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BDY FL-X, SE
Fluorescent green BDY (BODIPY® or boron-dipyrromethene) dye for the labeling of amines. Exhibits similar excitation and emission spectra to FITC and TFAX 488 dyes. Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a C3 alkyl spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between fluorophore and conjugated biomolecule. The hydrophobic nature of BDY FL makes it ideal for labeling lipids and cell membranes. BDY FL also exhibits narrow emission bandwidths and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Excitation maximum ~504 nM; emission maximum ~510 nM.
BODIPY® is a registered trademark of Molecular Probes.
Optical Data for BDY FL-X, SE
|Extinction Coefficient (ε)||85000 M-1cm-1|
|Reactive Group||NHS ester|
|Application||Confocal fluorescence p, fluorescence polarization assays and two-photon excitation (TPE) microscopy|
|Alternative For||FAM; Alexa Fluor 488; DyLight 488; Cy2|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectral Viewer
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 502.32. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.99 mL||9.95 mL||19.91 mL|
|5 mM||0.4 mL||1.99 mL||3.98 mL|
|10 mM||0.2 mL||1 mL||1.99 mL|
|50 mM||0.04 mL||0.2 mL||0.4 mL|
References are publications that support the biological activity of the product.
Baker et al (2010) Influence of fluorophore and linker composition on the pharmacology of fluorescent adenosine A1 receptor ligands. Br.J.Pharmacol. 159 772 PMID: 20105183
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Keywords: BDY FL-X, SE, BDY FL-X, SE supplier, BDYFL-X,SE, fluorescent, reagents, labeling, amines, BDY, Dyes, 5620, Tocris Bioscience
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Protocols for BDY FL-X, SE
The following protocol features additional information for the use of BDY FL-X, SE (Cat. No. 5620).
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