Brefeldin A

Pricing Availability   Qty
Description: Disrupts protein translocation to Golgi
Chemical Name: 1,6,7,8,9,11aβ,12,13,14,14aα-Decahydro-1β,13α-dihydroxy-6β-methyl-4H-cyclopent[f]oxacyclotridecin-4-one
Purity: ≥98% (HPLC)
Citations (10)
Reviews (1)

Biological Activity for Brefeldin A

Brefeldin A is a reversible inhibitor of protein translocation from the endoplasmic reticulum (ER) to the Golgi apparatus. Blocks binding of ADP-ribosylation factor (ARF1) to the Golgi apparatus and inhibits GDP-GTP exchange, leading to activation of ER stress signaling pathways. Can be used to induce autophagy in mammalian cells. Also enhances CRISPR-mediated homology-directed repair (HDR) efficiency ~2-fold when applied at 100 nM, in human induced pluripotent stem cells (iPSCs). Antifungal.

Technical Data for Brefeldin A

M. Wt 280.36
Formula C16H24O4
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 20350-15-6
PubChem ID 6436187
Smiles O[C@@H]([C@H]1[C@H](/C=C/CCC[C@H](C)O2)C[C@H](O)C1)/C=C/C2=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Brefeldin A

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 14.02 50

Preparing Stock Solutions for Brefeldin A

The following data is based on the product molecular weight 280.36. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 7.13 mL 35.67 mL 71.34 mL
2.5 mM 1.43 mL 7.13 mL 14.27 mL
5 mM 0.71 mL 3.57 mL 7.13 mL
25 mM 0.14 mL 0.71 mL 1.43 mL

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Product Datasheets for Brefeldin A

Certificate of Analysis / Product Datasheet
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References for Brefeldin A

References are publications that support the biological activity of the product.

Ktistakis et al (1992) Action of brefeldin A blocked by activation of a pertussis-toxin-sensitive G protein. Nature 356 344 PMID: 1549178

Morinaga et al (1996) Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF)1 and ARF3 that contains a Sec7-like domain. Proc.Natl.Acad.Sci.U.S.A. 93 12856 PMID: 8917509

Tsai et al (1994) Identification of a brefeldin A-insensitive guanine nucleotide-exchange protein for ADP-ribosylation factor in bovine brain. Proc.Natl.Acad.Sci.U.S.A. 91 3063 PMID: 8159707

Ding et al (2007) Differential effects of endoplasmic reticulum stress-induced autophagy on cell survival. J.Biol.Chem. 282 4702 PMID: 17135238

Yu et al (2015) Small molecules enhance CRISPR genome editing in pluripotent stem cells. Cell Stem Cell 16 142 PMID: 25658371

Oh-Hashi et al (2021) Elucidation of brefeldin A-induced ER and Golgi stress responses in Neuro2a cells. Mol. Cell. Biochem. 476 3869 PMID: 34129155

If you know of a relevant reference for Brefeldin A, please let us know.

Keywords: Brefeldin A, Brefeldin A supplier, Disrupts, protein, translocation, Golgi, ADP-ribosylation, factor, BrefeldinA, HDR, homology-directed, repair, CRISPR, cas9, ARF1, hematopoietic, haematopoietic, stem, cells, HSC, ER, stress, Autophagy, Reagents, Translocation, Hematopoietic, Stem, Cells, Other, stress/UPR, 1231, Tocris Bioscience

10 Citations for Brefeldin A

Citations are publications that use Tocris products. Selected citations for Brefeldin A include:

Grassin-Delyle et al (2013) The expression and relaxant effect of bitter taste receptors in human bronchi. Respir Res 14 134 PMID: 24266887

Jing et al (2011) Concerted actions of NHERF2 and WNK4 in regulating TRPV5. J Neurosci 404 979 PMID: 21187068

Bowen et al (2017) Golgi-independent secretory trafficking through recycling endosomes in neuronal dendrites and spines. Elife 6 PMID: 28875935

Krokowski (2017) GADD34 function in protein trafficking promotes adaptation to hyperosmotic stress in human corneal cells. Cell Rep 21 2895 PMID: 29212034

Do you know of a great paper that uses Brefeldin A from Tocris? Please let us know.

Reviews for Brefeldin A

Average Rating: 5 (Based on 1 Review.)

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Usage Information.
By Anonymous on 06/20/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: KMS11 or OPM2

0.2 μg/ml

We treated cells for 16 hr.

PMID: 31453810 Reference
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