TFB-TBOA

Pricing Availability Delivery Time Qty
Cat.No. 2532 - TFB-TBOA | C19H17F3N2O6 | CAS No. 480439-73-4
Description: High affinity EAAT1 and EAAT2 blocker
Chemical Name: (3S)-3-[[3-[[4-(Trifluoromethyl)benzoyl]amino]phenyl]methoxy]-L-aspartic acid
Purity: ≥98% (HPLC)
Datasheet
Citations (7)
Literature

Biological Activity

Potent and selective glial glutamate transporter EAAT1 and EAAT2 inhibitor (IC50 values are 17, 22 and 300 nM for EAAT2, EAAT1 and EAAT3 respectively). Has no effect on EAAT4 and EAAT5, or a wide range of neuronal receptors and transporters. Attenuates glutamate-stimulated intracellular Na+ elevation in astrocytes in vitro (IC50 = 43 nM). Induces severe convulsions in vivo.

Licensing Information

Sold for research purposes under license from Suntory Ltd.

Technical Data

M. Wt 426.35
Formula C19H17F3N2O6
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 480439-73-4
PubChem ID 52941382
InChI Key LPWONNPEPDHEAI-GJZGRUSLSA-N
Smiles OC([C@@H](N)[C@H](OCC1=CC=CC(NC(C2=CC=C(C(F)(F)F)C=C2)=O)=C1)C(O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 42.63 100

Preparing Stock Solutions

The following data is based on the product molecular weight 426.35. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.35 mL 11.73 mL 23.45 mL
5 mM 0.47 mL 2.35 mL 4.69 mL
10 mM 0.23 mL 1.17 mL 2.35 mL
50 mM 0.05 mL 0.23 mL 0.47 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Tsukada et al (2005) Effects of a novel glutamate transporter blocker, (2S, 3S)-3-{3-[4-(trifluoromethyl)benzoylamino]benzyloxy}aspartate (TFB-TBOA), on activities of hippocampal neurons. Neuropharmacology 48 479 PMID: 15755476

Shimamoto and Shigeri (2006) Elucidation of glutamate transporter functions using selective inhibitors. CNS Agents Med.Chem. 6 59 PMID:

Magi et al (2012) Physical and functional interaction of NCX1 and EAAC1 transporters leading to glutamate-enhanced ATP production in brain mitochondria. PLoS One 7 e34015 PMID: 22479505

Bozzo and Chatton (2010) Inhibitory effects of (2S, 3S)-3-[3-[4-(trifluoromethyl)benzoylamino]benzyloxy]aspartate (TFB-TBOA) on the astrocytic sodium responses to glutamate. Brain Res. 1316 PMID: 20026319


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Keywords: TFB-TBOA, supplier, High, affinity, EAAT1, EAAT2, blockers, EAAT, Excitatory, Amino, Acid, Transporters, GLAST, GLT-1, Glutamate, Monoamine, Neurotransmitter, Glutamate, Transporters, Tocris Bioscience

7 Citations for TFB-TBOA

Citations are publications that use Tocris products. Selected citations for TFB-TBOA include:

Zou et al (2011) Morphine potentiates neurodegenerative effects of HIV-1 Tat through actions at μ-opioid receptor-expressing glia. Brain 134 3616 PMID: 22102648

Canul-Tec (2017) Structure and allosteric inhibition of excitatory amino acid transporter 1. Nature PMID: 28424515

Obba et al (2015) The PRKAA1/AMPKα1 pathway triggers autophagy during CSF1-induced human monocyte differentiation and is a potential target in CMML. Mol Pain 11 1114 PMID: 26029847

Yan et al (2015) Paclitaxel induces acute pain via directly activating toll like receptor 4. Physiol Rep 11 10 PMID: 25868824

Fleischer et al (2015) High-frequency voltage oscillations in cultured astrocytes. PLoS One 3 PMID: 25969464

Ivanov et al (2014) Glycolysis and oxidative phosphorylation in neurons and astrocytes during network activity in hippocampal slices. J Cereb Blood Flow Metab 34 397 PMID: 24326389

Afzalov et al (2013) Low micromolar Ba(2+) potentiates glutamate transporter current in hippocampal astrocytes. Front Cell Neurosci 7 135 PMID: 24009556


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