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VH 032 New
Biological Activity for VH 032
VH 032 is an inhibitor of E3 ubiquitin ligase VHL (Kd = 185 nM). Blocks interaction between VHL and HIF-α downstream of HIF-α hydroxylation, initiating hypoxic response. VH 032 treatment causes dose dependent upregulation of HIF-target genes including CA9, GLUT1 and PHD2 in different cell lines. Cell permeable.
Technical Data for VH 032
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for VH 032
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for VH 032
The following data is based on the product molecular weight 472.6. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.12 mL||10.58 mL||21.16 mL|
|5 mM||0.42 mL||2.12 mL||4.23 mL|
|10 mM||0.21 mL||1.06 mL||2.12 mL|
|50 mM||0.04 mL||0.21 mL||0.42 mL|
References for VH 032
References are publications that support the biological activity of the product.
Galdeano et al (2014) Structure-guided design and optimization of small molecules targeting the protein-protein interaction between the von Hippel-Lindau (VHL) E3 ubiquitin ligase and the hypoxia inducible factor (HIF) alpha subunit with in vitro nanomolar affinities. J.Med.Chem. 57 8657 PMID: 25166285
Frost et al (2016) Potent and selective chemical probe of hypoxic signalling downstream of HIF-α hydroxylation via VHL inhibition. Nat.Commun. 7 13312 PMID: 27811928
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Keywords: VH 032, VH 032 supplier, VH032, Inhibitors, Inhibition, inhibits, VHL, HIF-alpha, HIF-a, HIF-α, von, Hippel-Lindau, protein, E3, ubiquitin, ligase, Hypoxia, Inducible, Factors, Ubiquitin, Ligases, 7774, Tocris Bioscience
Citations for VH 032
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