PT 1

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Cat.No. 4039 - PT 1 | C23H16ClN3O6S | CAS No. 331002-70-1
Description: AMPK activator
Chemical Name: 2-Chloro-5-[[5-[[5-(4,5-Dimethyl-2-nitrophenyl)-2-furanyl]methylene]-4,5-dihydro-4-oxo-2-thiazolyl]amino]benzoic acid
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature
Pathways

Biological Activity

AMP-activated protein kinase (AMPK) activator. Stimulates AMPK heterotrimer (α1β1γ1) activity (EC50 = 0.3 μM). Selectively increases the activity of γ1- but not γ3-containing complexes. Thought to directly activate AMPK by antagonizing autoinhibition.

Technical Data

M. Wt 497.91
Formula C23H16ClN3O6S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 331002-70-1
PubChem ID 5753734
InChI Key RTHRCOIONCZINZ-JMIUGGIZSA-N
Smiles ClC1=C(C(O)=O)C=C(/N=C3/NC(/C(S3)=C/C2=CC=C(C4=C([N+]([O-])=O)C=C(C)C(C)=C4)O2)=O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
1.1eq. NaOH 49.79 100
DMSO 49.79 100

Preparing Stock Solutions

The following data is based on the product molecular weight 497.91. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.01 mL 10.04 mL 20.08 mL
5 mM 0.4 mL 2.01 mL 4.02 mL
10 mM 0.2 mL 1 mL 2.01 mL
50 mM 0.04 mL 0.2 mL 0.4 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Zorn and Wells (2010) Turning enzymes ON with small molecules. Nat.Chem.Biol. 6 179 PMID: 20154666

Pang et al (2007) Small molecule antagonizes autoinhibition and activates AMP-activated protein kinase in cells. J.Biol.Chem. 283 16051 PMID:

Jensen et al (2015) PT-1 selectively activates AMPK-γ1 complexes in mouse skeletal muscle, but activates all three γ subunit complexes in cultured human cells by inhibiting the respiratory chain. Biochem.J. 467 461 PMID: 25695398


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Keywords: AMPK amp-activated protein kinase activators PT1 AMPK

Citations for PT 1

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Literature in this Area

Kinases

Kinases Product Listing

A collection of over 400 products for kinase research, the listing includes inhibitors of:

  • Receptor Tyrosine Kinases
  • Protein Kinases A, C, D and G
  • PI-3 Kinase, Akt and mTOR
  • MAPK Signaling
  • Receptor Serine/Threonine Kinases

Pathways for PT 1

AMPK

AMPK Signaling Pathway

AMPK signaling pathway plays an important role in the cellular reponse to low levels of available ATP, often caused by stresses such as heat shock, ischemia or hypoxia.

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