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Endogenous neurotransmitter. Acts at nicotinic and muscarinic acetylcholine receptors.
|Storage||Desiccate at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 181.66. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||5.5 mL||27.52 mL||55.05 mL|
|5 mM||1.1 mL||5.5 mL||11.01 mL|
|10 mM||0.55 mL||2.75 mL||5.5 mL|
|50 mM||0.11 mL||0.55 mL||1.1 mL|
References are publications that support the biological activity of the product.
Wessler et al (1999) The cholinergic 'pitfall': acetylcholine, a universal cell molecule in biological systems, including humans. Clin.Exp.Pharmacol.Physiol. 26 198 PMID: 10081614
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Keywords: Acetylcholine chloride, Acetylcholine chloride supplier, Endogenous, neurotransmitter, Acetylcholine, Nicotinic, Receptors, Non-Selective, Subtypes, nAChR, Muscarinic, (Non-selective), Non-selective, Muscarinics, 2809, Tocris Bioscience
3 Citations for Acetylcholine chloride
Citations are publications that use Tocris products. Selected citations for Acetylcholine chloride include:
Halum et al (2014) Stem cell-derived tissue-engineered constructs for hemilaryngeal reconstruction. Ann Otol Rhinol Laryngol 123 124 PMID: 24574468
Deng et al (2013) Label-free cell phenotypic assessment of the biased agonism and efficacy of agonists at the endogenous muscarinic M3 receptors. J Pharmacol Toxicol Methods 68 323 PMID: 23933114
Fairless et al (2013) Membrane potential measurements of isolated neurons using a voltage-sensitive dye. PLoS One 8 e58260 PMID: 23516458
Do you know of a great paper that uses Acetylcholine chloride from Tocris? Please let us know.
Reviews for Acetylcholine chloride
Average Rating: 5 (Based on 2 Reviews.)
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C57BL6 mice were administered with LPS (5 mg/kg) alone or in combination with Empagliflozin (1 µM) for 16 h. Afterwards mice were sacrificed and aortic rings were isolated. Norepinephrine pre-contracted rings were incubated with indicated (in Image) concentrations of Acetylcholine chloride (ACh) to follow endothelial relaxation.
We used it as an analytical standard for quantification of acetylcholine in brain tissue.
Even 10mM solution does degrades in the -80C freezer. We keep our stock as 100mM and make new every 6 months.
Literature in this Area
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Peripheral sensitization is the reduction in the threshold of excitability of sensory neurons that results in an augmented response to a given external stimulus. This poster outlines the excitatory and inhibitory signaling pathways involved in modulation of peripheral sensitization. The role of ion channels, GPCRs, neurotrophins, and cytokines in sensory neurons are also described.