AP 18

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Description: Reversible TRPA1 channel blocker
Chemical Name: 4-(4-Chlorophenyl)-3-methyl-3-buten-2-one oxime
Purity: ≥98% (HPLC)
Datasheet
Citations (2)
Reviews

Biological Activity for AP 18

AP 18 is a reversible TRPA1 channel blocker (IC50 values are 3.1 and 4.5 μM at human and mouse TRPA1 respectively). Blocks cinnamaldehyde-induced but not capsaicin-induced nociception and reverses mechanical hyperalgesia in vivo. Also blocks TRPA1 pore dilation (IC50 = 10.3 μM for the inhibition of Yo-Pro uptake).

Compound Libraries for AP 18

AP 18 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for AP 18

M. Wt 209.67
Formula C11H12ClNO
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 55224-94-7
PubChem ID 9584673
InChI Key MHTJEUOFLVQMCL-NJHPPEEMSA-N
Smiles ClC1=CC=C(/C=C(C)/C(C)=N/O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for AP 18

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 20.97 100
ethanol 20.97 100

Preparing Stock Solutions for AP 18

The following data is based on the product molecular weight 209.67. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.77 mL 23.85 mL 47.69 mL
5 mM 0.95 mL 4.77 mL 9.54 mL
10 mM 0.48 mL 2.38 mL 4.77 mL
50 mM 0.1 mL 0.48 mL 0.95 mL

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Product Datasheets for AP 18

Certificate of Analysis / Product Datasheet
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References for AP 18

References are publications that support the biological activity of the product.

Petrus et al (2007) A role of TRPA1 in mechanical hyperalgesia is revealed by pharmacological inhibition. Mol.Pain 3 40 PMID: 18086313

Chen et al (2009) Pore dilation occurs in TRPA1 but not in TRPM8 channels. Mol.Pain 5 3 PMID: 19159452

Taylor-Clark et al (2009) Nitrooleic acid, an endogenous product of nitrative stress, activates nociceptive sensory nerves via the direct activation of TRPA1. Mol.Pharmacol. 75 820 PMID: 19171673


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Keywords: AP 18, AP 18 supplier, Reversible, TRPA1, channel, blockers, Channels, Transient, Receptor, Potential, AP18, 3296, Tocris Bioscience

2 Citations for AP 18

Citations are publications that use Tocris products. Selected citations for AP 18 include:

Capasso et al (2012) Modulation of mouse gastrointestinal motility by allyl isothiocyanate, a constituent of cruciferous vegetables (Brassicaceae): evidence for TRPA1-independent effects. Br J Pharmacol 165 1966 PMID: 21955242

Izzo et al (2012) Inhibitory effect of cannabichromene, a major non-psychotropic cannabinoid extracted from Cannabis sativa, on inflammation-induced hypermotility in mice. Br J Pharmacol 166 1444 PMID: 22300105


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