WR 1065 dihydrochloride

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Cat.No. 3356 - WR 1065 dihydrochloride | C5H14N2S.2HCl | CAS No. 14653-77-1
Description: p53 activator. Also ROS scavenger
Chemical Name: 2-[(3-Aminopropyl)amino]ethanethiol dihydrochloride
Datasheet
Citations
Reviews
Literature (3)
Pathways (1)

Biological Activity for WR 1065 dihydrochloride

WR 1065 dihydrochloride is a cell-permeable reactive oxygen species scavenger that activates p53 through a JNK-dependent signaling pathway. Activates p21waf-1 and MDM2, represses transcription of Myc and thymidine kinase, and inhibits DNA topoisomerase IIα. Induces cell cycle arrest and exhibits cytoprotective activity towards normal cells but not cancer cells in vivo. Also exhibits broad spectrum antiviral activity in vitro.

Technical Data for WR 1065 dihydrochloride

M. Wt 207.16
Formula C5H14N2S.2HCl
Storage Desiccate at -20°C
CAS Number 14653-77-1
PubChem ID 146169
InChI Key XDRLRDHLCIFZIW-UHFFFAOYSA-N
Smiles NCCCNCCS.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for WR 1065 dihydrochloride

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 20.72 100
DMSO 20.72 100

Preparing Stock Solutions for WR 1065 dihydrochloride

The following data is based on the product molecular weight 207.16. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.83 mL 24.14 mL 48.27 mL
5 mM 0.97 mL 4.83 mL 9.65 mL
10 mM 0.48 mL 2.41 mL 4.83 mL
50 mM 0.1 mL 0.48 mL 0.97 mL

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Product Datasheets for WR 1065 dihydrochloride

References for WR 1065 dihydrochloride

References are publications that support the biological activity of the product.

North et al (2000) The cytoprotective aminothiol WR1065 activates p21waf-1 and down regulates cell cycle progression through a p53-dependent pathway. Oncogene 19 1206 PMID: 10713709

Pluquet et al (2003) The cytoprotective aminothiol WR1065 activates p53 through a non-genotoxic signaling pathway involving c-Jun N-terminal kinase. J.Biol.Chem. 278 11879 PMID: 12531896

Walker et al (2009) WR1065 mitigates AZT-ddl-induced mutagenesis and inhibits viral replication. Enviorn.Mol.Mutagen. 50 460


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Keywords: WR 1065 dihydrochloride, WR 1065 dihydrochloride supplier, WR1065, dihydrochloride, p53, activators, ROS, scavenger, Apoptosis, Cell, Cycle, Transcription, Factors, reactive, oxygen, species, Non-selective, Antivirals, 3356, Tocris Bioscience

Citations for WR 1065 dihydrochloride

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Literature in this Area

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Cancer Research Product Guide

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

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Cell Cycle and DNA Damage Research Product Guide

Cell Cycle and DNA Damage Research Product Guide

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Cell Cycle & DNA Damage Repair Poster

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.

Pathways for WR 1065 dihydrochloride

p53 Signaling Pathway

p53 Signaling Pathway

p53 signaling plays an important role in the co-ordination of the cellular response different types of stress such as DNA damage and hypoxia. The downstream signals lead to apoptosis, senescence and cell cycle arrest.