Apoptosis Activator 2

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Description: Promotes apoptosome formation and activates caspase-9/caspase-3 pathway. Selectively induces tumor cell apoptosis
Chemical Name: 1-[(3,4-Dichlorophenyl)methyl]-1H-indole-2,3-dione
Purity: ≥99% (HPLC)
Datasheet
Citations (2)
Reviews (1)
Literature (7)

Biological Activity for Apoptosis Activator 2

Apoptosis Activator 2 is an apoptosis activator; promotes the cytochrome c-dependent oligomerization of Apaf-1 into the mature apoptosome. Increases procaspase-9 processing and subsequent caspase-3 activation. Induces apoptosis in tumor cells (IC50 = 4 - 9 μM for leukemia cells) with weak or no effect on normal cell lines or those defective/deficient in Apaf-1, caspase-9 or caspase-3 activity (IC50 > 40 μM).

Technical Data for Apoptosis Activator 2

M. Wt 306.14
Formula C15H9Cl2NO2
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 79183-19-0
PubChem ID 1901244
InChI Key KGRJPLRFGLMQMV-UHFFFAOYSA-N
Smiles ClC1=CC=C(CN2C(=O)C(=O)C3=CC=CC=C23)C=C1Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Apoptosis Activator 2

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
ethanol 1.53 5
DMSO 30.61 100

Preparing Stock Solutions for Apoptosis Activator 2

The following data is based on the product molecular weight 306.14. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.27 mL 16.33 mL 32.66 mL
5 mM 0.65 mL 3.27 mL 6.53 mL
10 mM 0.33 mL 1.63 mL 3.27 mL
50 mM 0.07 mL 0.33 mL 0.65 mL

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Product Datasheets for Apoptosis Activator 2

References for Apoptosis Activator 2

References are publications that support the biological activity of the product.

Alavian et al (2009) Elevtaed p75NTR expression causes death of engrailed-deficient midbrain DArgic neurons by Erk1/2 suppression. Neural Dev. 4 11 PMID: 19291307

Jayaraman and Pike (2009) Progesterone attenuates oestrogen neuroprotection via downregulation of oestrogen receptor expression in cultured neurones. J.Neuroendocrinol. 21 77 PMID: 19094096

Nguyen and Wells (2003) Direct activation of the apoptosis machinery as a mechanism to target cancer cells. Proc.Natl.Acad.Sci.USA 100 7533


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Keywords: Apoptosis Activator 2, Apoptosis Activator 2 supplier, Promotes, apoptosome, formation, activates, caspase-9/caspase-3, pathway, induces, apoptosis, Selective, Inducer, Apoptosis, Activator2, Caspases, Inducers, 2098, Tocris Bioscience

2 Citations for Apoptosis Activator 2

Citations are publications that use Tocris products. Selected citations for Apoptosis Activator 2 include:

Pan et al (2016) SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2. Cell Res 26 190 PMID: 26768768

Stankiewicz et al (2005) Hsp70 inhibits heat-induced apoptosis upstream of mitochondria by preventing Bax translocation. PLoS One 280 38729 PMID: 16172114


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Reviews for Apoptosis Activator 2

Average Rating: 5 (Based on 1 Review.)

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Apoptosis Activator 2 was used to induce apoptosis in epidermal cancer cell line: Works ideally.
By Anonymous on 02/13/2019
Species: Human

Apoptosis Activator 2 was used to induce apoptosis in epidermal cancer cell line.Works great and gives publishable outcome.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


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