Phorbol 12,13-dibutyrate

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Cat.No. 4153 - Phorbol 12,13-dibutyrate | C28H40O8 | CAS No. 37558-16-0
Description: Protein kinase C activator
Alternative Names: PDBu
Chemical Name: (1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-1a,1b,4,4a,5,7a,7b,8,9,9a-Decahydro-4a,7b-dihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1H-cyclopropa[3,4]benz[1,2-e]azulen-9,9a-diyl butanoic acid ester
Purity: ≥98% (HPLC)
Datasheet
Citations (1)
Literature

Biological Activity

Protein kinase C activator. Induces contraction of vascular smooth muscle and inhibits MLC phosphatase (MLCP) in vascular smooth muscle. Activity does not alter intracellular Ca2+ concentration. Also inhibits the activity of Na+,K+ ATPase in OK cells.

Technical Data

M. Wt 504.61
Formula C28H40O8
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 37558-16-0
PubChem ID 37783
InChI Key BQJRUJTZSGYBEZ-YVQNUNKESA-N
Smiles OCC1=C[C@]([C@@](C(C)4C)([H])[C@@]4(OC(CCC)=O)[C@H](OC(CCC)=O)[C@H]3C)([H])[C@@]3(O)[C@@](C=C(C)C2=O)([H])[C@]2(O)C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 25.23 50

Preparing Stock Solutions

The following data is based on the product molecular weight 504.61. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.98 mL 9.91 mL 19.82 mL
5 mM 0.4 mL 1.98 mL 3.96 mL
10 mM 0.2 mL 0.99 mL 1.98 mL
50 mM 0.04 mL 0.2 mL 0.4 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Middleton et al (1993) Heterogeneity of protein kinase C-mediated rapid regulation of Na/K-ATPase in kidney epithelial cells. J.Biol.Chem. 268 15958 PMID: 8393456

Hori et al (1999) Presynaptic mechanism for phorphol ester-induced synaptic potentiation. J.Neurosci. 19 7262 PMID: 10460232

Sakai et al (2010) Augmented PDBu-mediated contaction of bronchial smooth muscle of mice with antigen-induced airway hyperresponsiveness. J.Smooth Muscle Res. 46 259 PMID: 21187674


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1 Citation for Phorbol 12,13-dibutyrate

Citations are publications that use Tocris products. Selected citations for Phorbol 12,13-dibutyrate include:

Nostro et al (2015) Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines. J Cell Biol 4 591 PMID: 25843049


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