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Description: Orange fluorescent benzoselenadiazole D-amino acid (FDAA) for imaging bacteria; also photosensitizer
Chemical Name: 3-((7-Nitro-2,1,3-benzoselenadiazol-4-yl)amino)-D-alanine hydrochloride
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity for Se-NADA

Se-NADA is an orange fluorescent benzoselenadiazole D-amino acid (FDAA) for imaging bacteria. It is covalently incorporated into peptidoglycans in the cell walls of live bacteria. It also acts as a photosensitizer, bringing about bacterial cell death on exposure to visible light. Se-NADA is effective against both Gram-positive and Gram-negative bacteria. Ex/Em = 470/590 nm.

Scientific Data

Fluorescence Imaging Application of Se-NADA in B subtilis bacteria cells View Larger

Application of Se-NADA in B subtilis bacteria cells. Fluorescence microscopy image of B subtilis bacterial cells treated with SeNADA (250 μM, red) proving incorporation of the D-amino acid into the peptidoglycans of the cell wall. Image kindly provided by Dr Erkin Kuru, Department of Genetics, Harvard Medical School, Boston.

Licensing Information

Sold under license from the University of Edinburgh

Optical Data for Se-NADA

Se-NADA Dye Spectra
Emission Color Orange
λabs 470 nm
λem 590 nm
Cell Permeable Yes
Application Fluorescence Microscopy

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Technical Data for Se-NADA

M. Wt 366.62
Formula C9H9N5O4Se.HCl
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 2571574-70-2
Smiles N[C@@H](C(O)=O)CNC1=CC=C(N(=O)=O)C2=N[Se]N=C12.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Se-NADA

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 3.67 10

Preparing Stock Solutions for Se-NADA

The following data is based on the product molecular weight 366.62. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 27.28 mL 136.38 mL 272.76 mL
0.5 mM 5.46 mL 27.28 mL 54.55 mL
1 mM 2.73 mL 13.64 mL 27.28 mL
5 mM 0.55 mL 2.73 mL 5.46 mL

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References for Se-NADA

References are publications that support the biological activity of the product.

Benson et al (2021) Photoactivatable metabolic warheads enable precise and safe ablation of target cells in vivo. Nat Commun 12 2369 PMID: 33888691

If you know of a relevant reference for Se-NADA, please let us know.

Keywords: Se-NADA, Se-NADA supplier, Fluorescence, imaging, Fluorescent, D-amino, acid, FDAA, labeling, labelling, peptidoglycans, bacteria, bacterial, cell, wall, benzoselenadiazole, photosensitizer, Probes, for, Imaging, Bacteria, Antibiotics, 7449, Tocris Bioscience

Citations for Se-NADA

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Currently there are no citations for Se-NADA. Do you know of a great paper that uses Se-NADA from Tocris? Please let us know.

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Literature in this Area

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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