AP 18

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Cat.No. 3296 - AP 18 | C11H12ClNO | CAS No. 55224-94-7
Description: Reversible TRPA1 channel blocker
Chemical Name: 4-(4-Chlorophenyl)-3-methyl-3-buten-2-one oxime
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Reversible TRPA1 channel blocker (IC50 values are 3.1 and 4.5 μM at human and mouse TRPA1 respectively). Blocks cinnameldehyde-induced but not capsaicin-induced nociception and reverses mechanical hyperalgesia in vivo. Also blocks TRPA1 pore dilation (IC50 = 10.3 μM for the inhibition of Yo-Pro uptake).

Compound Libraries

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Technical Data

M. Wt 209.67
Formula C11H12ClNO
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 55224-94-7
PubChem ID 9584673
InChI Key MHTJEUOFLVQMCL-NJHPPEEMSA-N
Smiles ClC1=CC=C(/C=C(C)/C(C)=N/O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 20.97 100
ethanol 20.97 100

Preparing Stock Solutions

The following data is based on the product molecular weight 209.67. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.77 mL 23.85 mL 47.69 mL
5 mM 0.95 mL 4.77 mL 9.54 mL
10 mM 0.48 mL 2.38 mL 4.77 mL
50 mM 0.1 mL 0.48 mL 0.95 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Petrus et al (2007) A role of TRPA1 in mechanical hyperalgesia is revealed by pharmacological inhibition. Mol.Pain 3 40 PMID: 18086313

Chen et al (2009) Pore dilation occurs in TRPA1 but not in TRPM8 channels. Mol.Pain 5 3 PMID: 19159452

Taylor-Clark et al (2009) Nitrooleic acid, an endogenous product of nitrative stress, activates nociceptive sensory nerves via the direct activation of TRPA1. Mol.Pharmacol. 75 820 PMID: 19171673


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Keywords: Reversible TRPA1 channel blockers Channels Transient Receptor Potential AP18 TRPA1

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Literature in this Area

Pain

Pain Research Product Guide

A collection of over 250 products for pain research, the guide includes research tools for the study of:

  • Nociception
  • Ion Channels
  • G-Protein-Coupled Receptors
  • Intracellular Signaling
Pain

Pain Poster

Peripheral sensitization is the reduction in the threshold of excitability of sensory neurons that results in an augmented response to a given external stimulus. This poster outlines the excitatory and inhibitory signaling pathways involved in modulation of peripheral sensitization. The role of ion channels, GPCRs, neurotrophins, and cytokines in sensory neurons are also described.

Pathways for AP 18

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