Purvalanol B

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Cat.No. 1581 - Purvalanol B | C20H25ClN6O3 | CAS No. 212844-54-7
Description: Cyclin-dependent kinase inhibitor
Alternative Names: NG 95
Chemical Name: (2R)-2-[[6-[(3-Chloro-4-carboxyphenyl)amino]-9-(1-methylethyl)-9H-purin-2-yl]amino]-3-methyl-1-butanol
Purity: ≥99% (HPLC)
Datasheet
Citations (2)
Reviews
Literature

Biological Activity

Cyclin-dependent kinase inhibitor. IC50 values are 6, 6, 9, > 10,000, and 6 nM for cdc2/cyclin B, cdk2/cyclin A, cdk2/cyclin E, cdk4/cyclin D1 and cdk5-p35 respectively. Selective over a range of other protein kinases (IC50 > 10,000 nM). Shown to have antiproliferative properties, mediated by p42/p44 MAPK.

Licensing Information

Sold under license from the Regents of the University of California

Compound Libraries

Purvalanol B is also offered as part of the Tocriscreen Plus and Tocriscreen Kinase Inhibitor Toolbox I. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 432.91
Formula C20H25ClN6O3
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 212844-54-7
PubChem ID 448991
InChI Key ZKDXRFMOHZVXSG-HNNXBMFYSA-N
Smiles CC(C)[C@H](CO)NC1=NC(NC2=CC(Cl)=C(C=C2)C(O)=O)=C2N=CN(C(C)C)C2=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
1eq. NaOH 43.29 100 with gentle warming
DMSO 43.29 100

Preparing Stock Solutions

The following data is based on the product molecular weight 432.91. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.31 mL 11.55 mL 23.1 mL
5 mM 0.46 mL 2.31 mL 4.62 mL
10 mM 0.23 mL 1.15 mL 2.31 mL
50 mM 0.05 mL 0.23 mL 0.46 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References

References are publications that support the biological activity of the product.

Gray et al (1998) Exploiting chemical libraries, structure, and genomics in the search for kinase inhibitors. Science 281 533 PMID: 9677190

Gray et al (1999) ATP-site directed inhibitors of cyclin-dependent kinases. Curr.Med.Chem. 6 859 PMID: 10495356

Knockaert et al (2002) p42/p44 MAPKs are intracellular targets of the CDK inhibitor purvalanol. Oncogene 21 6413 PMID: 12226745


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Keywords: Purvalanol B, Purvalanol B supplier, Cyclin-dependent, protein, kinases, inhibitors, inhibits, Cdk, PurvalanolB, NG95, NG, 95, Kinase, 1581, Tocris Bioscience

2 Citations for Purvalanol B

Citations are publications that use Tocris products. Selected citations for Purvalanol B include:

Wissing et al (2007) Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry. J Pharmacol Exp Ther 6 537 PMID: 17192257

Schreiber et al (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9 1047 PMID: 20071362


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