Spautin 1

Pricing Availability   Qty
Description: USP10 and USP13 inhibitor; inhibits autophagy
Chemical Name: 6-Fluoro-N-[(4-Fluorophenyl)methyl]-4-quinazolinamine
Purity: ≥98% (HPLC)
Citations (3)

Biological Activity for Spautin 1

Spautin 1 is an inhibitor of autophagy; inhibits USP10 and USP13 activity (IC50 values are 0.6 and 0.7 μM respectively) and promotes degradation of Vps34 (class III PI 3-kinase) complexes. Selectively promotes apoptosis of cancer cells under starvation conditions. Prevents the PDGF-induced conversion from the contractile to synthetic phenotype in vascular smooth muscle cells.

Compound Libraries for Spautin 1

Spautin 1 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for Spautin 1

M. Wt 271.26
Formula C15H11F2N3
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 1262888-28-7
PubChem ID 51037431
Smiles FC1=CC=C(N=CN=C2NCC3=CC=C(F)C=C3)C2=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Spautin 1

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 27.13 100

Preparing Stock Solutions for Spautin 1

The following data is based on the product molecular weight 271.26. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.69 mL 18.43 mL 36.87 mL
5 mM 0.74 mL 3.69 mL 7.37 mL
10 mM 0.37 mL 1.84 mL 3.69 mL
50 mM 0.07 mL 0.37 mL 0.74 mL

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References for Spautin 1

References are publications that support the biological activity of the product.

Salabei et al (2013) PDGF-mediated autophagy regulates vascular smooth muscle cell phenotype and resistance to oxidative stress. Biochem.J. 451 375 PMID: 23421427

Liu et al (2011) Beclin1 controls the levels of p53 by regulating the deubiquitination activity of USP10 and USP13. Cell 147 223 PMID: 21962518

If you know of a relevant reference for Spautin 1, please let us know.

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Keywords: Spautin 1, Spautin 1 supplier, class, III, PI3, kinase, autophagy, inhibitors, inhibits, USP10, USP13, deubiquitinating, enzymes, vps34, Deubiquitinating, Enzymes, Autophagy, 5197, Tocris Bioscience

3 Citations for Spautin 1

Citations are publications that use Tocris products. Selected citations for Spautin 1 include:

Catarina et al (2020) Antagonistic relationship between the unfolded protein response and myocardin-driven transcription in smooth muscle. J Cell Physiol 235 7370-7382 PMID: 32039481

Tinghu et al (2021) Targeting oncoproteins with a positive selection assay for protein degraders. Sci Adv 7 PMID: 33547076

Ji Eun et al (2022) USP13 regulates HMGB1 stability and secretion through its deubiquitinase activity. Mol Med 28 164 PMID: 36585612

Do you know of a great paper that uses Spautin 1 from Tocris? Please let us know.

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