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ACES

Cat. No. 5879 1 Review Submit a Review Datasheet / COA / SDS
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Cat.No. 5879 - ACES | C4H10N2O4S | CAS No. 7365-82-4
Description: Zwitterionic buffer; used in cell culture and gel eletrophoresis
Chemical Name: N-(2-Acetamido)-2-aminoethanesulfonic acid
Purity: ≥99%
Datasheet
Citations
Reviews (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity

Widely used zwitterionic buffer. Working pH range in aqueous solution: 6.1 - 7.5. Used as a buffereing component in cell culture media, agarose and polyacrylamide eletrophoresis and isoelectric focusing of proteins.

Technical Data

M. Wt 182.2
Formula C4H10N2O4S
Storage Store at RT
Purity ≥99%
CAS Number 7365-82-4
PubChem ID 81832
InChI Key DBXNUXBLKRLWFA-UHFFFAOYSA-N
Smiles NC(CNCCS(=O)(O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 18.22 100

Preparing Stock Solutions

The following data is based on the product molecular weight 182.2. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 5.49 mL 27.44 mL 54.88 mL
5 mM 1.1 mL 5.49 mL 10.98 mL
10 mM 0.55 mL 2.74 mL 5.49 mL
50 mM 0.11 mL 0.55 mL 1.1 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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References

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Keywords: ACES, ACES supplier, buffers, zwitterionic, Goods, cell, culture, gel, eletrophoresis, biochemical, isoeletric, focusing, Buffers,, Solvents, and, Solutions, 5879, Tocris Bioscience

Citations for ACES

Citations are publications that use Tocris products.

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Reviews for ACES

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ACES buffer is an acetamido buffer that is useful for a pH rnge of 6.1 – 7.5, can be used for cell culturing.
By Anonymous on 02/13/2019
Species: Human

ACES buffer is an acetamido buffer that is useful for a pH range of 6.1 – 7.5 and was used for cell culturing.

ACES forms a complex with most common metals and formation constants should be taken into account when using this buffer in a solution containing metal ions. ACES absorbs UV light at 230 nm.

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