Wiskostatin

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Cat.No. 4434 - Wiskostatin | C17H18Br2N2O | CAS No. 253449-04-6
Description: N-WASP inhibitor; inhibits Arp2/3 activation
Chemical Name: 3,6-Dibromo-α-[(dimethylamino)methyl]-9H-cabazole-9-ethanol
Purity: ≥99% (HPLC)
Datasheet
Citations (1)
Literature

Biological Activity

Inhibitor of neural Wiskott-Aldrich syndrome protein (N-WASP) activity. Interacts with the regulatory GTPase-binding domain of N-WASP; inhibits activation of Arp2/3 complex by maintaining N-WASP in an inactive conformation. Also inhibits PIP2-induced actin polymerization (EC50 ~ 4μM).

Compound Libraries

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Technical Data

M. Wt 426.15
Formula C17H18Br2N2O
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 253449-04-6
PubChem ID 2775510
InChI Key XUBJEDZHBUPBKL-UHFFFAOYSA-N
Smiles BrC(C=C3)=CC1=C3N(CC(O)CN(C)C)C2=C1C=C(Br)C=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 42.62 100

Preparing Stock Solutions

The following data is based on the product molecular weight 426.15. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.35 mL 11.73 mL 23.47 mL
5 mM 0.47 mL 2.35 mL 4.69 mL
10 mM 0.23 mL 1.17 mL 2.35 mL
50 mM 0.05 mL 0.23 mL 0.47 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Wegner et al (2008) N-WASP and the Arp2/3 complex are critical regulators of actin in the development of dendritic spines and synapses. J.Biol.Chem. 283 15912 PMID: 18430734

Peterson et al (2004) Chemical inhibition of N-WASP by stabilization of a native autoinhibited conformation. Nat.Struct.Mol.Biol. 11 747 PMID: 15235593

Guerriero and Weisz et al (2006) N-WASP inhibitor wiskostatin nonselectively perturbs membrane transport by decreasing cellular ATP levels. Am.J.Physiol.Cell Physiol. 292 C1562 PMID: 17092993


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View all Actin Inhibitors

Keywords: Arp2/3 complex n-wasp wiskott-aldrich syndrome inhibitors inhibits actin polymerization 3,6-Dibromo-a-[(dimethylamino)methyl]-9H-cabazole-9-ethanol Actin

1 Citation for Wiskostatin

Citations are publications that use Tocris products. Selected citations for Wiskostatin include:

Rassokhin and Pakhomov (2014) Cellular regulation of extension and retraction of pseudopod-like blebs produced by nanosecond pulsed electric field (nsPEF). Cell Biochem Biophys 69 555 PMID: 24488232


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