SEA 0400

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Description: Potent Na+/Ca2+ exchanger (NCX) inhibitor
Chemical Name: 2-[4-[(2,5-Difluorophenyl)methoxy]phenoxy]-5-ethoxybenzenamine
Purity: ≥98% (HPLC)
Citations (3)

Biological Activity for SEA 0400

SEA 0400 is a potent Na+/Ca2+ exchanger (NCX) inhibitor. Inhibits NCX activity in rat astrocytes, microglia and cultured neurons, canine cardiac sarcolemmal vesicles and rat cardiomyocytes (IC50 values are 5 and 8.3, 33, 90 and 92 nM, respectively). Reduces infarct volumes and exhibits protective effects against myocardial ischemia in rats.

Compound Libraries for SEA 0400

SEA 0400 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for SEA 0400

M. Wt 371.38
Formula C21H19F2NO3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 223104-29-8
PubChem ID 644100
Smiles CCOC1=CC(N)=C(C=C1)OC2=CC=C(C=C2)OCC3=C(C=CC(F)=C3)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for SEA 0400

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 37.14 100
ethanol 37.14 100

Preparing Stock Solutions for SEA 0400

The following data is based on the product molecular weight 371.38. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.69 mL 13.46 mL 26.93 mL
5 mM 0.54 mL 2.69 mL 5.39 mL
10 mM 0.27 mL 1.35 mL 2.69 mL
50 mM 0.05 mL 0.27 mL 0.54 mL

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References for SEA 0400

References are publications that support the biological activity of the product.

Takahashi et al (2003) Protective effects of SEA0400, a novel and selective inhibitor of the Na+/Ca2+ exchanger, on myocardial ischemia-reperfusion injuries. Eur.J.Pharmacol. 458 155 PMID: 12498920

Christ et al (2016) Block of Na(+)/Ca(2+) exchanger by SEA0400 in human right atrial preparations from patients in sinus rhythm and in atrial fibrillation. Eur.J.Pharmacol. 788 286 PMID: 27373849

Karashima et al (2007) Involvement of Na+-Ca2+ exchanger in cAMP-mediated relaxation in mice aorta: evaluation using transgenic mice. Br.J.Pharmacol. 150 343 PMID: 17220909

If you know of a relevant reference for SEA 0400, please let us know.

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Keywords: SEA 0400, SEA 0400 supplier, SEA0400, Inhibitors, Inhibits, NCX, Na+/Ca2+, exchanger, cardioprotectant, Exchanger, 6164, Tocris Bioscience

3 Citations for SEA 0400

Citations are publications that use Tocris products. Selected citations for SEA 0400 include:

Neubert et al (2020) NCX1 Represents an Ionic Na+ Sensing Mechanism in Macrophages. PLoS Biol 18 PMID: 32569301

Yan et al (2019) Blockade of the forward Na+ /Ca2+ exchanger suppresses the growth of glioblastoma cells through Ca2+ -mediated cell death. Br J Pharmacol 176 2691-2707 PMID: 31034096

Bradley J et al (2019) Intracellular Ca2+ Homeostasis and Nuclear Export Mediate Exit from Naive Pluripotency. Cell Stem Cell 25 210-224.e6 PMID: 31104942

Do you know of a great paper that uses SEA 0400 from Tocris? Please let us know.

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