Bumetanide

Pricing Availability Delivery Time Qty
Cat.No. 3108 - Bumetanide | C17H20N2O5S | CAS No. 28395-03-1
Description: Na+/2Cl-/K+ (NKCC) symporter inhibitor
Chemical Name: 3-(Aminosulfonyl)-5-(butylamino)-4-phenoxybenzoic acid
Purity: ≥99% (HPLC)
Datasheet
Citations (5)
Literature

Biological Activity

Loop diuretic that inhibits the Na+/2Cl-/K+ (NKCC) cotransporter. More potent than furosemide (Cat. No. 3109).

Compound Libraries

Bumetanide is also offered as part of the Tocriscreen Plus and Tocriscreen Library of FDA-Approved Compounds. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 364.42
Formula C17H20N2O5S
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 28395-03-1
PubChem ID 2471
InChI Key MAEIEVLCKWDQJH-UHFFFAOYSA-N
Smiles CCCCNC1=CC(C(O)=O)=CC(S(=O)(N)=O)=C1OC2=CC=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 36.44 100
ethanol 27.33 75

Preparing Stock Solutions

The following data is based on the product molecular weight 364.42. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.74 mL 13.72 mL 27.44 mL
5 mM 0.55 mL 2.74 mL 5.49 mL
10 mM 0.27 mL 1.37 mL 2.74 mL
50 mM 0.05 mL 0.27 mL 0.55 mL

Molarity Calculator

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Reconstitution Calculator

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Dilution Calculator

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

O'Grady et al (1987) Characteristics and functions of Na-K-Cl cotransport in epithelial tissues. Am.J.Physiol. 253 C177 PMID: 3303961

Isenring and Forbush (1997) Ion and bumetanide binding by the Na-K-Cl cotransporter. J.Biol.Chem. 272 24556 PMID: 9305921

Morita et al (1999) Functional evidence for involvement of bumetanide-sensitive Na+K+2Cl- cotransport in the hepatoportal Na+ receptor of the sprague-dawley rat. Neurosci.Letts. 264 65 PMID:


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Keywords: Bumetanide, supplier, Na+/2Cl-/K+, NKCC1, NKCC2, cotransporter, inhibitors, inhibits, Sodium, Potassium, Chloride, Cotransporters, Ion, Pumps, Na+/K+/Cl-, Symporter, Tocris Bioscience

5 Citations for Bumetanide

Citations are publications that use Tocris products. Selected citations for Bumetanide include:

Haam et al (2012) GABA is excitatory in adult vasopressinergic neuroendocrine cells. J Neurosci 32 572 PMID: 22238092

Shulga et al (2012) The loop diuretic bumetanide blocks posttraumatic p75NTR upregulation and rescues injured neurons. J Neurosci 32 1757 PMID: 22302815

Alfonsa et al (2016) Cl- out is a novel cooperative optogenetic tool for extruding chloride from neurons Nature Communications 7 13495 PMID: 27853135

Choe et al (2015) High salt intake increases blood pressure via BDNF-mediated downregulation of KCC2 and impaired baroreflex inhibition of vasopressin neurons. Mol Brain 85 549 PMID: 25619659

Pál et al (2015) Appearance of fast astrocytic component in voltage-sensitive dye imaging of neural activity. Neuron 8 35 PMID: 26043770


Do you know of a great paper that uses Bumetanide from Tocris? If so please let us know.

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Literature in this Area

Cardiovascular

Cardiovascular Research Product Guide

A collection of over 250 products for cardiovascular research, the guide includes research tools for the study of:

  • Hypertension
  • Thrombosis and Hemostasis
  • Atherosclerosis
  • Myocardial Infarction
  • Ischemia/Reperfusion Injury
  • Arrhythmias
  • Heart Failure

Pathways for Bumetanide

Protocols

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