Pricing Availability   Qty
Description: Selective P2X3 and P2X2/3 antagonist
Chemical Name: 5-[[4,5-Dimethoxy-2-(methylethyl)phenyl]methyl]-2,4-pyrimidinediamine
Purity: ≥98% (HPLC)
Literature (1)

Biological Activity for RO-3

RO-3 is a selective homomeric P2X3 and heteromeric P2X2/3 receptor antagonist (pIC50 values are 7.0 and 5.9 respectively) that exhibits no activity at P2X1, P2X2, P2X4, P2X5 and P2X7 receptors (IC50 > 10 μM). Attenuates nociceptive sensitivity in animal models of pain. Orally active and brain penetrant.

Technical Data for RO-3

M. Wt 302.37
Formula C16H22N4O2
Storage Store at RT
Purity ≥98% (HPLC)
CAS Number 1026582-88-6
PubChem ID 11289644
Smiles NC1=NC(N)=NC=C1CC2=C(C(C)C)C=C(OC)C(OC)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for RO-3

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 30.24 100

Preparing Stock Solutions for RO-3

The following data is based on the product molecular weight 302.37. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.31 mL 16.54 mL 33.07 mL
5 mM 0.66 mL 3.31 mL 6.61 mL
10 mM 0.33 mL 1.65 mL 3.31 mL
50 mM 0.07 mL 0.33 mL 0.66 mL

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Product Datasheets for RO-3

Certificate of Analysis / Product Datasheet
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References for RO-3

References are publications that support the biological activity of the product.

Ford et al (2006) Purinoceptors as therapeutic targets for lower urinary tract dysfunction. Br.J.Pharmacol. 147 S132 PMID: 16465177

Donnelly-Roberts et al (2008) Painful purinergic receptors. J.Pharmacol.Exp.Ther. 324 409 PMID: 18042830

Gever et al (2006) Pharmacology of P2X channels. Eur.J.Physiol. 452 513

If you know of a relevant reference for RO-3, please let us know.

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Keywords: RO-3, RO-3 supplier, Selective, P2X2, P2X2/3, antagonists, Receptors, Purinergic, purinoceptors, P2X, 3052, Tocris Bioscience

Citations for RO-3

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Currently there are no citations for RO-3. Do you know of a great paper that uses RO-3 from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

P2X and P2Y Receptors Scientific Review

P2X and P2Y Receptors Scientific Review

Written by Kenneth Jacobson, this review provides an overview of the different subtypes and structures of the P2 receptor families, as well as the pharmacological probes used to study them; compounds available from Tocris are listed.