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Biological Activity for Se-NADA
Se-NADA is an orange fluorescent benzoselenadiazole D-amino acid (FDAA) for imaging bacteria. It is covalently incorporated into peptidoglycans in the cell walls of live bacteria. It also acts as a photosensitizer, bringing about bacterial cell death on exposure to visible light. Se-NADA is effective against both Gram-positive and Gram-negative bacteria. Ex/Em = 470/590 nm.
Application of Se-NADA in B subtilis bacteria cells. Fluorescence microscopy image of B subtilis bacterial cells treated with SeNADA (250 μM, red) proving incorporation of the D-amino acid into the peptidoglycans of the cell wall. Image kindly provided by Dr Erkin Kuru, Department of Genetics, Harvard Medical School, Boston.
Sold under license from the University of Edinburgh
Optical Data for Se-NADA
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectral Viewer
Technical Data for Se-NADA
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Se-NADA
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Se-NADA
The following data is based on the product molecular weight 366.62. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.1 mM||27.28 mL||136.38 mL||272.76 mL|
|0.5 mM||5.46 mL||27.28 mL||54.55 mL|
|1 mM||2.73 mL||13.64 mL||27.28 mL|
|5 mM||0.55 mL||2.73 mL||5.46 mL|
References for Se-NADA
References are publications that support the biological activity of the product.
Benson et al (2021) Photoactivatable metabolic warheads enable precise and safe ablation of target cells in vivo. Nat Commun 12 2369 PMID: 33888691
If you know of a relevant reference for Se-NADA, please let us know.
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Keywords: Se-NADA, Se-NADA supplier, Fluorescence, imaging, Fluorescent, D-amino, acid, FDAA, labeling, labelling, peptidoglycans, bacteria, bacterial, cell, wall, benzoselenadiazole, photosensitizer, Probes, for, Imaging, Bacteria, Antibiotics, 7449, Tocris Bioscience
Citations for Se-NADA
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Literature in this Area
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
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