SEA 0400

Pricing Availability   Qty
Description: Potent Na+/Ca2+ exchanger (NCX) inhibitor
Chemical Name: 2-[4-[(2,5-Difluorophenyl)methoxy]phenoxy]-5-ethoxybenzenamine
Purity: ≥98% (HPLC)
Citations (1)
Literature (2)

Biological Activity for SEA 0400

SEA 0400 is a potent Na+/Ca2+ exchanger (NCX) inhibitor. Inhibits NCX activity in rat astrocytes, microglia and cultured neurons, canine cardiac sarcolemmal vesicles and rat cardiomyocytes (IC50 values are 5 and 8.3, 33, 90 and 92 nM, respectively). Reduces infarct volumes and exhibits protective effects against myocardial ischemia in rats.

Compound Libraries for SEA 0400

SEA 0400 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for SEA 0400

M. Wt 371.38
Formula C21H19F2NO3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 223104-29-8
PubChem ID 644100
Smiles CCOC1=CC(N)=C(C=C1)OC2=CC=C(C=C2)OCC3=C(C=CC(F)=C3)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for SEA 0400

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 37.14 100
ethanol 37.14 100

Preparing Stock Solutions for SEA 0400

The following data is based on the product molecular weight 371.38. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.69 mL 13.46 mL 26.93 mL
5 mM 0.54 mL 2.69 mL 5.39 mL
10 mM 0.27 mL 1.35 mL 2.69 mL
50 mM 0.05 mL 0.27 mL 0.54 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

References for SEA 0400

References are publications that support the biological activity of the product.

Takahashi et al (2003) Protective effects of SEA0400, a novel and selective inhibitor of the Na+/Ca2+ exchanger, on myocardial ischemia-reperfusion injuries. Eur.J.Pharmacol. 458 155 PMID: 12498920

Christ et al (2016) Block of Na(+)/Ca(2+) exchanger by SEA0400 in human right atrial preparations from patients in sinus rhythm and in atrial fibrillation. Eur.J.Pharmacol. 788 286 PMID: 27373849

Karashima et al (2007) Involvement of Na+-Ca2+ exchanger in cAMP-mediated relaxation in mice aorta: evaluation using transgenic mice. Br.J.Pharmacol. 150 343 PMID: 17220909

If you know of a relevant reference for SEA 0400, please let us know.

View Related Products by Product Action

View all Na+/Ca2+ Exchanger Inhibitors

Keywords: SEA 0400, SEA 0400 supplier, SEA0400, Inhibitors, Inhibits, NCX, Na+/Ca2+, exchanger, cardioprotectant, Exchanger, 6164, Tocris Bioscience

1 Citation for SEA 0400

Citations are publications that use Tocris products. Selected citations for SEA 0400 include:

Neubert et al (2020) NCX1 Represents an Ionic Na+ Sensing Mechanism in Macrophages. PLoS Biol 18 PMID: 32569301

Do you know of a great paper that uses SEA 0400 from Tocris? Please let us know.

Reviews for SEA 0400

There are currently no reviews for this product. Be the first to review SEA 0400 and earn rewards!

Have you used SEA 0400?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Cardiovascular Research Product Guide

Cardiovascular Research Product Guide

A collection of over 250 products for cardiovascular research, the guide includes research tools for the study of:

  • Hypertension
  • Thrombosis and Hemostasis
  • Atherosclerosis
  • Myocardial Infarction
  • Ischemia/Reperfusion Injury
  • Arrhythmias
  • Heart Failure
Epilepsy Poster

Epilepsy Poster

Epilepsy is a brain disease that affects 60 million people globally. More than 20 anti-seizure drugs are currently available, but these do not address the underlying causes of the condition. This poster summarizes current knowledge about the development of the condition and highlights some approaches that have disease-modifying effects in proof-of-concept studies.