AZ 20

Pricing Availability   Qty
Description: Potent and selective ATR kinase inhibitor; antitumor
Chemical Name: 4-[4-[(3R)-3-Methyl-4-morpholinyl]-6-[1-(methylsulfonyl)cyclopropyl]-2-pyrimidinyl]-1H-indole
Purity: ≥98% (HPLC)
Citations (8)
Reviews (1)
Literature (2)

Biological Activity for AZ 20

AZ 20 is a potent and selective ATR kinase inhibitor (IC50 = 5 nM). Exhibits 7.6-fold selectivity over mTOR and selectivity over a panel of 442 kinases, including ATM kinase, PI3-K isoforms, and DNA-PK. Inhibits cell growth in cell lines with high baseline levels of replication stress. Displays antitumor effects in vivo.

Licensing Information

Sold for research purposes under agreement from AstraZeneca

Compound Libraries for AZ 20

AZ 20 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for AZ 20

M. Wt 412.51
Formula C21H24N4O3S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1233339-22-4
PubChem ID 46244454
Smiles CS(C4(CC4)C1=CC(N5[C@H](C)COCC5)=NC(C2=CC=CC3=C2C=CN3)=N1)(=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for AZ 20

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 20.63 50
ethanol 4.13 10

Preparing Stock Solutions for AZ 20

The following data is based on the product molecular weight 412.51. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 4.85 mL 24.24 mL 48.48 mL
2.5 mM 0.97 mL 4.85 mL 9.7 mL
5 mM 0.48 mL 2.42 mL 4.85 mL
25 mM 0.1 mL 0.48 mL 0.97 mL

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References for AZ 20

References are publications that support the biological activity of the product.

Foote et al (2013) Discovery of 4-{4-[(3R)-3-Methylmorpholin-4-yl]-6-[1-(methylsulfonyl)cyclopropyl]pyrimidin-2-yl}-1H-indole (AZ20): a potent and selective inhibitor of ATR protein kinase with monotherapy in vivo antitumor activity. J.Med.Chem. 56 2125 PMID: 23394205

If you know of a relevant reference for AZ 20, please let us know.

View Related Products by Product Action

View all ATM and ATR Kinase Inhibitors

Keywords: AZ 20, AZ 20 supplier, AZ20, potent, selective, ATR, Kinase, inhibitors, inhibits, ataxia, telangiectasia, Rad3, related, antitumor, antitumour, replication, stress, ATM, &, Checkpoint, Control, Kinases, 5198, Tocris Bioscience

8 Citations for AZ 20

Citations are publications that use Tocris products. Selected citations for AZ 20 include:

Jiri et al (2020) ATR is essential for preservation of cell mechanics and nuclear integrity during interstitial migration. Nat Commun 11 4828 PMID: 32973141

Skau et al (2016) FMN2 Makes Perinuclear Actin to Protect Nuclei during Confined Migration and Promote Metastasis. Cell 167 1571 PMID: 27839864

Teloni et al (2019) Efficient Pre-mRNA Cleavage Prevents Replication-Stress-Associated Genome Instability. Mol Cell 73 670 PMID: 30639241

Kilic et al (2019) Phase separation of 53BP1 determines liquid-like behavior of DNA repair compartments. EMBO J e101379 PMID: 31267591

Do you know of a great paper that uses AZ 20 from Tocris? Please let us know.

Reviews for AZ 20

Average Rating: 5 (Based on 1 Review.)

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Highly stable.
By Anonymous on 06/22/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: U2OS

AZ20 (2.4 μM)

I used short treatments with AZ20 in the presence of JQ1.

PMID: 30463005
review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Cell Cycle and DNA Damage Research Product Guide

Cell Cycle and DNA Damage Research Product Guide

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Cell Cycle & DNA Damage Repair Poster

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. This poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.