Pricing Availability   Qty
Description: Selective TRPC3 inhibitor
Chemical Name: 1-[4-[(2,3,3-Trichloro-1-oxo-2-propen-1-yl)amino]phenyl]-5-(trifluoromethyl)-1H-pyrazole-4-carboxylic acid
Purity: ≥98% (HPLC)
Citations (5)
Reviews (1)

Biological Activity for Pyr3

Pyr3 is a selective antagonist of the canonical transient receptor potential channel 3 (TRPC3). Inhibits TRPC3-mediated Ca2+ influx (IC50 = 0.7 μM) and suppresses activation of nuclear factor of activated T cells (NFAT). Inhibits hypertrophic responses in cardiomyocytes.

Technical Data for Pyr3

M. Wt 456.63
Formula C16H11Cl3F3N3O3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1160514-60-2
PubChem ID 56964346
Smiles O=C(/C(Cl)=C(Cl)\Cl)NC(C=C2)=CC=C2N1N=CC(C(OCC)=O)=C1C(F)(F)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Pyr3

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 45.66 100

Preparing Stock Solutions for Pyr3

The following data is based on the product molecular weight 456.63. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.19 mL 10.95 mL 21.9 mL
5 mM 0.44 mL 2.19 mL 4.38 mL
10 mM 0.22 mL 1.09 mL 2.19 mL
50 mM 0.04 mL 0.22 mL 0.44 mL

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Product Datasheets for Pyr3

Certificate of Analysis / Product Datasheet
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References for Pyr3

References are publications that support the biological activity of the product.

Kiyonaka et al (2009) Selective and direct inhibition of TRPC3 channels underlies biological activities of a pyrazole compound. Proc.Natl.Acad.Sci. 106 5400

If you know of a relevant reference for Pyr3, please let us know.

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Keywords: Pyr3, Pyr3 supplier, canonical, transient, receptor, potential, TRPC3, channels, inhibitors, inhibits, TRPC, 3751, Tocris Bioscience

5 Citations for Pyr3

Citations are publications that use Tocris products. Selected citations for Pyr3 include:

Senadheera et al (2013) Enhanced contractility in pregnancy is associated with augmented TRPC3, L-type, and T-type voltage-dependent calcium channel function in rat uterine radial artery. ACS Chem Neurosci 305 R917 PMID: 23948776

Lee et al (2013) TRPM2 channels are required for NMDA-induced burst firing and contribute to H(2)O(2)-dependent modulation in substantia nigra pars reticulata GABAergic neurons. J Neurosci 33 1157 PMID: 23325252

Zhou et al (2014) Cerebellar modules operate at different frequencies. Elife 3 e02536 PMID: 24843004

Y S et al (2020) Hydrogen sulfide, oxygen, and calcium regulation in developing human airway smooth muscle. FASEB J 34 12991-13004 PMID: 32777143

Szteyn et al (2015) Divergence in endothelin-1- and bradykinin-activated store-operated calcium entry in afferent sensory neurons. Am J Physiol Regul Integr Comp Physiol 7 PMID: 25873305

Do you know of a great paper that uses Pyr3 from Tocris? Please let us know.

Reviews for Pyr3

Average Rating: 5 (Based on 1 Review.)

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Pyr3, a selective TRPC3 channel blocker in mesangial cell.
By Hitesh Soni on 01/04/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Mesangial Cell

For in vitro study to block the TRP ion channel and elucidate the mechanism of proliferation of mesangial cells under normal and high glucose conditions and to understand the role of TRP ion channels in diabetic nephropathy.

PMID: 29273760 Reference
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