WAY 213613

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Description: Potent, non-substrate EAAT2 inhibitor
Chemical Name: N-[4-(2-Bromo-4,5-difluorophenoxy)phenyl]-L-asparagine
Purity: ≥99% (HPLC)
Datasheet
Citations (3)
Reviews (1)
Literature (1)

Biological Activity for WAY 213613

WAY 213613 is a potent, non-substrate inhibitor of EAAT2 (GLT-1) that displays > 44-fold selectivity over EAAT1 and EAAT3 (IC50 values are 85, 3787 and 5004 nM for EAAT2, EAAT3 and EAAT1 respectively). Another study has found WAY 213613 has 12 - 26-fold selectivity for EAAT2 over EAAT1, EAAT3 and EAAT4 (IC50 values are 0.071, 0.86, 1.5 and 1.9 nM for EAAT2, EAAT1, EAAT4 and EAAT3, respectively). Exhibits no activity towards ionotropic and metabotropic glutamate receptors.

Compound Libraries for WAY 213613

WAY 213613 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for WAY 213613

M. Wt 415.19
Formula C16H13BrF2N2O4
Storage Desiccate at RT
Purity ≥99% (HPLC)
CAS Number 868359-05-1
PubChem ID 11531745
InChI Key BNYDDAAZMBUFRG-ZDUSSCGKSA-N
Smiles FC1=C(F)C=C(OC2=CC=C(NC(C[C@H](N)C(O)=O)=O)C=C2)C(Br)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for WAY 213613

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
1eq. NaOH 41.51 100
DMSO 41.51 100

Preparing Stock Solutions for WAY 213613

The following data is based on the product molecular weight 415.19. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.41 mL 12.04 mL 24.09 mL
5 mM 0.48 mL 2.41 mL 4.82 mL
10 mM 0.24 mL 1.2 mL 2.41 mL
50 mM 0.05 mL 0.24 mL 0.48 mL

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References for WAY 213613

References are publications that support the biological activity of the product.

Dunlop et al (2005) Characterization of novel aryl-ether, biaryl, and fluorene aspartic acid and diaminopropionic acid analogs as potent inhibitors of the high-affinity glutamate transporter EAAT2. Mol.Pharmacol. 68 974 PMID: 16014807

Beart and Shea (2007) Transporters for L-glutamate: an update on their molecular pharmacology and pathological involvement. Br.J.Pharmacol. 150 5 PMID: 17088867

Fu et al (2018) Chemoenzymatic synthesis and pharmacological characterization of functionalized aspartate analogues as novel excitatory amino acid transporter inhibitors. J.Med.Chem. 61 7741 PMID: 30011368


If you know of a relevant reference for WAY 213613, please let us know.

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View all Glutamate Transporter Inhibitors

Keywords: WAY 213613, WAY 213613 supplier, Potent, non-, EAAT2, inhibitors, inhibits, Excitatory, Amino, Acid, Transporters, GLT-1, Glutamate, Monoamine, Neurotransmitter, WAY213613, 2652, Tocris Bioscience

3 Citations for WAY 213613

Citations are publications that use Tocris products. Selected citations for WAY 213613 include:

Brancaccio et al (2017) Astrocytes Control Circadian Timekeeping in the Suprachiasmatic Nucleus via Glutamatergic Signaling. Neuron 93 1420 PMID: 28285822

Dumont et al (2014) Differential regulation of glutamate transporter subtypes by pro-inflammatory cytokine TNF-α in cortical astrocytes from a rat model of amyotrophic lateral sclerosis. Evid Based Complement Alternat Med 9 e97649 PMID: 24836816

Falcucci et al (2019) Novel positive allosteric modulators of glutamate transport have neuroprotective properties in an in vitro excitotoxic model. ACS Chem Neurosci 10 3437 PMID: 31257852


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Reviews for WAY 213613

Average Rating: 4 (Based on 1 Review.)

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Neuron glia culture.
By Anonymous on 07/12/2020
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Mixed neuron-glia

WAY 213613 (1 μM)

Incubate for 24h


Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


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