SAG 21k

Pricing Availability Delivery Time Qty
Cat.No. 5282 - SAG 21k | C29H28ClF2N3O2S | CAS No. 946002-48-8
Description: Hedgehog signaling activator; brain penetrant and orally bioavailable
Chemical Name: 3-Chloro-4,7-difluoro-N-[[2-methoxy-5-(4-pyridinyl)phenyl]methyl]-N-trans-4-(methylamino)cyclohexyl]benzo[b]thiophene-2-carboxamide
Purity: ≥98% (HPLC)

Biological Activity

Potent Hedgehog signaling activator (EC50 = 0.4 nM). Orally bioavailable and brain penetrant.

Technical Data

M. Wt 556.07
Formula C29H28ClF2N3O2S
Storage Desiccate at -20°C
Purity ≥98% (HPLC)
CAS Number 946002-48-8
PubChem ID 16678532
Smiles COC1=C(CN([C@@H]2CC[C@@H](NC)CC2)C(C3=C(Cl)C4=C(C(F)=CC=C4F)S3)=O)C=C(C5=CC=NC=C5)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 55.61 100
ethanol 11.12 20

Preparing Stock Solutions

The following data is based on the product molecular weight 556.07. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.8 mL 8.99 mL 17.98 mL
5 mM 0.36 mL 1.8 mL 3.6 mL
10 mM 0.18 mL 0.9 mL 1.8 mL
50 mM 0.04 mL 0.18 mL 0.36 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets

Safety Datasheet


References are publications that support the products' biological activity.

Brunton et al (2009) Potent agonists of the Hedgehog signaling pathway. Bioorg.Med.Chem.Lett. 19 4308 PMID: 19500978

If you know of a relevant reference for SAG 21k, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Hedgehog Signaling Activators

Keywords: SAG 21k, supplier, SAG21k, Hedgehog, Hh, signaling, activators, activation, orally, bioavailable, brain, penetrant, potent, Hedgehog, Signaling, Tocris Bioscience

Citations for SAG 21k

Citations are publications that use Tocris products.

Currently there are no citations for SAG 21k. Do you know of a great paper that uses SAG 21k from Tocris? If so please let us know.

Commented out for usability testing


TODO: Add Reviews

Literature in this Area


Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Stem Cells

Stem Cells Scientific Review

Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.

Pathways for SAG 21k


TODO: Add Protocols