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Cat.No. 5881 - Bicine | C6H13NO4 | CAS No. 150-25-4
Description: General purpose buffer
Chemical Name: N,N-Bis(2-hydroxyethyl)glycine
Purity: ≥99%
Reviews (1)

Biological Activity

Widely used general purpose buffer for biological research. Working pH range in aqueous solution: 7.6-9.0. Used in protein crystallization, ion exchange chromatography and SDS-PAGE.

Technical Data

M. Wt 163.17
Formula C6H13NO4
Storage Store at RT
Purity ≥99%
CAS Number 150-25-4
PubChem ID 8761

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
water 326.34 2000

Preparing Stock Solutions

The following data is based on the product molecular weight 163.17. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
20 mM 0.31 mL 1.53 mL 3.06 mL
100 mM 0.06 mL 0.31 mL 0.61 mL
200 mM 0.03 mL 0.15 mL 0.31 mL
1000 mM 0.01 mL 0.03 mL 0.06 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Keywords: Bicine, Bicine supplier, buffer, protein, crystallization, ion, exchange, chromatography, SDS-PAGE, gel, electrophoresis, Buffers,, Solvents, and, Solutions, 5881, Tocris Bioscience

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Reviews for Bicine

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Used as a buffer for cell culture: Works well.
By Anonymous on 02/11/2019
Species: Human

Bicine was used as a buffer for cell culture. Gives publishable result and performed ideally.

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