SAG 21k

Pricing Availability   Qty
Description: Hedgehog signaling activator; brain penetrant and orally bioavailable
Chemical Name: 3-Chloro-4,7-difluoro-N-[[2-methoxy-5-(4-pyridinyl)phenyl]methyl]-N-trans-4-(methylamino)cyclohexyl]benzo[b]thiophene-2-carboxamide
Purity: ≥98% (HPLC)
Citations (3)
Literature (4)

Biological Activity for SAG 21k

SAG 21k is a potent Hedgehog signaling activator (EC50 = 0.4 nM). Orally bioavailable and brain penetrant.

Compound Libraries for SAG 21k

SAG 21k is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for SAG 21k

M. Wt 556.07
Formula C29H28ClF2N3O2S
Storage Desiccate at -20°C
Purity ≥98% (HPLC)
CAS Number 946002-48-8
PubChem ID 16678532
Smiles COC1=C(CN([C@@H]2CC[C@@H](NC)CC2)C(C3=C(Cl)C4=C(C(F)=CC=C4F)S3)=O)C=C(C5=CC=NC=C5)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for SAG 21k

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 55.61 100
ethanol 11.12 20

Preparing Stock Solutions for SAG 21k

The following data is based on the product molecular weight 556.07. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.8 mL 8.99 mL 17.98 mL
5 mM 0.36 mL 1.8 mL 3.6 mL
10 mM 0.18 mL 0.9 mL 1.8 mL
50 mM 0.04 mL 0.18 mL 0.36 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for SAG 21k

Certificate of Analysis / Product Datasheet
Select another batch:

References for SAG 21k

References are publications that support the biological activity of the product.

Brunton et al (2009) Potent agonists of the Hedgehog signaling pathway. Bioorg.Med.Chem.Lett. 19 4308 PMID: 19500978

If you know of a relevant reference for SAG 21k, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Hedgehog Signaling Activators

Keywords: SAG 21k, SAG 21k supplier, SAG21k, Hedgehog, Hh, signaling, activators, activation, orally, bioavailable, brain, penetrant, potent, Signaling, 5282, Tocris Bioscience

3 Citations for SAG 21k

Citations are publications that use Tocris products. Selected citations for SAG 21k include:

M et al (2021) Hedgehog signaling promotes endoneurial fibroblast migration and Vegf-A expression following facial nerve injury. Brain Res 1751 147204 PMID: 33189691

Olivier et al (2022) Paraxial mesoderm organoids model development of human somites. Elife 11 PMID: 35088712

Norris et al (2023) Hedgehog signaling via its ligand DHH acts as cell fate determinant during skeletal muscle regeneration Nat Commun 14 3766 PMID: 37355632

Do you know of a great paper that uses SAG 21k from Tocris? Please let us know.

Reviews for SAG 21k

There are currently no reviews for this product. Be the first to review SAG 21k and earn rewards!

Have you used SAG 21k?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Stem Cell Research Product Guide

Stem Cell Research Product Guide

This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:

  • Self-renewal and Maintenance
  • Differentiation
  • Reprogramming
  • Organoid Generation
  • GMP and Ancillary Material Grade Products
Stem Cells Scientific Review

Stem Cells Scientific Review

Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.

Stem Cell Workflow Poster

Stem Cell Workflow Poster

Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.

Stem Cells Poster

Stem Cells Poster

Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.