
Purity: ≥98% (HPLC)
Datasheet
Citations (12)
Reviews

Biological Activity for Bumetanide

Bumetanide is a loop diuretic that inhibits the Na+/2Cl-/K+ (NKCC) cotransporter. More potent than furosemide (Cat. No. 3109). Bumetanide improves electrophysiological, pathological and cognitive deficits in APOE4 knock-in mice. RNA sequencing of neurons derived from APOE4 induced pluripotent stem cell (iPSC) shows Bumetanide reverses Alzheimer's disease transcriptomic signatures.

Compound Libraries for Bumetanide

Bumetanide is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.

Technical Data for Bumetanide

M. Wt 364.42
Formula C17H20N2O5S
Storage Store at RT
Purity ≥98% (HPLC)
CAS Number 28395-03-1
PubChem ID 2471
InChI Key MAEIEVLCKWDQJH-UHFFFAOYSA-N
Smiles CCCCNC1=CC(C(O)=O)=CC(S(=O)(N)=O)=C1OC2=CC=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Bumetanide

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 36.44 100

Preparing Stock Solutions for Bumetanide

The following data is based on the product molecular weight 364.42. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.74 mL 13.72 mL 27.44 mL
5 mM 0.55 mL 2.74 mL 5.49 mL
10 mM 0.27 mL 1.37 mL 2.74 mL
50 mM 0.05 mL 0.27 mL 0.55 mL

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Product Datasheets for Bumetanide

Certificate of Analysis / Product Datasheet
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References for Bumetanide

References are publications that support the biological activity of the product.

O'Grady et al (1987) Characteristics and functions of Na-K-Cl cotransport in epithelial tissues. Am.J.Physiol. 253 C177 PMID: 3303961

Isenring and Forbush (1997) Ion and bumet. binding by the Na-K-Cl cotransporter. J.Biol.Chem. 272 24556 PMID: 9305921

Morita et al (1999) Functional evidence for involvement of bumetanide-sensitive Na+K+2Cl- cotransport in the hepatoportal Na+ receptor of the sprague-dawley rat. Neurosci.Letts. 264 65 PMID: 10320015

Taubes et al (2021) Experimental and real-world evidence supporting the computational repurposing of bumetanide for APOE4-related Alzheimer's disease. Nature Aging 1 932 PMID: 36172600


If you know of a relevant reference for Bumetanide, please let us know.

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Keywords: Bumetanide, Bumetanide supplier, Na+/2Cl-/K+, NKCC1, NKCC2, cotransporter, inhibitors, inhibits, Sodium, Potassium, Chloride, Cotransporters, Ion, Pumps, APOE4, alzheimer, Alzheimer's, disease, Na+/K+/Cl-, Symporter, 3108, Tocris Bioscience

12 Citations for Bumetanide

Citations are publications that use Tocris products. Selected citations for Bumetanide include:

Babola et al (2020) Purinergic signaling in cochlear supporting cells reduces hair cell excitability by increasing the extracellular space. Elife 9 e52160 PMID: 31913121

Spoljaric et al (2019) KCC2-Mediated Cl- Extrusion Modulates Spontaneous Hippocampal Network Events in Perinatal Rats and Mice. Cell Rep 26 1073 PMID: 30699338

Bhandage et al (2019) Toxoplasma-Induced Hypermigration of Primary Cortical Microglia Implicates GABAergic Signaling. Front Cell Infect Microbiol 9 73 PMID: 30949457

Deeb et al (2013) Disrupted Cl(-) homeostasis contributes to reductions in the inhibitory efficacy of D.pam during hyperexcited states. Eur J Neurosci 38 2453 PMID: 23627375


Do you know of a great paper that uses Bumetanide from Tocris? Please let us know.

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