AY 9944 dihydrochloride

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Cat.No. 1639 - AY 9944 dihydrochloride | C22H28Cl2N2.2HCl | CAS No. 366-93-8
Description: Inhibitor of Hedgehog (Hh) signaling. Inhibits Δ7-dehydrocholesterol reductase
Chemical Name: trans-N,N-bis[2-Chlorophenylmethyl]-1,4-cyclohexanedimethanamine dihydrochloride
Purity: ≥99% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Inhibitor of hedgehog (Hh) signaling, possibly via several mechanisms. Inhibits Δ7-dehydrocholesterol reductase (IC50 = 13 nM), thus reduces cholesterol biosynthesis, and also inhibits cholesterol esterification. May also directly block the cellular response to Hh proteins. Teratogenic in vivo.

Technical Data

M. Wt 464.3
Formula C22H28Cl2N2.2HCl
Storage Desiccate at RT
Purity ≥99% (HPLC)
CAS Number 366-93-8
PubChem ID 9954082
InChI Key GZFYZYBWLCYBMI-MYZJJQSMSA-N
Smiles ClC(C=CC=C3)=C3CNC[C@H]1CC[C@H](CNCC2=C(Cl)C=CC=C2)CC1.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 2.32 5
water 23.21 50

Preparing Stock Solutions

The following data is based on the product molecular weight 464.3. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.15 mL 10.77 mL 21.54 mL
5 mM 0.43 mL 2.15 mL 4.31 mL
10 mM 0.22 mL 1.08 mL 2.15 mL
50 mM 0.04 mL 0.22 mL 0.43 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Cooper et al (1998) Teratogen-mediated inhibition of target tissue response to Shh signaling. Science 280 1603 PMID: 9616123

Incardona and Eaton (2000) Cholesterol in signal transduction. Curr.Opin.Cell Biol. 12 193 PMID: 10712926

Kraml et al (1964) Inhibition of the conversion of 7-dehydrocholesterol to cholesterol by AY-9944. Biochem.Biophys.Res.Commun. 15 455 PMID: 4283982

Moebius et al (1998) Molecular cloning and expression of the human Δ7-sterol reductase. Proc.Natl.Acad.Sci.U.S.A. 95 1899 PMID: 9465114


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