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Cat.No. 6621 - TFAX 488, TFP | C27H14F4N2O11S2.C12H30N2 | CAS No. 878546-79-3
Description: Green fluorescent dye; amine reactive
Chemical Name: 3,6-Diamino-9-[2-carboxy-4-[(2,3,5,6-tetrafluorophenoxy)carbonyl]phenyl]-4,5-disulfoxanthylium bis(triethylammmonium) salt
Literature (1)

Biological Activity for TFAX 488, TFP

TFAX 488, TFP is an amine reactive green fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Less susceptible to spontaneous hydrolysis during conjugation reactions compared to SE or NHS dyes. Also displays greater stability compared to NHS and SE versions, typically lasting several hours. Suitable for use in flow cytometry, two-photon excitation microscopy (TPE), and super resolution microscopy techniques, such as dSTORM, SIM and STED. Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1cm-1; quantum yield = 0.92.

Optical Data for TFAX 488, TFP

TFAX 488, TFP Dye Spectra
Emission Color Green
λabs 495 nm
λem 515 nm
Extinction Coefficient (ε) 73,000 M-1cm-1
Quantum Yield (φ) 0.92
Closest Laser line 488 nm
Reactive Group TFP
Reactivity Primary amines
Cell Permeable No
pH Sensitivity pH insensitive pH 4-10
Application flow cytometry, two-photon excitation microscopy, dSTORM, SIM, STED
Alternative For Alexa Fluor® 488 TFP ester, AF 488, FITC, Cy2

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

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Technical Data for TFAX 488, TFP

M. Wt 884.91
Formula C27H14F4N2O11S2.C12H30N2
Storage Store at -20°C
CAS Number 878546-79-3
Smiles CCN(CC)CC.CCN(CC)CC.[NH2+]=C1C=CC2=C(C3=CC=C(C(OC4=C(F)C(F)=CC(F)=C4F)=O)C=C3C([O-])=O)C5=CC=C(N)C(S(=O)(O)=O)=C5OC2=C1S(=O)(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for TFAX 488, TFP

Solubility Soluble in DMSO

Product Datasheets for TFAX 488, TFP

References for TFAX 488, TFP

References are publications that support the biological activity of the product.

Schilling et al (2006) On the seeding and oligomerization of pGlu-amyloid peptides (in vitro). Biochemistry 45 12393 PMID: 17029395

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Keywords: TFAX 488, TFP, TFAX 488, TFP supplier, TFAX488, TFP, Amine, reactive, green, fluorescent, dye, dSTORM, two-photon, 2-photon, microscopy, flow, cytometry, TFAX, Dyes, Flow, Cytometry, 6621, Tocris Bioscience

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