TFAX 488, SE

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Cat.No. 6619 - TFAX 488, SE | C25H15N3O13S2Li2 | CAS No. 222164-96-7
Description: Green fluorescent dye; amine reactive
Chemical Name: 3,6-Diamino-9-[2-carboxy-4(5)-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-4,5-disulfoxanthylium bislithium salt
Datasheet
Citations
Reviews
Literature

Biological Activity

Amine reactive green fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Suitable for use in flow cytometry, two-photon excitation microscopy (TPE), and super resolution microscopy techniques, such as dSTORM, SIM and STED. Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1cm-1; quantum yield = 0.92.

Optical Data for TFAX 488, SE

Emission Color Green
Brightness
λabs 495 nm
λem 515 nm
Extinction Coefficient (ε) 73,000 M-1cm-1
Quantum Yield (φ) 0.92
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable No
pH Sensitivity pH insensitive pH 4-10
Application flow cytometry, two-photon excitation microscopy, dSTORM, SIM, STED
Alternative For Alexa Fluor® 488, AF 488, FITC, Cy2

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M. Wt 643.41
Formula C25H15N3O13S2Li2
Storage Store at -20°C
CAS Number 222164-96-7
PubChem ID 23543054
InChI Key FYCGGEVYPRTFDS-UHFFFAOYSA-L
Smiles [NH2+]=C1C=CC2=C(C3=CC=C(C(ON4C(CCC4=O)=O)=O)C=C3C([O-])=O)C5=CC=C(N)C(S(=O)([O-])=O)=C5OC2=C1S(=O)([O-])=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solubility Soluble in DMSO

References

References are publications that support the biological activity of the product.

Rossi et al (2012) Super-resolution imaging of aquaporin-4 orthogonal arrays of particles in cell membranes. J.Cell Sci. 125 4405 PMID: 22718347

Burnette et al (2011) Bleaching/blinking assisted localization microscopy for superresolution imaging using standard fluorescent molecules. Proc.Natl.Acad.Sci.USA. 108 21081 PMID: 22167805

van de Linde et al (2011) Direct stochastic optical reconstruction microscopy with standard fluorescent probes. Nat.Protoc. 6 991 PMID: 21720313

Iwata et al (2005) Increased susceptibility of cytoplasmic over nuclear polyglutamine aggregates to autophagic degradation. Proc.Natl.Acad.Sci.USA. 102 13135 PMID: 16141322

Panchuk-Voloshina et al (1999) Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J.Histochem.Cytochem. 47 1179 PMID: 10449539


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