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Cat.No. 4460 - Trifluorothymidine | C10H11F3N2O5 | CAS No. 70-00-8
Description: Thymidylate synthetase inhibitor
Chemical Name: α,α,α-Trifluorothymidine
Purity: ≥98% (HPLC)
Reviews (1)
Literature (2)
Pathways (1)

Biological Activity

Nucleoside analog; inhibitor of thymidylate synthase. Incorporation of the triphosphate form into DNA induces DNA fragmentation. Exhibits antitumor activity.

Compound Libraries

Trifluorothymidine is also offered as part of the Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 296.2
Formula C10H11F3N2O5
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 70-00-8
PubChem ID 6708818
Smiles O=C1C(C(F)(F)F)=CN(C2O[C@H](CO)[C@@H](O)C2)C(N1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
water 14.81 50
DMSO 29.62 100

Preparing Stock Solutions

The following data is based on the product molecular weight 296.2. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.38 mL 16.88 mL 33.76 mL
5 mM 0.68 mL 3.38 mL 6.75 mL
10 mM 0.34 mL 1.69 mL 3.38 mL
50 mM 0.07 mL 0.34 mL 0.68 mL

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References are publications that support the biological activity of the product.

Okayama et al (2012) Involvement of concentrative nucleoside transporter 1 in intestinal absorption of trifluorothymidine, a novel antitumor nucleoside, in rats. J.Pharmacol.Exp.Ther. 340 457 PMID: 22076553

Emura et al (2005) Potentiation of the antitumor activity of α, α, α-trifluorothymidine by the co-administration of an inhibitor of thymidine phosphorylase at a suitable molar ratio in vivo. Int.J.Oncol. 27 449 PMID: 16010427

Yu et al (2015) Small molecules enhance CRISPR genome editing in pluripotent stem cells. Cell Stem Cell 16 142 PMID: 25658371

If you know of a relevant reference for Trifluorothymidine, please let us know.

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Keywords: Trifluorothymidine, Trifluorothymidine supplier, antitumor, anticancer, nucleoside, analog, thymidylate, synthase, inhibitors, inhibits, DNA,, RNA, and, Protein, Synthesis, Thymidylate, Synthetase, 4460, Tocris Bioscience

Citations for Trifluorothymidine

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Currently there are no citations for Trifluorothymidine. Do you know of a great paper that uses Trifluorothymidine from Tocris? Please let us know.

Reviews for Trifluorothymidine

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Anti-tumor activity of Trifluorothymidine was measured.
By Anonymous on 12/23/2019
Species: Human
Cell Line/Tissue: HeLa human cervical cancer cell line

The HeLa human cervical cancer cell line was cultured in EMEM medium with 10% FCS. Anti-tumor activity of Trifluorothymidine was measured.

The rate of tumor growth (IR) inhibition was regarded as a measure of the antitumor efficacy and was calculated using the relative tumor volume (RTV) in the drug-treated groups (T) compared to that in the control group (C) using the following equation: IR (%) = (1 – T/C) × 100.

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Literature in this Area

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Cancer Metabolism

Cancer Metabolism Poster

Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the main targets for cancer metabolism researchers. Genetic changes and epigenetic modifications in cancer cells alter the regulation of cellular metabolic pathways. These distinct metabolic circuits could provide viable cancer therapeutic targets.

Cell Cycle & DNA Damage Repair

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.

Pathways for Trifluorothymidine


Apoptosis Signaling Pathway

Apoptosis is a physiological process for cell death that is critical during aging and development. It may also be referred to as cell 'suicide'. Apoptosis can be triggered by events both inside and outside of the cell.

p53 Signaling Pathway

p53 signaling plays an important role in the co-ordination of the cellular response different types of stress such as DNA damage and hypoxia. The downstream signals lead to apoptosis, senescence and cell cycle arrest.