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TFAX 488, TFP is an amine reactive green fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Less susceptible to spontaneous hydrolysis during conjugation reactions compared to SE or NHS dyes. Also displays greater stability compared to NHS and SE versions, typically lasting several hours. Suitable for use in flow cytometry, two-photon excitation microscopy (TPE), and super resolution microscopy techniques, such as dSTORM, SIM and STED. Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1cm-1; quantum yield = 0.92.
|Extinction Coefficient (ε)||73,000 M-1cm-1|
|Quantum Yield (φ)||0.92|
|Closest Laser line||488 nm|
|pH Sensitivity||pH insensitive pH 4-10|
|Application||flow cytometry, two-photon excitation microscopy, dSTORM, SIM, STED|
|Alternative For||Alexa Fluor® 488 TFP ester, AF 488, FITC, Cy2|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectral Viewer
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
References are publications that support the biological activity of the product.
Schilling et al (2006) On the seeding and oligomerization of pGlu-amyloid peptides (in vitro). Biochemistry 45 12393 PMID: 17029395
Keywords: TFAX 488, TFP, TFAX 488, TFP supplier, TFAX488, TFP, Amine, reactive, green, fluorescent, dye, dSTORM, two-photon, 2-photon, microscopy, flow, cytometry, TFAX, Dyes, Flow, Cytometry, 6621, Tocris Bioscience
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This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our: