Phorbol 12,13-dibutyrate

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Description: Protein kinase C activator
Alternative Names: PDBu
Chemical Name: (1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-1a,1b,4,4a,5,7a,7b,8,9,9a-Decahydro-4a,7b-dihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1H-cyclopropa[3,4]benz[1,2-e]azulen-9,9a-diyl butanoic acid ester
Purity: ≥97% (HPLC)
Citations (3)
Reviews (1)
Protocols (1)
Literature (3)

Biological Activity for Phorbol 12,13-dibutyrate

Phorbol 12,13-dibutyrate is a protein kinase C activator. Induces contraction of vascular smooth muscle and inhibits MLC phosphatase (MLCP) in vascular smooth muscle. Activity does not alter intracellular Ca2+ concentration. Also inhibits the activity of Na+,K+ ATPase in OK cells.

For more information about how Phorbol 12,13-dibutyrate may be used, see our protocol: Generation of β cells from hPSCs

Technical Data for Phorbol 12,13-dibutyrate

M. Wt 504.61
Formula C28H40O8
Storage Store at -20°C
Purity ≥97% (HPLC)
CAS Number 37558-16-0
PubChem ID 37783
Smiles OCC1=C[C@]([C@@](C(C)4C)([H])[C@@]4(OC(CCC)=O)[C@H](OC(CCC)=O)[C@H]3C)([H])[C@@]3(O)[C@@](C=C(C)C2=O)([H])[C@]2(O)C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Phorbol 12,13-dibutyrate

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 25.23 50

Preparing Stock Solutions for Phorbol 12,13-dibutyrate

The following data is based on the product molecular weight 504.61. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 3.96 mL 19.82 mL 39.63 mL
2.5 mM 0.79 mL 3.96 mL 7.93 mL
5 mM 0.4 mL 1.98 mL 3.96 mL
25 mM 0.08 mL 0.4 mL 0.79 mL

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Product Datasheets for Phorbol 12,13-dibutyrate

Certificate of Analysis / Product Datasheet
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References for Phorbol 12,13-dibutyrate

References are publications that support the biological activity of the product.

Middleton et al (1993) Heterogeneity of protein kinase C-mediated rapid regulation of Na/K-ATPase in kidney epithelial cells. J.Biol.Chem. 268 15958 PMID: 8393456

Hori et al (1999) Presynaptic mechanism for phorphol ester-induced synaptic potentiation. J.Neurosci. 19 7262 PMID: 10460232

Sakai et al (2010) Augmented PDBu-mediated contaction of bronchial smooth muscle of mice with antigen-induced airway hyperresponsiveness. J.Smooth Muscle Res. 46 259 PMID: 21187674

If you know of a relevant reference for Phorbol 12,13-dibutyrate, please let us know.

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Keywords: Phorbol 12,13-dibutyrate, Phorbol 12,13-dibutyrate supplier, PDBu, protein, kinases, c, pkc, activators, phorbol, esters, Protein, Kinase, C, Enzyme, Substrates, /, Activators, 4153, Tocris Bioscience

3 Citations for Phorbol 12,13-dibutyrate

Citations are publications that use Tocris products. Selected citations for Phorbol 12,13-dibutyrate include:

Sánchez et al (2014) Endothelin-1 contributes to endothelial dysfunction and enhanced vasoconstriction through augmented superoxide production in penile arteries from Ins-resistant obese rats: role of ET(A) and ET(B) receptors. Br J Pharmacol 171 5682 PMID: 25091502

Nostro et al (2015) Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines. J Cell Biol 4 591 PMID: 25843049

Nieuwenhuijze et al (2018) Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations. Front Immunol 9 2234 PMID: 30323813

Do you know of a great paper that uses Phorbol 12,13-dibutyrate from Tocris? Please let us know.

Reviews for Phorbol 12,13-dibutyrate

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PDB reduces saxagliptin-induced APD prolongation in cardiomyocytes.
By Chintan Koyani on 10/22/2018
Assay Type: In Vitro
Species: Other
Cell Line/Tissue: ventricular cardiomyocytes

Guinea pig ventricular cardiomyocytes were patched and superfused with Saxagliptin (2 µM, 5 min) followed by Phorbol 12,13-dibutyrate (PDB, 10 nM, 5 min) and action potentials were stimulated at a 1 Hz frequency. Action potential duration (APD) were analysed at 20%, 50% and 90% repolarization (shown in Image). PDB reduced saxagliptin-induced APD prolongation.

review image

Protocols for Phorbol 12,13-dibutyrate

The following protocol features additional information for the use of Phorbol 12,13-dibutyrate (Cat. No. 4153).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

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