A 425619

Pricing Availability   Qty
Cat.No. 5781 - A 425619 | C18H14F3N3O | CAS No. 581809-67-8
Description: Potent TRPV1 antagonist
Chemical Name: N-5-Isoquinolinyl-N'-[[(4-(trifluoromethyl)phenyl]methyl]urea
Purity: ≥98% (HPLC)
Citations (1)
Reviews (1)
Literature (2)

Biological Activity for A 425619

A 425619 is a potent TRPV1 antagonist (IC50 = 3-9 nM). Blocks capsaicin-evoked currents in rat DRG neurons and inhibits TRPV1-activation by anandamide (Cat. No. 1339) and N-arachidonoyl-dopamine. Reduces pain in inflammatory, postoperative and osteoarthritic in vivo pain models.

Compound Libraries for A 425619

A 425619 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for A 425619

M. Wt 345.32
Formula C18H14F3N3O
Storage Store at RT
Purity ≥98% (HPLC)
CAS Number 581809-67-8
PubChem ID 8068410
Smiles O=C(NCC1=CC=C(C(F)(F)F)C=C1)NC2=CC=CC3=CN=CC=C32

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for A 425619

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 34.53 100
ethanol 34.53 100

Preparing Stock Solutions for A 425619

The following data is based on the product molecular weight 345.32. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.9 mL 14.48 mL 28.96 mL
5 mM 0.58 mL 2.9 mL 5.79 mL
10 mM 0.29 mL 1.45 mL 2.9 mL
50 mM 0.06 mL 0.29 mL 0.58 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

References for A 425619

References are publications that support the biological activity of the product.

El Kouhen et al (2005) A-425619 [1-isoquinolin-5-yl-3-(4-trifluoromethyl-benzyl)-urea], a novel and selective transient receptor potential type V1 receptor antagonist, blocks channel activation by vanilloids, heat, and acid. J.Pharmacol.Exp.Ther. 314 400 PMID: 15837819

McGaraughty et al (2006) Systemic and site-specific effects of A-425619, a selective TRPV1 receptor antagonist, on wide dynamic range neurons in CFA-treated and uninjured rats. J.Neurophysiol. 95 18 PMID: 16162831

If you know of a relevant reference for A 425619, please let us know.

View Related Products by Target

View Related Products by Product Action

View all TRPV Channel Antagonists

Keywords: A 425619, A 425619 supplier, A425619, TRPV1, antagonists, antagonism, transient, receptor, potential, channels, vanilloids, pain, nociception, capsaicin, TRPV, 5781, Tocris Bioscience

1 Citation for A 425619

Citations are publications that use Tocris products. Selected citations for A 425619 include:

Chakrabarti et al (2018) Acute inflammation sensitizes knee-innervating sensory neurons and decreases mouse digging behavior in a TRPV1-dependent manner. Neuropharmacology 143 49 PMID: 30240782

Do you know of a great paper that uses A 425619 from Tocris? Please let us know.

Reviews for A 425619

Average Rating: 4 (Based on 1 Review.)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used A 425619?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Inhibiting calcium entry in human mast cells stimulated with cortistatin.
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: LAD2 - mast cells

A425619 product was used to study the role of MrgprX2 receptor in calcium mobilization in human mast cells. At a concentration of 0.01 and 0.03uM A425619 inhibited calcium entry significantly when stimulated mast cells with cortistatin.

PMID: 32038646 Reference
review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Pain Research Product Guide

A collection of over 280 products for pain research, the guide includes research tools for the study of:

  • Nociception
  • Ion Channels
  • G-Protein-Coupled Receptors
  • Intracellular Signaling

Pain Poster

Peripheral sensitization is the reduction in the threshold of excitability of sensory neurons that results in an augmented response to a given external stimulus. This poster outlines the excitatory and inhibitory signaling pathways involved in modulation of peripheral sensitization. The role of ion channels, GPCRs, neurotrophins, and cytokines in sensory neurons are also described.