Pricing Availability   Qty
Description: Superoxide scavenger; antioxidant
Chemical Name: 1-Oxyl-2,2,6,6-tetramethyl-4-hydroxypiperidine
Purity: ≥99%
Citations (9)
Reviews (1)

Biological Activity for Tempol

Tempol is a superoxide scavenger that displays neuroprotective, anti-inflammatory and analgesic effects. Tempol reduces γ-radiation-induced damage in hematopoietic stem cells in vitro and in vivo.

Technical Data for Tempol

M. Wt 172.24
Formula C9H18NO2
Storage Store at -20°C
Purity ≥99%
CAS Number 2226-96-2
PubChem ID 137994
Smiles CC1(C)CC(O)CC(C)(C)N1[O]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Tempol

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 17.22 100
ethanol 17.22 100

Preparing Stock Solutions for Tempol

The following data is based on the product molecular weight 172.24. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 5.81 mL 29.03 mL 58.06 mL
5 mM 1.16 mL 5.81 mL 11.61 mL
10 mM 0.58 mL 2.9 mL 5.81 mL
50 mM 0.12 mL 0.58 mL 1.16 mL

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Product Datasheets for Tempol

Certificate of Analysis / Product Datasheet
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References for Tempol

References are publications that support the biological activity of the product.

Francis and Sehgal (2006) The superoxide scavenger TEMPOL induces urok. receptor (uPAR) expression in human prostate cancer cells. Mol.Cancer 5 21 PMID: 16756681

Deng-Bryant et al (2008) Neuroprotective effect of tempol, a catalytic scavenger of peroxynitrite-derived free radicals, in a mouse traumatic brain injury model. J.Cereb.Blood Flow Metab. 28 1114 PMID: 18319733

Khattab (2006) TEMPOL, a membrane-permeable radical scavenger, attenuates peroxynitrite- and superoxide anion-enhanced carrageenan-induced paw edema and hyperalgesia: a key role for superoxide anion. EurJ.Pharmacol. 548 167 PMID: 16973155

Ramachandran et al (2012) Prevention of γ-radiation induced cellular genotoxicity by tempol: Protection of hematopoietic system. Environ.Toxicol.Pharmacol. 34 253 PMID: 22609778

If you know of a relevant reference for Tempol, please let us know.

Keywords: Tempol, Tempol supplier, Superoxide, scavenger, antioxidant, hematopoietic, haematopoietic, stem, cells, HSC, Antioxidants, Hematopoietic, Stem, Cells, 3082, Tocris Bioscience

9 Citations for Tempol

Citations are publications that use Tocris products. Selected citations for Tempol include:

Ruparel et al (2012) Plasticity of cytochrome P450 isozyme expression in rat trigeminal ganglia neurons during inflammation. J Biol Chem 153 2031 PMID: 22633978

Hood et al (2016) Nicotinamide Adenine Dinucleotide Phosphate Oxidase-Mediated Redox Signaling and Vascular Remodeling by 16α-Hydroxyestrone in Human Pulmonary Artery Cells: Implications in Pulmonary Arterial Hypertension. Hypertension 68 796 PMID: 27402919

Tomasovic et al (2015) Sestrin 2 Regulates PDGF Receptor β (PDGFRβ) Expression by Modulating Proteasomal and Nrf2 Transcription Factor Functions. Brain Res NULL PMID: 25716320

Nesuashvili et al (2013) Sensory nerve terminal mitochondrial dysfunction activates airway sensory nerves via transient receptor potential (TRP) channels. Mol Pharmacol 83 1007 PMID: 23444014

Do you know of a great paper that uses Tempol from Tocris? Please let us know.

Reviews for Tempol

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Tempol blunts 15d-PGJ2-induced ROS levels in HL-1 cardiomyocytes.
By Chintan Koyani on 10/13/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: HL-1 cardiomyocytes

HL-1 cardiomyocytes were incubated with Tempol (1 mM) for 30 min prior to 15d-PGJ2 treatments (15 μM) for 1 h to follow ROS generation using carboxy-H2DCFDA (10 μM). Figure shows that the antioxidant Tempol (a cell-permeable superoxide anion scavenger) blunted 15d-PGJ2-induced ROS formation efficiently.

PMID: 24698234
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