PKC β pseudosubstrate

Pricing Availability Delivery Time Qty
Cat.No. 1792 - PKC b pseudosubstrate | H-Cys-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-OH H-Cys-Arg-Phe-Ala-Arg-Lys-Gly-Ala-Leu-Arg-Gln-Lys-Asn-Val-OH (Disulfide bridge: 17 -1*) | CAS No. 172308-76-8
Description: Selective cell-permeable PKC inhibitor peptide (attached to vector)
Alternative Names: Protein kinase C beta pseudosubstrate
Datasheet
Citations
Literature

Biological Activity

Selective cell-permeable peptide inhibitor of protein kinase C (IC50 ~ 0.5 μM). Consists of amino acids 19 - 31 of PKC pseudosubstrate domain linked by a disulphide bridge to a cell permeabilisation Antennapedia domain vector peptide. The Antennapedia peptide is actively taken up by intact cells, at 4 or 37°C, ensuring rapid and effective uptake of the inhibitor peptide. Once inside the cell, the disulphide bonds are subjected to reduction in the cytoplasm leading to release of the inhibitor peptide.

Technical Data

M. Wt 3994.84
Formula C177H294N62O38S3
Sequence CRQIKIWFQNRRMKWKK CRFARKGALRQKNV*

(Modifications: Disulfide bridge between 17 -1*)

Storage Store at -20°C
CAS Number 172308-76-8
PubChem ID 90488712
InChI Key KIWJPYSSLFMLBZ-OSCDWKIESA-N
Smiles [H]N[C@@H](CSSC[C@H](N[H])C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C(C)C)C(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

SolubilitySoluble to 1 mg/ml in water

Preparing Stock Solutions

The following data is based on the product molecular weight 3994.84. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 0.25 mL 1.25 mL 2.5 mL
5 mM 0.05 mL 0.25 mL 0.5 mL
10 mM 0.03 mL 0.13 mL 0.25 mL
50 mM 0.01 mL 0.03 mL 0.05 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Certificate of Analysis is currently unavailable
on-line. Please contact Customer Service.
Safety Datasheet

References

References are publications that support the products' biological activity.

Derossi et al (1994) The third helix of the antennapedia homeodomain translocates through biological membranes. J.Biol.Chem. 269 10444 PMID: 8144628

House and Kemp (1987) Protein kinase C contains a pseudosubstrate prototope in its regulatory domain. Science 238 1726 PMID: 3686012

Theodore et al (1995) Intraneuronal delivery of protein kinase C pseudosubstrate leads to growth cone collapse. J.Neurosci. 15 7158 PMID: 7472470


If you know of a relevant reference for PKC β pseudosubstrate, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Protein Kinase C Inhibitors

Keywords: PKC b pseudosubstrate, supplier, Selective, cell-permeable, PKC, inhibitors, inhibits, Protein, Kinases, C, beta, pseudosubstrate, PKCb, Protein, kinase, C, beta, pseudosubstrate, Protein, Kinase, C, Tocris Bioscience

Citations for PKC β pseudosubstrate

Citations are publications that use Tocris products.

Currently there are no citations for PKC β pseudosubstrate. Do you know of a great paper that uses PKC β pseudosubstrate from Tocris? If so please let us know.

Commented out for usability testing

Reviews

TODO: Add Reviews

Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Kinases

Kinases Product Listing

A collection of over 400 products for kinase research, the listing includes inhibitors of:

  • Receptor Tyrosine Kinases
  • Protein Kinases A, C, D and G
  • PI-3 Kinase, Akt and mTOR
  • MAPK Signaling
  • Receptor Serine/Threonine Kinases
Pain

Pain Research Product Guide

A collection of over 250 products for pain research, the guide includes research tools for the study of:

  • Nociception
  • Ion Channels
  • G-Protein-Coupled Receptors
  • Intracellular Signaling

Pathways for PKC β pseudosubstrate

Protocols

TODO: Add Protocols