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5(6)-SFX (Fluorescein), SE is a green fluorescent dye for the labeling of primary amines. Comprising fluorescein, with a commonly used fluorophore, the hexanoic spacer that separates the fluorophore from the molecule to which it is conjugated and makes the NHS ester group more accessible to biopolymers for conjugation. Excitation maximum = 494 nm; emission maximum = 520 nm, extinction coefficient: 74,000 cm-1M-1.
Please see the protocol for further information on protein/antibody labeling and conjugation.
|Extinction Coefficient (ε)||70000 M-1cm-1|
|Closest Laser line||488 nm|
|Reactive Group||NHS ester|
|Application||Fluorescence Microscopy, Flow Cytometry, Immunofluorescence Assays (i.e. Western Blot, ELISA, etc)|
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectral Viewer
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
References are publications that support the biological activity of the product.
Ito et al (2015) Artificial human Met agonists based on macrocycle scaffolds. Nat.Commun. 6 6373 PMID: 25758345
Keywords: 5(6)-SFX (Fluorescein), SE, 5(6)-SFX (Fluorescein), SE supplier, Green, fluorescent, reagents, labeling, primary, amines, Fluorescein, dye, Standard, Fluorescein,, Coumarin, and, Rhodamine, Dyes, Flow, Cytometry, 6488, Tocris Bioscience
Citations are publications that use Tocris products.
Currently there are no citations for 5(6)-SFX (Fluorescein), SE.
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The following protocol features additional information for the use of 5(6)-SFX (Fluorescein), SE (Cat. No. 6488).
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our: