Pricing Availability Delivery Time Qty
Cat.No. 4460 - Trifluorothymidine | C10H11F3N2O5 | CAS No. 70-00-8
Description: Thymidylate synthase inhibitor; induces DNA fragmentation
Chemical Name: α,α,α-Trifluorothymidine
Purity: ≥98% (HPLC)

Biological Activity

Nucleoside analog; inhibitor of thymidylate synthase. Incorporation of the triphosphate form into DNA induces DNA fragmentation. Exhibits antitumor activity.

Compound Libraries

Trifluorothymidine is also offered as part of the Tocriscreen Plus and Tocriscreen Library of FDA-Approved Compounds. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 296.2
Formula C10H11F3N2O5
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 70-00-8
PubChem ID 6708818
Smiles O=C1C(C(F)(F)F)=CN(C2O[C@H](CO)[C@@H](O)C2)C(N1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 29.62 100
water 14.81 50

Preparing Stock Solutions

The following data is based on the product molecular weight 296.2. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.38 mL 16.88 mL 33.76 mL
5 mM 0.68 mL 3.38 mL 6.75 mL
10 mM 0.34 mL 1.69 mL 3.38 mL
50 mM 0.07 mL 0.34 mL 0.68 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets

Safety Datasheet


References are publications that support the products' biological activity.

Okayama et al (2012) Involvement of concentrative nucleoside transporter 1 in intestinal absorption of trifluorothymidine, a novel antitumor nucleoside, in rats. J.Pharmacol.Exp.Ther. 340 457 PMID: 22076553

Emura et al (2005) Potentiation of the antitumor activity of α, α, α-trifluorothymidine by the co-administration of an inhibitor of thymidine phosphorylase at a suitable molar ratio in vivo. Int.J.Oncol. 27 449 PMID: 16010427

Yu et al (2015) Small molecules enhance CRISPR genome editing in pluripotent stem cells. Cell Stem Cell 16 142 PMID: 25658371

If you know of a relevant reference for Trifluorothymidine, please let us know.

View Related Products by Product Action

View all Other Synthase/Synthetase Inhibitors

Keywords: Trifluorothymidine, supplier, antitumor, anticancer, nucleoside, analog, thymidylate, synthase, inhibitors, inhibits, Other, Synthases/Synthetases, DNA,, RNA, and, Protein, Synthesis, Other, Synthases/Synthetases, Tocris Bioscience

Citations for Trifluorothymidine

Citations are publications that use Tocris products.

Currently there are no citations for Trifluorothymidine. Do you know of a great paper that uses Trifluorothymidine from Tocris? If so please let us know.

Commented out for usability testing


TODO: Add Reviews

Literature in this Area


Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis

Pathways for Trifluorothymidine


TODO: Add Protocols