5(6)-SFX (Fluorescein), SE

Discontinued Product

5(6)-SFX (Fluorescein), SE (Cat. No. 6488) has been withdrawn from sale for commercial reasons.
Cat.No. 6488 - 5(6)-SFX (Fluorescein), SE | C31H26N2O10 | CAS No. 114616-31-8
Description: Green fluorescent dye for the labeling of primary amines
Chemical Name: 6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester
Purity: ≥90% (HPLC)
Datasheet
Citations
Reviews
Protocols (1)
Literature (1)

Biological Activity for 5(6)-SFX (Fluorescein), SE

5(6)-SFX (Fluorescein), SE is a green fluorescent dye for the labeling of primary amines. Comprising fluorescein, with a commonly used fluorophore, the hexanoic spacer that separates the fluorophore from the molecule to which it is conjugated and makes the NHS ester group more accessible to biopolymers for conjugation. Excitation maximum = 494 nm; emission maximum = 520 nm, extinction coefficient: 74,000 cm-1M-1.

Please see the protocol for further information on protein/antibody labeling and conjugation.

Optical Data for 5(6)-SFX (Fluorescein), SE

Emission Color Green
λabs 494 nm
λem 518 nm
Extinction Coefficient (ε) 70000 M-1cm-1
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
Application Fluorescence Microscopy, Flow Cytometry, Immunofluorescence Assays (i.e. Western Blot, ELISA, etc)

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for 5(6)-SFX (Fluorescein), SE

M. Wt 586.55
Formula C31H26N2O10
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 114616-31-8
PubChem ID 122174028
InChI Key WAGYELYAQOYZFU-UHFFFAOYSA-N
Smiles OC1=CC2=C(C3(C4=CC=C(C=C4O2)O)OC(C5=C3C=CC(C(NCCCCCC(ON6C(CCC6=O)=O)=O)=O)=C5)=O)C=C1.OC7=CC8=C(C9(C%10=CC=C(C=C%10O8)O)OC(C%11=C9C=C(C(NCCCCCC(ON%12C(CCC%12=O)=O)=O)=O)C=C%11)=O)C=C7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for 5(6)-SFX (Fluorescein), SE

References for 5(6)-SFX (Fluorescein), SE

References are publications that support the biological activity of the product.

Ito et al (2015) Artificial human Met agonists based on macrocycle scaffolds. Nat.Commun. 6 6373 PMID: 25758345

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Citations for 5(6)-SFX (Fluorescein), SE

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Protocols for 5(6)-SFX (Fluorescein), SE

The following protocol features additional information for the use of 5(6)-SFX (Fluorescein), SE (Cat. No. 6488).

Literature in this Area

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Fluorescent Probes and Dyes Research Product Guide

Fluorescent Probes and Dyes Research Product Guide

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