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Biological Activity for Tissue Clearing Pro
Tissue Clearing Pro is a rapid, easy-to-use, reversible and non-destructive clearing and staining kit, which allows effective tissue clearing and staining of whole tissues. This kit can clear a whole mouse brain of up to 8 mm thickness in 24 hours; for sections with a thickness of 1 mm this can be achieved in 2 hours. Tissue Clearing Pro is compatible with fluorescent protein, immunolabeling and small molecule staining techniques, as well as being suitable for use with all standard dyes and buffers. Following 3D imaging, Tissue Clearing Pro can be reversed for follow-up 2D histology staining. The kit comes with a getting started guide and a more comprehensive tissue clearing guidebook, providing detailed protocols. The Tissue Clearing Pro kit contains 30 mL of Tissue Clearing Pro clearing reagent 1 and 2, plus the 5 buffer solutions required to process tissues for immunolabeling, and is enough to process four whole mouse brains (approx. 2 g of tissue).
A wide range of IHC validated antibodies are available from R&D Systems and Novus Biologicals; optimization of antibody incubation conditions for use with the Tissue Clearing Pro technique is recommended.
Please read the Tissue Clearing Pro guidebook for more information and detailed protocols for using this kit.
Guidebook and Protocol for Tissue Clearing Pro
For information on how to use Tissue Clearing Pro download our Guidebook.
Technical Data for Tissue Clearing Pro
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Tissue Clearing Pro
References for Tissue Clearing Pro
References are publications that support the biological activity of the product.
Sargent (2019) Clarification and confocal imaging of the nonhuman primate placental micro-anatomy. Biotechniques 66 73 PMID: 30370778
Regla-Nava et al (2019) Detection of Zika virus in mouse mammary gland and breast milk. PLoS Negl.Trop.Dis. 13 e0007080 PMID: 30742628
Robino et al (2020) Spatial and biochemical interactions between bone marrow adipose tissue and hematopoietic stem and progenitor cells in rhesus macaques. Bone 133 115248 PMID: 31972314
Choi et al (2020) Innate lymphoid cells play a pathogenic role in pericarditis. Cell Rep. 30 2989 PMID: 32130902
If you know of a relevant reference for Tissue Clearing Pro, please let us know.
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Citations for Tissue Clearing Pro
Citations are publications that use Tocris products.
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Literature in this Area
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Fluorescent Probes and Dyes Research Product Guide
This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes
- Fluorescent Dyes
- Dyes for Flow Cytometry
- Fluorescent Probes
- Anti-fade Reagents
- Bioluminescent Substrates
- Fluorogenic Dyes for Light-Up Aptamers
- Fluorescent Probes for Imaging Bacteria
- TSA Reagents for Enhancing IHC, ICC & FISH Signals