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Cat.No. 5620 - BDY FL-X, SE | C24H29BF2N4O5 | CAS No. 217190-09-5
Description: Green BDY (BODIPY® or boron-dipyrromethene) fluorescent dye for the labeling of amines; supplied as NHS ester
Chemical Name: (T-4)-[5-[(3,5-Dimethyl-2H-pyrrol-2-ylidene-&N)methyl]-[N-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1H-pyrrole-2-propanamidato-κN1]difluoroboron
Purity: ≥90% (HPLC)
Protocols (1)
Literature (1)

Biological Activity for BDY FL-X, SE

BDY FL-X, SE is a fluorescent green BDY (BODIPY® or boron-dipyrromethene) dye for the labeling of amines. Exhibits similar excitation and emission spectra to FITC and TFAX 488 dyes. Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a C3 alkyl spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between fluorophore and conjugated biomolecule. The hydrophobic nature of BDY FL makes it ideal for labeling lipids and cell membranes. BDY FL also exhibits narrow emission bandwidths and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Excitation maximum ~504 nM; emission maximum ~510 nM.

BODIPY® is a registered trademark of Molecular Probes.

Optical Data for BDY FL-X, SE

BDY FL-X, SE Dye Spectra
Emission Color Green
λabs 504 nm
λem 510 nm
Extinction Coefficient (ε) 85000 M-1cm-1
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable No
Application Confocal fluorescence p, fluorescence polarization assays and two-photon excitation (TPE) microscopy
Alternative For FAM; Alexa Fluor 488; DyLight 488; Cy2

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for BDY FL-X, SE

M. Wt 502.32
Formula C24H29BF2N4O5
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 217190-09-5
PubChem ID 13007361
Smiles CC(C=C1C)=C2N1[B-](F)(F)[N+]3=C(CCC(NCCCCCC(ON4C(CCC4=O)=O)=O)=O)C=CC3=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for BDY FL-X, SE

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 50.23 100

Preparing Stock Solutions for BDY FL-X, SE

The following data is based on the product molecular weight 502.32. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.99 mL 9.95 mL 19.91 mL
5 mM 0.4 mL 1.99 mL 3.98 mL
10 mM 0.2 mL 1 mL 1.99 mL
50 mM 0.04 mL 0.2 mL 0.4 mL

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References for BDY FL-X, SE

References are publications that support the biological activity of the product.

Baker et al (2010) Influence of fluorophore and linker composition on the pharmacology of fluorescent adenosine A1 receptor ligands. Br.J.Pharmacol. 159 772 PMID: 20105183

If you know of a relevant reference for BDY FL-X, SE, please let us know.

Keywords: BDY FL-X, SE, BDY FL-X, SE supplier, BDYFL-X,SE, fluorescent, reagents, labeling, amines, BDY, Dyes, 5620, Tocris Bioscience

Citations for BDY FL-X, SE

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Protocols for BDY FL-X, SE

The following protocol features additional information for the use of BDY FL-X, SE (Cat. No. 5620).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

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