5(6)-SFX (Fluorescein), SE

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Cat.No. 6488 - 5(6)-SFX (Fluorescein), SE | C31H26N2O10 | CAS No. 114616-31-8
Description: Green fluorescent dye for the labeling of primary amines
Chemical Name: 6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester
Purity: ≥90% (HPLC)
Datasheet
Citations
Reviews
Protocols (1)
Literature (1)

Biological Activity

Green fluorescent dye for the labeling of primary amines. Comprising fluorescein, with a commonly used fluorophore, the hexanoic spacer that separates the fluorophore from the molecule to which it is conjugated and makes the NHS ester group more accessible to biopolymers for conjugation. Excitation maximum = 494 nm; emission maximum = 520 nm, extinction coefficient: 74,000 cm-1M-1.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

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Technical Data

M. Wt 586.55
Formula C31H26N2O10
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 114616-31-8
PubChem ID 122174028
InChI Key AZTAZTWVNFVDPK-UHFFFAOYSA-N
Smiles OC1=CC2=C(C3(C4=CC=C(C=C4O2)O)OC(C5=C3C=CC=C5)=O)C=C1.O=C(C)NCCCCCC(ON6C(CCC6=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 58.65 100
DMF 58.65 100

Preparing Stock Solutions

The following data is based on the product molecular weight 586.55. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.7 mL 8.52 mL 17.05 mL
5 mM 0.34 mL 1.7 mL 3.41 mL
10 mM 0.17 mL 0.85 mL 1.7 mL
50 mM 0.03 mL 0.17 mL 0.34 mL

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References

References are publications that support the biological activity of the product.

Ito et al (2015) Artificial human Met agonists based on macrocycle scaffolds. Nat.Commun. 6 6373 PMID: 25758345


If you know of a relevant reference for 5(6)-SFX (Fluorescein), SE, please let us know.

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Keywords: 5(6)-SFX (Fluorescein), SE, 5(6)-SFX (Fluorescein), SE supplier, Green, fluorescent, reagents, labeling, primary, amines, Fluorescein, dye, Other, Fluorescent, Dyes, 6488, Tocris Bioscience

Citations for 5(6)-SFX (Fluorescein), SE

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Protocols for 5(6)-SFX (Fluorescein), SE

The following protocol features additional information for the use of 5(6)-SFX (Fluorescein), SE (Cat. No. 6488).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


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