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Description: Fluorescent probe for nonpolar cavities in proteins; used to detect Aβ fibres
Chemical Name: 4,4'-Bis(phenylamino)-[1,1'-binaphthalene]-5,5'-disulfonic acid dipotassium salt
Purity: ≥97% (HPLC)
Biological Activity for Bis-ANS
Bis-ANS is a fluorescent probe for nonpolar cavities in proteins. Used to detect Aβ fibre (Kd = ~80 nM); exhibits distinct fluorescent profiles for fibres and oligomers. Fluoresces in hydrophobic environments, negligible fluorescence in water solutions. Excitation\emission λ ~ 355\520 nm at pH 7.4. Also modulates liquid-liquid phase separation (LLPS) of TDP-43 low complexitry domain in vitro. Displays biphasic modulation; promotes LLPS at low concentrations but disprut liquid droplets at higher concentrations.
Technical Data for Bis-ANS
| M. Wt | 672.85 |
| Formula | C32H22K2N2O6S2 |
| Storage | Store at RT |
| Purity | ≥97% (HPLC) |
| CAS Number | 65664-81-5 |
| PubChem ID | 16213473 |
| InChI Key | VWLWTJHKQHRTNC-UHFFFAOYSA-L |
| Smiles | O=S(C1=CC=CC2=C1C(NC3=CC=CC=C3)=CC=C2C4=CC=C(NC5=CC=CC=C5)C6=C(S(=O)([O-])=O)C=CC=C46)([O-])=O.[K+].[K+] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Bis-ANS
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 67.28 | 100 | |
| water | 13.46 | 20 |
Preparing Stock Solutions for Bis-ANS
The following data is based on the product molecular weight 672.85. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.49 mL | 7.43 mL | 14.86 mL |
| 5 mM | 0.3 mL | 1.49 mL | 2.97 mL |
| 10 mM | 0.15 mL | 0.74 mL | 1.49 mL |
| 50 mM | 0.03 mL | 0.15 mL | 0.3 mL |
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Product Datasheets for Bis-ANS
References for Bis-ANS
References are publications that support the biological activity of the product.
Rosen et al (1969) Dimer formation from 1-amino-8-naphthalenesulfonate catalyzed by bovine serum albumin. A new fluorescent molecule with exceptional binding properties. Biochemistry 8 3915 PMID: 5388144
Younan et al (2015) A comparison of three fluorophores for the detection of amyloid fibers and prefibrillar oligomeric assemblies. ThT (Thioflavin T); ANS (1-Anilinonaphthalene-8-sulfonic Acid); and bisANS (4,4'-Dianilino-1,1'-binaphthyl-5,5'-disulfonic Acid). Biochemistry 54 4297 PMID: 26087242
Babinchak et al (2020) Small molecules as potent biphasic modulators of protein liquid-liquid phase separation Nat.Commun. 11 5574 PMID: 33149109
If you know of a relevant reference for Bis-ANS, please let us know.
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Keywords: Bis-ANS, Bis-ANS supplier, Amyloid, β, Aβ, peptides, fibers, fibres, oligomers, oligomeric, fluorescent, probe, detects, LLPS, liquid, phase, separation, Beta, Peptides, Fluorescent, B, Probes, 5908, Tocris Bioscience
Citations for Bis-ANS
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Fluorescent Probes and Dyes Research Product Guide
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