AP 18

Pricing Availability Delivery Time Qty
Cat.No. 3296 - AP 18 | C11H12ClNO | CAS No. 55224-94-7
Description: Reversible TRPA1 channel blocker
Chemical Name: 4-(4-Chlorophenyl)-3-methyl-3-buten-2-one oxime
Purity: ≥98% (HPLC)
Datasheet
Citations (2)
Reviews
Literature

Biological Activity

Reversible TRPA1 channel blocker (IC50 values are 3.1 and 4.5 μM at human and mouse TRPA1 respectively). Blocks cinnameldehyde-induced but not capsaicin-induced nociception and reverses mechanical hyperalgesia in vivo. Also blocks TRPA1 pore dilation (IC50 = 10.3 μM for the inhibition of Yo-Pro uptake).

Compound Libraries

AP 18 is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 209.67
Formula C11H12ClNO
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 55224-94-7
PubChem ID 9584673
InChI Key MHTJEUOFLVQMCL-NJHPPEEMSA-N
Smiles ClC1=CC=C(/C=C(C)/C(C)=N/O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 20.97 100
ethanol 20.97 100

Preparing Stock Solutions

The following data is based on the product molecular weight 209.67. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.77 mL 23.85 mL 47.69 mL
5 mM 0.95 mL 4.77 mL 9.54 mL
10 mM 0.48 mL 2.38 mL 4.77 mL
50 mM 0.1 mL 0.48 mL 0.95 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Certificate of Analysis / Product Datasheet
Select another batch:
Safety Datasheet

References

References are publications that support the biological activity of the product.

Petrus et al (2007) A role of TRPA1 in mechanical hyperalgesia is revealed by pharmacological inhibition. Mol.Pain 3 40 PMID: 18086313

Chen et al (2009) Pore dilation occurs in TRPA1 but not in TRPM8 channels. Mol.Pain 5 3 PMID: 19159452

Taylor-Clark et al (2009) Nitrooleic acid, an endogenous product of nitrative stress, activates nociceptive sensory nerves via the direct activation of TRPA1. Mol.Pharmacol. 75 820 PMID: 19171673


If you know of a relevant reference for AP 18, please let us know.

View Related Products by Target

View Related Products by Product Action

View all TRPA1 Blockers

Keywords: AP 18, AP 18 supplier, Reversible, TRPA1, channel, blockers, Channels, Transient, Receptor, Potential, AP18, 3296, Tocris Bioscience

2 Citations for AP 18

Citations are publications that use Tocris products. Selected citations for AP 18 include:

Capasso et al (2012) Modulation of mouse gastrointestinal motility by allyl isothiocyanate, a constituent of cruciferous vegetables (Brassicaceae): evidence for TRPA1-independent effects. Br J Pharmacol 165 1966 PMID: 21955242

Izzo et al (2012) Inhibitory effect of cannabichromene, a major non-psychotropic cannabinoid extracted from Cannabis sativa, on inflammation-induced hypermotility in mice. Br J Pharmacol 166 1444 PMID: 22300105


Do you know of a great paper that uses AP 18 from Tocris? Please let us know.

Reviews for AP 18

There are currently no reviews for this product. Be the first to review AP 18 and earn rewards!

Have you used AP 18?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Pain

Pain Research Product Guide

A collection of over 250 products for pain research, the guide includes research tools for the study of:

  • Nociception
  • Ion Channels
  • G-Protein-Coupled Receptors
  • Intracellular Signaling
Pain

Pain Poster

Peripheral sensitization is the reduction in the threshold of excitability of sensory neurons that results in an augmented response to a given external stimulus. This poster outlines the excitatory and inhibitory signaling pathways involved in modulation of peripheral sensitization. The role of ion channels, GPCRs, neurotrophins, and cytokines in sensory neurons are also described.