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Widely used zwitterionic buffer. Working pH range in aqueous solution: 6.1 - 7.5. Used as a buffereing component in cell culture media, agarose and polyacrylamide eletrophoresis and isoelectric focusing of proteins.
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 182.2. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||5.49 mL||27.44 mL||54.88 mL|
|5 mM||1.1 mL||5.49 mL||10.98 mL|
|10 mM||0.55 mL||2.74 mL||5.49 mL|
|50 mM||0.11 mL||0.55 mL||1.1 mL|
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ACES buffer is an acetamido buffer that is useful for a pH range of 6.1 – 7.5 and was used for cell culturing.
ACES forms a complex with most common metals and formation constants should be taken into account when using this buffer in a solution containing metal ions. ACES absorbs UV light at 230 nm.