Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewBis-ANS
Description: Fluorescent probe for nonpolar cavities in proteins; used to detect Aβ fibres
Chemical Name: 4,4'-Bis(phenylamino)-[1,1'-binaphthalene]-5,5'-disulfonic acid dipotassium salt
Purity: ≥97% (HPLC)
Biological Activity for Bis-ANS
Bis-ANS is a fluorescent probe for nonpolar cavities in proteins. Used to detect Aβ fibre (Kd = ~80 nM); exhibits distinct fluorescent profiles for fibres and oligomers. Fluoresces in hydrophobic environments, negligible fluorescence in water solutions. Excitation\emission λ ~ 355\520 nm at pH 7.4. Also modulates liquid-liquid phase separation (LLPS) of TDP-43 low complexitry domain in vitro. Displays biphasic modulation; promotes LLPS at low concentrations but disprut liquid droplets at higher concentrations.
Technical Data for Bis-ANS
| M. Wt | 672.85 |
| Formula | C32H22K2N2O6S2 |
| Storage | Store at RT |
| Purity | ≥97% (HPLC) |
| CAS Number | 65664-81-5 |
| PubChem ID | 16213473 |
| InChI Key | VWLWTJHKQHRTNC-UHFFFAOYSA-L |
| Smiles | O=S(C1=CC=CC2=C1C(NC3=CC=CC=C3)=CC=C2C4=CC=C(NC5=CC=CC=C5)C6=C(S(=O)([O-])=O)C=CC=C46)([O-])=O.[K+].[K+] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Bis-ANS
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 67.28 | 100 | |
| water | 13.46 | 20 |
Preparing Stock Solutions for Bis-ANS
The following data is based on the product molecular weight 672.85. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.49 mL | 7.43 mL | 14.86 mL |
| 5 mM | 0.3 mL | 1.49 mL | 2.97 mL |
| 10 mM | 0.15 mL | 0.74 mL | 1.49 mL |
| 50 mM | 0.03 mL | 0.15 mL | 0.3 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for Bis-ANS
References for Bis-ANS
References are publications that support the biological activity of the product.
Rosen et al (1969) Dimer formation from 1-amino-8-naphthalenesulfonate catalyzed by bovine serum albumin. A new fluorescent molecule with exceptional binding properties. Biochemistry 8 3915 PMID: 5388144
Younan et al (2015) A comparison of three fluorophores for the detection of amyloid fibers and prefibrillar oligomeric assemblies. ThT (Thioflavin T); ANS (1-Anilinonaphthalene-8-sulfonic Acid); and bisANS (4,4'-Dianilino-1,1'-binaphthyl-5,5'-disulfonic Acid). Biochemistry 54 4297 PMID: 26087242
Babinchak et al (2020) Small molecules as potent biphasic modulators of protein liquid-liquid phase separation Nat.Commun. 11 5574 PMID: 33149109
If you know of a relevant reference for Bis-ANS, please let us know.
View Related Products by Target
Keywords: Bis-ANS, Bis-ANS supplier, Amyloid, β, Aβ, peptides, fibers, fibres, oligomers, oligomeric, fluorescent, probe, detects, LLPS, liquid, phase, separation, Beta, Peptides, Fluorescent, B, Probes, 5908, Tocris Bioscience
Citations for Bis-ANS
Citations are publications that use Tocris products.
Currently there are no citations for Bis-ANS. Do you know of a great paper that uses Bis-ANS from Tocris? Please let us know.
Reviews for Bis-ANS
There are currently no reviews for this product. Be the first to review Bis-ANS and earn rewards!
Have you used Bis-ANS?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Fluorescent Probes and Dyes Research Product Guide
This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes
- Fluorescent Dyes
- Dyes for Flow Cytometry
- Fluorescent Probes
- Anti-fade Reagents
- Bioluminescent Substrates
- Fluorogenic Dyes for Light-Up Aptamers
- Fluorescent Probes for Imaging Bacteria
- TSA Reagents for Enhancing IHC, ICC & FISH Signals