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5(6)-SFX (Fluorescein), SE
Discontinued Product5(6)-SFX (Fluorescein), SE (Cat. No. 6488) has been withdrawn from sale for commercial reasons.
Biological Activity for 5(6)-SFX (Fluorescein), SE
5(6)-SFX (Fluorescein), SE is a green fluorescent dye for the labeling of primary amines. Comprising fluorescein, with a commonly used fluorophore, the hexanoic spacer that separates the fluorophore from the molecule to which it is conjugated and makes the NHS ester group more accessible to biopolymers for conjugation. Excitation maximum = 494 nm; emission maximum = 520 nm, extinction coefficient: 74,000 cm-1M-1.
Please see the protocol for further information on protein/antibody labeling and conjugation.
Optical Data for 5(6)-SFX (Fluorescein), SE
|Extinction Coefficient (ε)||70000 M-1cm-1|
|Closest Laser line||488 nm|
|Reactive Group||NHS ester|
|Application||Fluorescence Microscopy, Flow Cytometry, Immunofluorescence Assays (i.e. Western Blot, ELISA, etc)|
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectral Viewer
Technical Data for 5(6)-SFX (Fluorescein), SE
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for 5(6)-SFX (Fluorescein), SE
References for 5(6)-SFX (Fluorescein), SE
References are publications that support the biological activity of the product.
Ito et al (2015) Artificial human Met agonists based on macrocycle scaffolds. Nat.Commun. 6 6373 PMID: 25758345
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Citations for 5(6)-SFX (Fluorescein), SE
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Protocols for 5(6)-SFX (Fluorescein), SE
The following protocol features additional information for the use of 5(6)-SFX (Fluorescein), SE (Cat. No. 6488).
Literature in this Area
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*Please note that Tocris will only send literature to established scientific business / institute addresses.
Fluorescent Probes and Dyes Research Product Guide
This product guide provides a background to the use of Fluorescent Probes and Dyes, as well as a comprehensive list of our
- Fluorescent Dyes
- Dyes for Flow Cytometry
- Fluorescent Probes
- Anti-fade Reagents
- Bioluminescent Substrates
- Fluorogenic Dyes for Light-Up Aptamers
- Fluorescent Probes for Imaging Bacteria
- TSA Reagents for Enhancing IHC, ICC & FISH Signals