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5(6)-SFX (Fluorescein), SE
Green fluorescent dye for the labeling of primary amines. Comprising fluorescein, with a commonly used fluorophore, the hexanoic spacer that separates the fluorophore from the molecule to which it is conjugated and makes the NHS ester group more accessible to biopolymers for conjugation. Excitation maximum = 494 nm; emission maximum = 520 nm, extinction coefficient: 74,000 cm-1M-1.
Optical Data for 5(6)-SFX (Fluorescein), SE
|Extinction Coefficient (ε)||70000 M-1cm-1|
|Closest Laser line||488 nm|
|Reactive Group||NHS ester|
|Application||Fluorescence Microscopy, Flow Cytometry, Immunofluorescence Assays (i.e. Western Blot, ELISA, etc)|
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectral Viewer
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 586.55. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.7 mL||8.52 mL||17.05 mL|
|5 mM||0.34 mL||1.7 mL||3.41 mL|
|10 mM||0.17 mL||0.85 mL||1.7 mL|
|50 mM||0.03 mL||0.17 mL||0.34 mL|
References are publications that support the biological activity of the product.
Ito et al (2015) Artificial human Met agonists based on macrocycle scaffolds. Nat.Commun. 6 6373 PMID: 25758345
If you know of a relevant reference for 5(6)-SFX (Fluorescein), SE, please let us know.
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Citations for 5(6)-SFX (Fluorescein), SE
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Protocols for 5(6)-SFX (Fluorescein), SE
The following protocol features additional information for the use of 5(6)-SFX (Fluorescein), SE (Cat. No. 6488).
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