Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewPhorbol 12,13-dibutyrate
Description: Protein kinase C activator
Alternative Names: PDBu
Chemical Name: (1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-1a,1b,4,4a,5,7a,7b,8,9,9a-Decahydro-4a,7b-dihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1H-cyclopropa[3,4]benz[1,2-e]azulen-9,9a-diyl butanoic acid ester
Purity: ≥98% (HPLC)
Biological Activity
Protein kinase C activator. Induces contraction of vascular smooth muscle and inhibits MLC phosphatase (MLCP) in vascular smooth muscle. Activity does not alter intracellular Ca2+ concentration. Also inhibits the activity of Na+,K+ ATPase in OK cells.
Technical Data
| M. Wt | 504.61 |
| Formula | C28H40O8 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 37558-16-0 |
| PubChem ID | 37783 |
| InChI Key | BQJRUJTZSGYBEZ-YVQNUNKESA-N |
| Smiles | OCC1=C[C@]([C@@](C(C)4C)([H])[C@@]4(OC(CCC)=O)[C@H](OC(CCC)=O)[C@H]3C)([H])[C@@]3(O)[C@@](C=C(C)C2=O)([H])[C@]2(O)C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 25.23 | 50 |
Preparing Stock Solutions
The following data is based on the product molecular weight 504.61. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 3.96 mL | 19.82 mL | 39.63 mL |
| 2.5 mM | 0.79 mL | 3.96 mL | 7.93 mL |
| 5 mM | 0.4 mL | 1.98 mL | 3.96 mL |
| 25 mM | 0.08 mL | 0.4 mL | 0.79 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets
References
References are publications that support the biological activity of the product.
Middleton et al (1993) Heterogeneity of protein kinase C-mediated rapid regulation of Na/K-ATPase in kidney epithelial cells. J.Biol.Chem. 268 15958 PMID: 8393456
Hori et al (1999) Presynaptic mechanism for phorphol ester-induced synaptic potentiation. J.Neurosci. 19 7262 PMID: 10460232
Sakai et al (2010) Augmented PDBu-mediated contaction of bronchial smooth muscle of mice with antigen-induced airway hyperresponsiveness. J.Smooth Muscle Res. 46 259 PMID: 21187674
If you know of a relevant reference for Phorbol 12,13-dibutyrate, please let us know.
View Related Products by Target
View Related Products by Product Action
Keywords: Phorbol 12,13-dibutyrate, Phorbol 12,13-dibutyrate supplier, PDBu, protein, kinases, c, pkc, activators, phorbol, esters, Protein, Kinase, C, Enzyme, Substrates, /, Activators, 4153, Tocris Bioscience
3 Citations for Phorbol 12,13-dibutyrate
Citations are publications that use Tocris products. Selected citations for Phorbol 12,13-dibutyrate include:
Sánchez et al (2014) Endothelin-1 contributes to endothelial dysfunction and enhanced vasoconstriction through augmented superoxide production in penile arteries from Ins-resistant obese rats: role of ET(A) and ET(B) receptors. Br J Pharmacol 171 5682 PMID: 25091502
Nostro et al (2015) Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines. J Cell Biol 4 591 PMID: 25843049
Nieuwenhuijze et al (2018) Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations. Front Immunol 9 2234 PMID: 30323813
Do you know of a great paper that uses Phorbol 12,13-dibutyrate from Tocris? Please let us know.
Reviews for Phorbol 12,13-dibutyrate
Average Rating: 5 (Based on 1 Review.)
Have you used Phorbol 12,13-dibutyrate?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
PDB reduces saxagliptin-induced APD prolongation in cardiomyocytes.
By
Chintan Koyani on 10/22/2018
Assay Type: In Vitro
Species: Other
Cell Line/Tissue: ventricular cardiomyocytes
Guinea pig ventricular cardiomyocytes were patched and superfused with Saxagliptin (2 µM, 5 min) followed by Phorbol 12,13-dibutyrate (PDB, 10 nM, 5 min) and action potentials were stimulated at a 1 Hz frequency. Action potential duration (APD) were analysed at 20%, 50% and 90% repolarization (shown in Image). PDB reduced saxagliptin-induced APD prolongation.
Protocols for Phorbol 12,13-dibutyrate
The following protocol features additional information for the use of Phorbol 12,13-dibutyrate (Cat. No. 4153).
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Cancer Research Product Guide
A collection of over 750 products for cancer research, the guide includes research tools for the study of:
- Cancer Metabolism
- Epigenetics in Cancer
- Receptor Signaling
- Cell Cycle and DNA Damage Repair
- Angiogenesis
- Invasion and Metastasis
Kinases Product Listing
A collection of over 400 products for kinase research, the listing includes inhibitors of:
- Receptor Tyrosine Kinases
- Protein Kinases A, C, D and G
- PI-3 Kinase, Akt and mTOR
- MAPK Signaling
- Receptor Serine/Threonine Kinases
Pain Research Product GuideUpdated
A collection of over 280 products for pain research, the guide includes research tools for the study of:
- Nociception
- Ion Channels
- G-Protein-Coupled Receptors
- Intracellular Signaling