Apoptosis Activator 2
Apoptosis activator; promotes the cytochrome c-dependent oligomerization of Apaf-1 into the mature apoptosome. Increases procaspase-9 processing and subsequent caspase-3 activation. Induces apoptosis in tumor cells (IC50 = 4 - 9 μM for leukemia cells) with weak or no effect on normal cell lines or those defective/deficient in Apaf-1, caspase-9 or caspase-3 activity (IC50 > 40 μM).
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 306.14. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.27 mL||16.33 mL||32.66 mL|
|5 mM||0.65 mL||3.27 mL||6.53 mL|
|10 mM||0.33 mL||1.63 mL||3.27 mL|
|50 mM||0.07 mL||0.33 mL||0.65 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Alavian et al (2009) Elevtaed p75NTR expression causes death of engrailed-deficient midbrain dopaminergic neurons by Erk1/2 suppression. Neural Dev. 4 11 PMID: 19291307
Jayaraman and Pike (2009) Progesterone attenuates oestrogen neuroprotection via downregulation of oestrogen receptor expression in cultured neurones. J.Neuroendocrinol. 21 77 PMID: 19094096
If you know of a relevant reference for Apoptosis Activator 2, please let us know.
Keywords: Promotes apoptosome formation activates caspase-9/caspase-3 pathway Selectively induces tumor cell apoptosis Selective Proteinases Proteases Inducer Apoptosis Activator2 Caspases
1 Citation for Apoptosis Activator 2
Citations are publications that use Tocris products. Selected citations for Apoptosis Activator 2 include:
Stankiewicz et al (2005) Hsp70 inhibits heat-induced apoptosis upstream of mitochondria by preventing Bax translocation. PLoS One 280 38729 PMID: 16172114
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