TSA Vivid Fluorophore Kit 650

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Cat.No. 7527 - TSA Vivid Fluorophore Kit 650
Description: Signal amplification kit for use in ISH, ICC and IHC
Datasheet
Citations
Reviews
Protocols (1)

Biological Activity for TSA Vivid Fluorophore Kit 650

TSA Vivid reagents are exceptionally bright dyes that offer an effective way to boost signal intensity and detect low-abundance targets in spatial biology applications. They are designed for use in ISH, IHC and ICC experiments where in situ detection of target protein or nucleic acid sequences is required. TSA Vivid reagents have been specifically optimized to deliver exceptional signal-to-noise in the RNAScope™ Multiplex Fluorescent v2 Assay for visualization of gene expression at the single cell level. They are suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining.

The basis for signal amplification comes from the specific and high-density deposition of TSA Vivid dyes adjacent to a horseradish peroxidase (HRP)-labeled probe in a process termed Tyramide Signal Amplification (TSA) or Catalyzed Reporter Deposition (CARD).

Excitation maximum = 654 nm; emission maximum = 668 nm

Please see the product datasheet or protocol for information on product use.

Optical Data for TSA Vivid Fluorophore Kit 650

Spectral data for TSA Vivid Fluorophore kit 650 Emission Color Red
λabs 654 nm
λem 668 nm

Application in RNAscope™ Multiplex Fluorescent v2 Assay

3-plex RNAScope™ Multiplex Fluorescent v2 Assay on HeLa cells

RNAScope™ Multiplex Fluorescent v2 Assay on HeLa cells with the leading competitor dye (left) and TSA Vivid dye 650 (right). Dye used at 1:1500 dilution. Marker shown is UBC in white. Nuclei are counterstained with DAPI (blue, Cat. No. 5748, Tocris).

Technical Data for TSA Vivid Fluorophore Kit 650

Storage Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for TSA Vivid Fluorophore Kit 650

Certificate of Analysis / Product Datasheet
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References for TSA Vivid Fluorophore Kit 650

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Citations for TSA Vivid Fluorophore Kit 650

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Protocols for TSA Vivid Fluorophore Kit 650

The following protocol features additional information for the use of TSA Vivid Fluorophore Kit 650 (Cat. No. 7527).