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Biological Activity for Tissue Clearing Pro Reagent 2
Tissue Clearing Pro is a solvent-based tissue clearing technique, comparable to BABB and DISCO. It does not cause significant shrinkage or tissue damage and does not use as harsh a solvent as other solvent-based techniques. Tissue Clearing Pro Reagent 2 is part of our Tissue Clearing Pro kit (Cat. No. 7389) that can clear a whole mouse brain of up to 8 mm thickness in 24 hours; for sections with a thickness of 1 mm this can be achieved in 2 hours. For small tissues and thin brain slices (500 μm) that do not require permeabilization, only Tissue Clearing Pro Reagent 1 (Cat. No. 7563) is required and tissues can be imaged in Tissue Clearing Pro Reagent 1. Larger tissues must be placed in Tissue Clearing Pro Reagent 2 to finish clearing, then these samples can be mounted and imaged in Tissue Clearing Pro Reagent 2. This product is compatible with fluorescent protein, immunolabeling and small molecule staining techniques, as well as being suitable for use with all standard dyes and buffers. Following 3D imaging, clearing can be reversed for follow-up 2D histology staining. Tissue Clearing Pro Reagent 2 has a refractive index of 1.53. Supplied as 1 x 30 mL vial.
Please read the Tissue Clearing Pro Guidebook for more information.
Technical Data for Tissue Clearing Pro Reagent 2
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Tissue Clearing Pro Reagent 2
References for Tissue Clearing Pro Reagent 2
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals