Brefeldin A

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Cat.No. 1231 - Brefeldin A | C16H24O4 | CAS No. 20350-15-6
Description: Disrupts protein translocation to Golgi
Chemical Name: 1,6,7,8,9,11aβ,12,13,14,14αa-Decahydro-1β,13α-dihydroxy-6β-methyl-4H-cyclopent(f)oxacyclotridecin-4-one
Purity: ≥98% (HPLC)
Datasheet
Citations (5)
Reviews
Literature

Biological Activity

Reversible inhibitor of protein translocation from the endoplasmic reticulum (ER) to the Golgi apparatus. Blocks binding of ADP-ribosylation factor to the Golgi apparatus and inhibits GDP-GTP exchange. Can be used to induce autophagy in mammalian cells. Also enhances CRISPR-mediated homology-directed repair (HDR) efficiency ~2-fold when applied at 100 nM, in human induced pluripotent stem cells (iPSCs).

Compound Libraries

Brefeldin A is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 280.36
Formula C16H24O4
Storage Desiccate at -20°C
Purity ≥98% (HPLC)
CAS Number 20350-15-6
PubChem ID 6436187
InChI Key KQNZDYYTLMIZCT-KFKPYADVSA-N
Smiles [H][C@]1(C)CCC\C=C\C2C[C@H](O)C[C@H]2[C@H](O)\C=C\C(=O)O1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
ethanol 2.8 10
DMSO 14.02 50

Preparing Stock Solutions

The following data is based on the product molecular weight 280.36. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.57 mL 17.83 mL 35.67 mL
5 mM 0.71 mL 3.57 mL 7.13 mL
10 mM 0.36 mL 1.78 mL 3.57 mL
50 mM 0.07 mL 0.36 mL 0.71 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the biological activity of the product.

Ktistakis et al (1992) Action of brefeldin A blocked by activation of a pertussis-toxin-sensitive G protein. Nature 356 344 PMID: 1549178

Morinaga et al (1996) Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF)1 and ARF3 that contains a Sec7-like domain. Proc.Natl.Acad.Sci.U.S.A. 93 12856 PMID: 8917509

Tsai et al (1994) Identification of a brefeldin A-insensitive guanine nucleotide-exchange protein for ADP-ribosylation factor in bovine brain. Proc.Natl.Acad.Sci.U.S.A. 91 3063 PMID: 8159707

Ding et al (2007) Differential effects of endoplasmic reticulum stress-induced autophagy on cell survival. J.Biol.Chem. 282 4702 PMID: 17135238

Yu et al (2015) Small molecules enhance CRISPR genome editing in pluripotent stem cells. Cell Stem Cell 16 142 PMID: 25658371


If you know of a relevant reference for Brefeldin A, please let us know.

Keywords: Brefeldin A, Brefeldin A supplier, Disrupts, protein, translocation, Golgi, ADP-ribosylation, factor, BrefeldinA, HDR, homology-directed, repair, CRISPR, cas9, Translocation,, Exocytosis, &, Endocytosis, Autophagy, Reagents, 1231, Tocris Bioscience

5 Citations for Brefeldin A

Citations are publications that use Tocris products. Selected citations for Brefeldin A include:

Halff et al (2014) A novel mechanism for nicotinic potentiation of glutamatergic synapses. J Neurosci 34 2051 PMID: 24501347

Jing et al (2011) Concerted actions of NHERF2 and WNK4 in regulating TRPV5. J Neurosci 404 979 PMID: 21187068

Krokowski (2017) GADD34 function in protein trafficking promotes adaptation to hyperosmotic stress in human corneal cells. Cell Rep 21 2895 PMID: 29212034

Saba et al (2014) Rhinovirus-induced macrophage cytokine expression does not require endocytosis or replication. Am J Respir Cell Mol Biol 50 974 PMID: 24783958

Grassin-Delyle et al (2013) The expression and relaxant effect of bitter taste receptors in human bronchi. Respir Res 14 134 PMID: 24266887


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Literature in this Area

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