Ruthenium-bipyridine-trimethylphosphine caged glutamate that can be excited by visible wavelengths and releases glutamate after one- or two-photon excitation. Has high quantum efficiency and can be used at low concentrations, partly avoiding blockade of GABAergic transmission seen with other caged glutamate compounds. Displays high spatial resolution and generates excitatory responses in individual dendritic spines with physiological kinetics.
Sold under licence from Columbia University
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 970.54. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.03 mL||5.15 mL||10.3 mL|
|5 mM||0.21 mL||1.03 mL||2.06 mL|
|10 mM||0.1 mL||0.52 mL||1.03 mL|
|50 mM||0.02 mL||0.1 mL||0.21 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Fino et al (2009) RuBi-Glutamate: two-photon and visible-light photoactivation of neurons and dendritic spines. Front.Neural Circuits 3 1 PMID: 19506708
Salierno et al (2010) A fast ruthenium polypyridine cage complex photoreleases glutamate with visible or IR light in one and two photon regimes. J.Inorg.Biochem. 104 418 PMID: 20060592
If you know of a relevant reference for RuBi-Glutamate, please let us know.
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Keywords: Caged Glutamate mGluR iGluR Receptors agonists ionotropic metabotropic Caged Glutamate Compounds
1 Citation for RuBi-Glutamate
Citations are publications that use Tocris products. Selected citations for RuBi-Glutamate include:
Blaesse et al (2015) μ-Opioid Receptor-Mediated Inhibition of Intercalated Neurons and Effect on Synaptic Transmission to the Central Amygdala. Neuroscience 35 7317 PMID: 25972162
Do you know of a great paper that uses RuBi-Glutamate from Tocris? If so please let us know.
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