Bis-ANS

Cat. No. 5908 Submit a Review Datasheet / COA / SDS

Description: Fluorescent probe for nonpolar cavities in proteins; used to detect Aβ fibres

Chemical Name: 4,4'-Bis(phenylamino)-[1,1'-binaphthalene]-5,5'-disulfonic acid dipotassium salt

Purity: ≥97% (HPLC)

Datasheet

Citations

Reviews

Literature (1)

Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity

Fluorescent probe for nonpolar cavities in proteins. Used to detect Aβ fibre (K_d = ~80 nM); exhibits distinct fluorescent profiles for fibres and oligomers. Fluoresces in hydrophobic environments, negligible fluorescence in water solutions. Excitation\emission λ ~ 355\520 nm at pH 7.4. Also modulates liquid-liquid phase separation (LLPS) of TDP-43 low complexitry domain in vitro. Displays biphasic modulation; promotes LLPS at low concentrations but disprut liquid droplets at higher concentrations.

Technical Data

M. Wt	672.85
Formula	C₃₂H₂₂K₂N₂O₆S₂
Storage	Store at RT
Purity	≥97% (HPLC)
CAS Number	65664-81-5
PubChem ID	16213473
InChI Key	VWLWTJHKQHRTNC-UHFFFAOYSA-L
Smiles	O=S(C1=CC=CC2=C1C(NC3=CC=CC=C3)=CC=C2C4=CC=C(NC5=CC=CC=C5)C6=C(S(=O)([O-])=O)C=CC=C46)([O-])=O.[K+].[K+]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	67.28	100
	water	13.46	20

Preparing Stock Solutions

The following data is based on the product molecular weight 672.85. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.49 mL	7.43 mL	14.86 mL
5 mM	0.3 mL	1.49 mL	2.97 mL
10 mM	0.15 mL	0.74 mL	1.49 mL
50 mM	0.03 mL	0.15 mL	0.3 mL

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

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Product Datasheets

Certificate of Analysis / Product Datasheet

Safety Datasheet

References

References are publications that support the biological activity of the product.

Rosen et al (1969) Dimer formation from 1-amino-8-naphthalenesulfonate catalyzed by bovine serum albumin. A new fluorescent molecule with exceptional binding properties. Biochemistry 8 3915 PMID: 5388144

Younan et al (2015) A comparison of three fluorophores for the detection of amyloid fibers and prefibrillar oligomeric assemblies. ThT (Thioflavin T); ANS (1-Anilinonaphthalene-8-sulfonic Acid); and bisANS (4,4'-Dianilino-1,1'-binaphthyl-5,5'-disulfonic Acid). Biochemistry 54 4297 PMID: 26087242

Babinchak et al (2020) Small molecules as potent biphasic modulators of protein liquid-liquid phase separation Nat.Commun. 11 5574 PMID: 33149109

If you know of a relevant reference for Bis-ANS, please let us know.

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Citations for Bis-ANS

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Literature in this Area

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