Brefeldin A

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Cat.No. 1231 - Brefeldin A | C16H24O4 | CAS No. 20350-15-6
Description: Disrupts protein translocation to Golgi
Chemical Name: 1,6,7,8,9,11aβ,12,13,14,14αa-Decahydro-1β,13α-dihydroxy-6β-methyl-4H-cyclopent(f)oxacyclotridecin-4-one
Purity: ≥98% (HPLC)
Datasheet
Citations (10)
Reviews (1)
Literature (1)

Biological Activity

Reversible inhibitor of protein translocation from the endoplasmic reticulum (ER) to the Golgi apparatus. Blocks binding of ADP-ribosylation factor to the Golgi apparatus and inhibits GDP-GTP exchange. Can be used to induce autophagy in mammalian cells. Also enhances CRISPR-mediated homology-directed repair (HDR) efficiency ~2-fold when applied at 100 nM, in human induced pluripotent stem cells (iPSCs). Antifungal.

Technical Data

M. Wt 280.36
Formula C16H24O4
Storage Desiccate at -20°C
Purity ≥98% (HPLC)
CAS Number 20350-15-6
PubChem ID 6436187
InChI Key KQNZDYYTLMIZCT-KFKPYADVSA-N
Smiles [H][C@]1(C)CCC\C=C\C2C[C@H](O)C[C@H]2[C@H](O)\C=C\C(=O)O1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 14.02 50
ethanol 2.8 10

Preparing Stock Solutions

The following data is based on the product molecular weight 280.36. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 7.13 mL 35.67 mL 71.34 mL
2.5 mM 1.43 mL 7.13 mL 14.27 mL
5 mM 0.71 mL 3.57 mL 7.13 mL
25 mM 0.14 mL 0.71 mL 1.43 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References

References are publications that support the biological activity of the product.

Ktistakis et al (1992) Action of brefeldin A blocked by activation of a pertussis-toxin-sensitive G protein. Nature 356 344 PMID: 1549178

Morinaga et al (1996) Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF)1 and ARF3 that contains a Sec7-like domain. Proc.Natl.Acad.Sci.U.S.A. 93 12856 PMID: 8917509

Tsai et al (1994) Identification of a brefeldin A-insensitive guanine nucleotide-exchange protein for ADP-ribosylation factor in bovine brain. Proc.Natl.Acad.Sci.U.S.A. 91 3063 PMID: 8159707

Ding et al (2007) Differential effects of endoplasmic reticulum stress-induced autophagy on cell survival. J.Biol.Chem. 282 4702 PMID: 17135238

Yu et al (2015) Small molecules enhance CRISPR genome editing in pluripotent stem cells. Cell Stem Cell 16 142 PMID: 25658371


If you know of a relevant reference for Brefeldin A, please let us know.

Keywords: Brefeldin A, Brefeldin A supplier, Disrupts, protein, translocation, Golgi, ADP-ribosylation, factor, BrefeldinA, HDR, homology-directed, repair, CRISPR, cas9, Translocation,, Exocytosis, &, Endocytosis, Autophagy, Reagents, Translocation, 1231, Tocris Bioscience

10 Citations for Brefeldin A

Citations are publications that use Tocris products. Selected citations for Brefeldin A include:

Shemorry et al (2019) Caspase-mediated cleavage of IRE1 controls apoptotic cell commitment during endoplasmic reticulum stress. Elife 8 PMID: 31453810

Hossain et al (2018) Hyperactivation of nuclear receptor coactivators induces PERK-dependent cell death. Oncotarget 9 11707 PMID: 29545931

Fauster et al (2018) Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking. Cell Death Differ PMID: 30237509

Nieuwenhuijze et al (2018) Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations. Front Immunol 9 2234 PMID: 30323813


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Reviews for Brefeldin A

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Usage Information.
By Anonymous on 06/20/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: KMS11 or OPM2

0.2 μg/ml

We treated cells for 16 hr.

PMID: 31453810 Reference
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